Document Type : Research Articles
1 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad
2 Industrial Biotechnology Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
Today, ostrich breeding has been widely developed in Iran and other countries due to the ability of this animal to produce quality meat, leather, and oil. However, one of the main problems in breeding them is sex determination using aggressive techniques with low accuracy. This study aimed to determine the sex of immature ostriches using specific primers in a multiplex PCR reaction. This study considered 20 specimens of unspecified immature and six specimens (three adult males and females) of known-sex African ostriches as controls. SS and OSFES primers were used to amplify part of the female-specific sequence and 18S primer was used as a control in a PCR reaction. The presence of SS and OSFES bands in gel electrophoresis indicated the amplification of the desired parts related to the female sex and the absence of these bands indicates the male sex of the species. In total, out of 20 African ostriches studied, 50% of them belonged to females and 50% of them belonged to males. Later, with the growth of immature individuals, the results of this experiment were confirmed. In this study, it was found that the use of feather samples for DNA extraction and multiplex PCR is a suitable, accurate, and cost-effective method in identifying and determining the sex of young ostrich and leads to more real and reliable results, avoiding stress in birds.
Ellergren, H. (1996) First gene on the avian W chromosome (CHD) provides a tag for universal sexing of non-ratite birds. Proceedings of the Royal Society of London. Series B: Biological Sciences 263(1377):1635-1641. Griffiths, R., Daan, S. and Dijkstra, C. (1996) Sex identification in birds using two CHD genes. Proceedings of the Royal Society of London. Series B: Biological Sciences 263(1374):1251-1256.
Gruszczynska, J., Alama, A., Miasko, M., Florczuk-Kolomyja, P. and Grzegrzólka, B. (2019) Molecular identification of sex in the monomorphic breed of pigeons. Indian Journal of Animal Research, 53:1577-1582. Hallam, M. G. (1992) The Topaz introduction to practical ostrich farming. Hallam, Harare publication.
Javanmard, A. and Javadmanesh, A. (2005) Detection of butchers fraud in meat markets with bovine carcass sex determination by means of PCR. The Proceedings of the 4th National Biotechnology Congress, August 2005, Kerman, Iran.
Jia, X. X., Lu, J. X., Tang, X. J., Fan, Y. F. & Gao, Y. S. (2023) A new method for molecular sex identification in the emu (Dromaius novaehollandiae), British Poultry Science, DOI: 10.1080/00071668.2022.2163876.
Kreibich, A. and Sommer, M. (1995) Ostrich farm management. Landwirtschaftsverlag GmbH.
Markoulatos, P., Siafakas, N. and Moncany, M. (2002) Multiplex polymerase chain reaction: a practical approach. Journal of clinical laboratory analysis, 16(1):47-51. Medaglia, A. and Henrique-Silva, F. (2005) New PCR multiplexes for sex typing of ostriches. Brazilian Journal of Biology 65(4):743-745.
Mine, O. M., Mochakana, M. E., Mpapho, T., Motlhanka, D. T. M. and Kgwatalala, P. (2002).Application of a sex identification technique in juvenile ostriches and its potential application in Botswana. South African Journal of Animal Science 32(3): 160-163. Poławska, E., Marchewka, J., Cooper, R. G., Sartowska, K., Pomianowski, J., Jóźwik, A., et al. (2011) The ostrich meat–an updated review. Animal Science Papers and Reports 29(2):89-97. Shanawany, M. M. and Dingle, J. (1999) Ostrich production systems. Food & Agriculture Org.
Tsuda Y, Nishida-Umehara C, Ishijima J, Yamada K, Matsuda Y. (2007) Comparison of the Z and W sex chromosomal architectures in elegant crested tinamou (Eudromia elegans) and ostrich (Struthio camelus) and the process of sex chromosome differentiation in palaeognathous birds. Chromosoma 116(2):159–173
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