Nasrin moshtaghi; Robab Ghahremanzadeh; Seyyed Hasan Marashi
Abstract
Saffron (Crocus sativus L.) is an indigenous and the most valuable and marginal plant in Iran. However,
limited knowledge exists on its molecular biology. The importance of this plant is due to the color, flavor and
medicinal properties of its red stigmas. Saffron stigmas contain a high amount of carotenoids ...
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Saffron (Crocus sativus L.) is an indigenous and the most valuable and marginal plant in Iran. However,
limited knowledge exists on its molecular biology. The importance of this plant is due to the color, flavor and
medicinal properties of its red stigmas. Saffron stigmas contain a high amount of carotenoids such as crocetin
and crocin. Two genes, bch and pds, have essential role in carotenoid production. In this study, the effect of
four different irrigation regimes was evaluated on the expression of bch and pds genes. Semi-quantitative RTPCR
showed no significant difference in the expression levels of genes of interest related to the internal
standard (18S rRNA). Results of Real-Time PCR assays showed that the expression of bch and pds genes were
affected by irrigation treatments as their expression decreased in irrigated plants in comparison to non irrigated
ones, exept for one irrigation treatment (one irrigation in September) where the pds gene expression showed
higher level. However, the expression profile of the genes was almost the same in all treatments. The
comparison between results of two techniques indicated that the Real-Time PCR is more accurate for
determination of the level of transcript in the Iranian saffron. It was interesting that by decreasing of irrigation,
the expression level of these two genes increased indicating that abiotic stress and drought can affect on the
gene expression relating to the saffron color.
Moslem Shojaei; Mohammadreza Mohammad Abadi; Masood Asadi Fozi; Omid Dayani; Amin Khezri; Masoumeh Akhondi
Abstract
Identification of genes affecting energy balance, milk yield and feed intake is an interesting area of
researches in animal breeding. Leptin gene polymorphism is associated with key economic affair. Considering
rich resources for animals, in our country, accomplishing a few assays to identify a gene ...
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Identification of genes affecting energy balance, milk yield and feed intake is an interesting area of
researches in animal breeding. Leptin gene polymorphism is associated with key economic affair. Considering
rich resources for animals, in our country, accomplishing a few assays to identify a gene that controls her traits
with molecular genetics, and identifying the candidate genes in sheep breeds using DNA test can greatly help
to her breeding progress. For analyzing Leptin gene polymorphism and its association with growth traits in
Kermani sheep, blood samples of 120 sheep of both genes rearing at breeding centre of Shahre Babak were
taken. In addition growth traits were measured. PCR was performed to amplify 275 bp fragments of exon 3
from Leptin gene. Then Single Strand Conformation Polymorphism (SSCP) of PCR product was performed
and Leptin band patterns (genotypes) were obtained using acrylamid gel and silver staining. For Leptin gene 10
genotypes including A/A, C/C, A/B, A/C, A/B/C, A/B/E, A/B/F, A/C/F, A/B/D/E and A/B/C/F were obtained.
The results of this study showed that the growth traits are significantly affected by the genotypes. Accordingly,
A/B/E, A/C, A/B/C/F and A/B/C/F genotypes had higher body weight at 3, 6, 9 and 12 months of ages
respectively. The animals with A/B, A/B, A/B/F and A/B/D/E genotypes had the smallest body weight at 3, 6,
9 and 12 months of ages respectively. It is suggested that polymorphism in Leptin gene loci can be used as a
selective criterion to improve growth traits in Kermani sheep.
aliakbar ehsanpour; Behrokh Shojaie; Mohammad Reza Abdi
Abstract
In this study, the effects of gamma rays on changes of proline, sodium and potassium in potato calli
(Solanum tuberosoum L.) cultivar "White Desiree" were investigated. After irradiation of calli by 60 and 80 Gy
of gamma radiation, irradiated and non irradiated calli were transferred to a callus production ...
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In this study, the effects of gamma rays on changes of proline, sodium and potassium in potato calli
(Solanum tuberosoum L.) cultivar "White Desiree" were investigated. After irradiation of calli by 60 and 80 Gy
of gamma radiation, irradiated and non irradiated calli were transferred to a callus production medium
containing 0, 30, 60, 90, 120 and 150 mM NaCl. After 26 days post treatment amounts of proline, Na+ and K+
were measured. The results showed that generally in each of gamma ray treatment, amounts of proline at 120
and 150 mM NaCl were significantly more than 0, 30, 60 and 90 mM salt. A significant difference observed
only at 150 mM NaCl between irradiated and non irradiated calli. This result could be related to the proline
role in osmotic adjustment at high concentration of salt. The amounts of sodium increased by increasing salt
the concentration but the amount of potassium decreased. These results showed that the sodium influx
inhibited the potassium uptake, and may be because of strong inhibitory effect of sodium on the potassium
uptake system or efflux potassium from the cells. The Na+/K+ ratio decreased at 150 mM by 60 and 80 Gy of
gamma radiation.
