Ferdowsi University of Mashhad

Document Type : Research Articles


1 Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran


Immediate intervention with minimal side effects is the most significant factor for the enhancement of wound healing. However, a majority of drugs used for this purpose are chemical-based containing various compounds, such as sulfite, which sometimes cause allergic reactions in a number of patients, or anti-inflammatory agents that cause elevated blood sugar and weight gain. Hence, many researchers look for natural compounds, such as glycoproteins, not only to reduce the side effects but also to improve the speed of healing.
In this study, we have created a natural biological dressing using the combination of extracellular matrix (ECM) derived from articular cartilage and DH5α bacterial ghost (BG). Both articular cartilage and BG contain high amounts of collagen and glycoproteins, and proteoglycans, respectively. The experimental wound on the rabbit pinna treated by the biological dressing. Then microbial, scanning electron microscopy and microscopic analyses measured the wound healing parameters, including the number of fibroblast cells, the collagen contents, percentage of wound closure, and the number of colonies.
The results confirmed ECM (OC), BG (OG) and their mixture (OGC) groups has better effects than control groups. Histological parameters, such as numbers of fibroblast cells and amount of collagen fibers, represented greater degree of wound healing in OGC group compared with OC, OG, and control groups. Our findings proved that ECM and bacterial ghost effectively increased the rate of wound healing. The mixture of ECM and BG provides a biological dressing that could be used in wound repair in the future.