Ferdowsi University of Mashhad

Document Type : Research Articles


1 Biology Department, Faculty of Science, University of Maragheh, Maragheh, Iran

2 Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran


The aim of this study was to investigate the cytotoxicity of probiotic mixture on breast cancer cell lines. A mixture of local probiotic bacteria was cultured and the supernatant was lyophilized for performing additional experiments. The antioxidant activity, total phenol content (TPC) and fatty acid composition of bacterial supernatant (BS) were also measured. The effect of probiotic mixture on cell viability of two breast cancer cell lines and apoptosis induction were investigated using MTT assay and DAPI staining, respectively. The highest antioxidant activity and total phenol content (TPC) of the bacterial supernatant (BS) were detected at 3200 µg/ml. According to the GC–MS analyses, linoleic acid (37.40 %) and oleic acid (26.93 %) were identified as the major fatty acids of the BS. The MTT assay showed that BS has antiproliferative effect on MDA-MB-231 and MCF-7 cells in a time and dose-dependent manner, and also the 3200 μg/ml was determined as IC50. The apoptosis induction was confirmed by morphological characteristics on both cell lines, including nucleus changes, granulation of the nucleus, and the formation of the apoptotic bodies. According to this experiment, cytotoxic activity and apoptosis potential of BS on two breast cancer cell lines including MDA-MB-231 and MCF-7 was shown. BS could be considered as a suitable anticancer agent