Hanieh Jafary; Maryam Ghobeh; Maryam Nouri
Abstract
AbstractIn recent years, various efforts have been made to improve the functional potency of cancer drugs. Due to the fact that Micro RNA in the cell can act as a tumor inhibitor, it can be used as a suitable treatment in most cancers. In this study, chitosan coating is considered as a factor that enhances ...
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AbstractIn recent years, various efforts have been made to improve the functional potency of cancer drugs. Due to the fact that Micro RNA in the cell can act as a tumor inhibitor, it can be used as a suitable treatment in most cancers. In this study, chitosan coating is considered as a factor that enhances the effect of Micro RNA function and effectiveness. MCF-7 cell line was divided into 4 experimental groups, including untreated MCF-7 cell group, MCF-7 cell group with miR encapsulated with chitosan, MCF-7 cell group with chitosan, and MCF-7 cell group with doxorubicin drug as positive control group. The effect of different concentrations of miR-372 was first evaluated, and the optimal dose was selected to evaluate the following parameters: the induction of cell death by applying flow cytometry, the cell survival by MTT, and the level of P53 protein by Immunocytochemistry. The results showed that the dose of 1500 ng / μl of miR-372 coated with chitosan could induce cell death up to 50% in 24 and 72 hours of treatment. In addition, the rate of induction of cell death in the group treated with miR-372 coated with chitosan for 72 hours was statistically significant compared with the control group. In addition, the expression level of P53 protein in the same group was statistically significant compared with the control group. According to the results, the use of cell proliferation cycle regulators such as miR-372 can control the process of proliferation and thus improve the treatment of cancer.
maryam varasteh-kojourian; Ali Ganjeali; Javad asili; Saeid Malekzadeh-Shafaroudi; Akram Taleghani
Abstract
Endophytic fungi are often producing host plant secondary metabolites. Tanshinones are secondary metabolites of the Salvia genus which also produced by some endophytic fungi. Efficient secondary metabolite production in endophytic fungi drops significantly after sequential subcultures. 5-azacytidine ...
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Endophytic fungi are often producing host plant secondary metabolites. Tanshinones are secondary metabolites of the Salvia genus which also produced by some endophytic fungi. Efficient secondary metabolite production in endophytic fungi drops significantly after sequential subcultures. 5-azacytidine (5-AC) is an analog of the naturally occurring pyrimidine nucleoside cytidine and a DNA methyltransferase inhibitor. In this relation, 5-AC is an effective tool to induce expression of silenced secondary metabolite genes in endophytic fungi. In this experiment we isolated 4 endophytic fungi from the roots of Salvia abrotanoides which produced tanshinone. Cryptotanshinone and tanshinon IIA were produced by Penicillium canescens, Penicillium nodositatum, and Penicillium pinophilum, while Paraphoma radicina only produced tanshinon IIA. The maximum amount of tanshinones was extracted from P. pinophilum culture with 130.826 mg cryptotanshinone /g of dry weight and also 50.155 mg Tanshinone IIA/g of dry weight. These amounts were significantly more than the tanshinones produced in plant roots (0.55 mg cryptotanshinone/g of dry weight, 1.3 mg Tanshinone IIA/g of dry weight). In third subculture, tanshinone production decreased significantly. 5-azacytidine as an epigenetic modifier retrieved tanshinone production in 3d subculture of P. pinophilum. Also, 5- azacytidine treatment made a big jump in Tanshinone IIA production in P. radicina (63.176 mg TIIA/g of dry weight) besides increasing Tanshinone IIA production in P. nodositatum cultures. This is the first report using 5- azacytidine to improve tanshinone production in endophytic fungi. Our results confirm that 5- azacytidine is an efficient, easy, and quick chemical to elicitate secondary metabolite production in endophytic fungi.
Roya Dehquan Dehnavi; Seyed Abdolhamid Angaji; Behnaz Beikzadeh; Hengameh Alibeik; Raheleh Roudi; Behzad Narouie
Abstract
Prostate cancer is the second most common malignancy worldwide and is the fifth leading cause of death in men. It is the second most common cancer among urinary tract cancers in Iranian men. The purpose of this study was to evaluate the association of rs10090154 which is located on 8q24 locus and rs1691053 ...
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Prostate cancer is the second most common malignancy worldwide and is the fifth leading cause of death in men. It is the second most common cancer among urinary tract cancers in Iranian men. The purpose of this study was to evaluate the association of rs10090154 which is located on 8q24 locus and rs1691053 on chromosome 5p15.31 with prostate adenocarcinoma, PSA and use them as screening factors if it is associated with prostate cancer.
In this case-control study, 79 patients with prostate adenocarcinoma with an age range of 48 to 86 years and 98 BPH patients with an age range of 47 to 81 years participated. Also, Tetra-primer ARMS-PCR (Tetra-Primer Amplification Refractory Mutation System-Polymerase Chain Reaction) method was used to determine the genotype of each participant.
In this study, no significant difference in genotypic distribution between prostate adenocarcinoma and control groups was observed in rs10090154 (P-value= 0.608) and rs1691053 (P-value = 0.102) polymorphisms. Also, in the study of additive genetics model of rs10090154 polymorphism based on placing TT genotype as reference genotype CC genotype with P-value = 1 and OR{95%CI}= 0.750,{0 .039- 14.576} and CT genotype with P-value= 0.324 and OR{95%CI}= 0.577,{0 .191- 1.739} are not associated with additive genetics model. As well, in rs1691053 based on CC as a reference CT genotype with P-value =0.176 and OR{95%CI}= 0.196,{0.022-1.793} and TT genotype with P-value =0.464, OR{95%CI}= 0.125, {0 .005- 3.225} are not associated with additive genetics model.
the results of this study indicate that rs10090154 and rs1691053 are not associated with prostate cancer in Iranian population.
