Ferdowsi University of Mashhad
A Multi-Faceted Approach for Prediction of Genome Safe Harbor Loci in the Chicken Genome

Nima Dehdilani; Mohsen Fathi Najafi; Hesam Dehghani

Volume 13, Issue 1 , September 2021, Pages 1-9


  To achieve a reliable and persistent expression, the transgene should be precisely integrated into the genome safe harbor (GSH) loci. Little attention has been paid to find the safe harbor loci of the chicken (Gallus gallus domesticus) genome. Identification and characterization of GSH loci that allow ...  Read More

VEGF-C and p53 Gene Expression in the Normal and Neoplastic Mammary Gland of Canines: A Pilot Study

Mohammadreza Nassiri; Azadeh Safarchi; Masoume Vakili-Azghandi; Vinod Gopalan; Mohammad Doosti; Shahrokh Ghovvati; Ahmad Reza Movassaghi

Volume 13, Issue 1 , September 2021, Pages 10-18


  p53 is a tumor suppressor protein that plays an essential role in controlling the cell and vascular endothelial growth factor (VEGF) is one of the most strong and specific angiogenic factors. The main objective of this study was to evaluate the impact of p53 and VEGF-C gene expression in the neoplastic ...  Read More

Molecular Screening of Nitrate Reductase Enzyme in Native Halophilic Bacteria of Iran

Saeedeh Sibevieh; Ensieh Salehghamari; Mohammad Ali Amoozegar; Mohammad Reza Zolfaghari; Mohammad Soleimani; Zohre Nasrollahzadeh; Sara Eftekhari Yazdi

Volume 13, Issue 1 , September 2021, Pages 19-26


  With the increased usage of nitrate fertilizers, the removal of their stable ionic and water-soluble end products is a challenge for human health. Several physicochemical methods have been examined for nitrate removal of water, but biological treatments are mostly preferred due to a higher efficiency ...  Read More

Comparative Analysis of Commercial CCL21 and CCL21/IL1β Recombinant Proteins by in silico Tools

Ahdiyeh Shahtaghi; Ali Alam Shahnabadi; Kamelia Kohannezhad; Neda Amini; Maria Beihaghi

Volume 13, Issue 1 , September 2021, Pages 27-35


  One of the newest diagnostic methods and treatment of cancer is to design new drugs. It is now possible to design a drug with desired properties in theory and evaluate its therapeutic effects through bioinformatics tools. Among the studied drugs, those based on cytokine genes, which increase the body's ...  Read More

Royal Jelly Decreases MMP-9 Expression and Induces Apoptosis in Human Bladder Cancer 5637 Cells

Narges Fazili; Zahra-Soheila Soheili; Saeid Malekzadeh-Shafaroudi; Shahram Samiei; Shamila D.Alipoor; Nasrin Moshtaghi; Abouzar Bagheri

Volume 13, Issue 1 , September 2021, Pages 36-43


  Royal jelly (RJ) from queen honeybee larva as a traditional medicine agent has a variety of pharmacological benefits. In the present study, the effect of Royal jelly was investigated on the urinary bladder cancer cell line (HTB-9 5637). To determine the cell viability in different concentrations ...  Read More

Effect of Glutamine Stability on the Long-term Culture and Line Establishment of Chicken Primordial Germ Cells

Sara Yousefi Taemeh; Jalil Mehrzad; Hesam Dehghani

Volume 13, Issue 1 , September 2021, Pages 44-53


  Primordial germ cells (PGCs) are precursors of mature gametes, which transmit genetic information to the next generation. Due to the importance of PGCs in many fields, including developmental biology, genome editing, transgenesis, and conservation of avian genetic resources, various research aspects ...  Read More

Exploring the Anticancer Efficacy of Mixture of Local Probiotics on MDA-MB-231 and MCF-7 Breast Cell Lines

Mohadese Abdoli; Parisa Fathi Rezaei; Kamran Mansouri

Volume 13, Issue 1 , September 2021, Pages 54-64


  This study aimed to investigate the cytotoxicity of a probiotic mixture on human breast cancer cell lines. To prepare the mixture, local probiotic bacteria were cultured, and the lyophilized supernatant was applied for downstream experiments. The antioxidant activity, total phenol content (TPC), ...  Read More

The Effect of Androgen Deprivation on the Expression of Connexin-43 mRNA in the Heart

Mahnaz Ghowsi; Nazli Khajehnasiri; Sajjad Sisakhtnezhad

Volume 13, Issue 1 , September 2021, Pages 65-71


  Connexin-43 (Cx-43) plays axial roles in the propagation of action potentials and contractile coupling in heart. Down-regulation of Cx-43 in heart is associated with arrhythmia, dilated cardiomyopathy and heart failure. To date, no studies have examined the effects of androgen deprivation therapy (ADT)-induced ...  Read More

Understanding the Effect of Natural Products on Breast Cancer via P53-MDM 2 Signal Pathway

Zahra Ghavidel; Madjid Momeni Moghaddam; Toktam Hajjar; Eisa Kohan-Baghkheirati

Volume 13, Issue 1 , September 2021, Pages 72-80


  The use of medicinal plants in the treatment of diseases has a long history dating back to the presence of humans on earth. Cancer has almost been an incurable disease, and among the various cancers, breast cancer is the most common type of cancer among women and imposes an enormous burden on patients. ...  Read More

Isolation and Characterization of Lytic Bacteriophages Infecting Escherichia coli Antibiotic-Resistant Isolates from Urinary Tract Infections in North-west of Iran

Raheleh Majdani

Volume 13, Issue 1 , September 2021, Pages 81-91


  One of the most prevalent bacterial infections, urinary tract infection (UTI), affects millions of people yearly worldwide. To control the increasing antibiotic-resistant infections, it is essential to introduce alternative approaches such as phage therapy. In this study, isolation, purification, and ...  Read More

Vitamin C Loaded Albumin Nanoparticles Treatment and Its Effect on Collagen I and III and mir-133 Genes Expression

Hamid Reza Shojania; Madjid Momeni Moghaddam; Seyed Ebrahim Hossini

Volume 13, Issue 1 , September 2021, Pages 92-97


  Wound healing is a complex biological process in which many molecules, including microRNA molecules, play an essential role in its regulation. It is well-established that reducing miR-155 expression can accelerate wound healing. This study investigated the effect of using nanoparticles loaded with vitamin ...  Read More