Mahnaz Kiani; Homa Zarghami; Ali Tehranifar; Farshid Memariani
Abstract
In vitro methods provide a variety of tools to supplement traditional methods for collection, propagation and
preservation of endangered plant species. In this study, an efficient protocol was developed for in vitro
propagation of Colutea gifana, a rare and endangered plant species with limited reproductive ...
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In vitro methods provide a variety of tools to supplement traditional methods for collection, propagation and
preservation of endangered plant species. In this study, an efficient protocol was developed for in vitro
propagation of Colutea gifana, a rare and endangered plant species with limited reproductive capacity that
grows in a narrow area of Iran. Single node explants were used for a series of experiments to select the
appropriate disinfection method and growth regulators for establishment, proliferation and rooting stages.
Explants showed the highest establishment percent after treatment with 2% Sodium hypochlorite (NaOCl) for
15 min, cultured in MS medium containing 2.2 μM 6-benzylaminopurine (BA) and 1 μM indole-3-butyric acid
(IBA). In proliferation stage, 8.8 μM of BA was more effective cytokinin than Kinetin (Kin) and Thidiazuron
(TDZ) for growth induction of axillary shoots. In vitro rooting of proliferated shoots was induced in halfstrength
MS medium in all concentrations of both tested auxins i.e. IBA and α-naphthalene acetic acid (NAA).
Eighty percent of the plantlets were successfully acclimatized to ex vitro conditions, showed normal
development. These plants were used to replenish declining populations in the collection sites and conserve C.
gifana from extinction.
Akram Zia; Farkhonde Rezanejad; Abbas Safarnejad
Abstract
Thyme (Thymus vulgaris L.) is an aromatic and medicinal plant which is very important for the herbal
industry. In this research, in vitro selection for NaCl tolerance was investigated in this plant. Hypocotyl and
shoot explants (apical meristem and cotyledonary leaves) of sterilized seedlings were ...
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Thyme (Thymus vulgaris L.) is an aromatic and medicinal plant which is very important for the herbal
industry. In this research, in vitro selection for NaCl tolerance was investigated in this plant. Hypocotyl and
shoot explants (apical meristem and cotyledonary leaves) of sterilized seedlings were cultured in MS medium
supplemented with different combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D), napthaleneacetic acid
(NAA), kinetin (KIN) and N6-benzyladenine (BA) for callus induction. Results showed that the maximum
frequency of callus induction was obtained on MS medium containing 1:0.5 ratio of 2, 4-D:Kin (mgl-1), while
optimal callus induction with the best quality and regeneration potential was achieved in 1:1 ratio of NAA:BA
(mgl-1). The calli grown in 1:1 ratio of NAA:BA (mgl-1) were transferred to NaCl supplemented medium at 50,
75 and 100 mM concentrations. Fresh and dry weights, percentage of necrosis and regeneration of calli were
determined after 4 weeks. There were significant differences between fresh and dry weights of calli in different
concentrations of NaCl. The highest and the lowest of fresh and dry weights of calli from hypocotyl explants
were observed in 0 (control) and 100 mM NaCl concentrations, respectively. But fresh weight of shoot-derived
calli induced from shoots reduced only in 100 mM NaCl and the dry weight of them was not significantly
different. The necrosis percentage increased by increasing the salt concentration. Callus regeneration just
occurred at the concentration of 50 mM of NaCl and all regenerated shoots well rooted on half strength MS
medium, with 0.8% (w/v) sucrose and without growth regulators.
Reihaneh Sabbaghzadeh; Majid Monajjemi; Fatemeh Mollaamin; Shahrbano Oryan
Abstract
Insulin-like growth factor (IGF-1) is an anti-apoptosis factor in multiple cell types associated with various
cancers. Computational methods allow investigating the systems between 50–100 atoms in the frame of
quantum mechanics and up to 50,000 atoms with molecular dynamics. Since there are specific ...
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Insulin-like growth factor (IGF-1) is an anti-apoptosis factor in multiple cell types associated with various
cancers. Computational methods allow investigating the systems between 50–100 atoms in the frame of
quantum mechanics and up to 50,000 atoms with molecular dynamics. Since there are specific interactions
between the residues, the solvent could play an important role in the stability of the native structure. Therefore
it is useful to carry out such simulations at atomistic detail. MC, MD and LD simulations of the IGF-1 were
performed with the HyperChem7.0 program. The geometries, and the interaction energies, bonds, angles,
stretch-bends, electrostatic and the VDW Interactions were carried out in solution and gas phase. We have
computed the transition temperature for the IGF-1 molecule. Studying the changes occurred in the potential
energy of the three force fields showed that Amber force field is better than MM+ and OPLS force field and
also MD simulation, at least in this model, is more effective than MC and LD methods. After equilibration, the
MD simulation was very stable, and the difference between the relation coefficients R2=0.8173 in gas and
R2=0.7558 in water was compared. The Pearson correlation suggests that there is an inverse relationship (R=-
0.25) between in vitro temperature and stability of the structure.
