Ferdowsi University of Mashhad

Document Type : Research Articles

Authors

Department of Biology, Kavian Institute of Higher Education, Mashhad, Iran

10.22067/jcmr.2021.69999.1005

Abstract

One of the newest diagnostic methods and treatment of cancer is to design new drugs. It is now possible to design a drug with desired properties in theory and evaluate its therapeutic effects through bioinformatics tools. Among the studied drugs; those based on cytokine genes, which increase the body's immunity against cancer, are of great interest. Cytokines are small proteins that play an important role in cell signaling and can affect the function and behavior of surrounding cells. CCL21 chemokine is one of the cytokines that possess antitumor properties has potential on chemoa-ttraction of T lymphocytes and dendritic cells. Interleukin 1 beta (IL1β) is a cytokine involving in different cellular activities such as activation of neutrophils, B-Cells and T-Cells. In the present study, we designed a drug-based cytokine gene to activate T cells and B cells by inserting defined CCL21 epitope and IL1β pepteide sequences into a protein construct. Molecular dynamics simulation was performed in Linux space using Gromex software. Results of RMSD, RMSF and the radius of gyration obtained from the simulation showed the stability of both proteins which indicated that there are no significant conformational differences between the commercial CCL21 and recombinant form. The interaction of synthetic construct and human CCL21 with CCR7 receptor was also investigated by HADDOCK software. Obtained results showed that there are no differences between these proteins and recombinant protein has the same structural and conformational characteristics as human commercial CCL21.

Keywords