Ferdowsi University of Mashhad

Document Type : Research Articles


1 1) Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran, 2) Department of Marine Sciences, Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran

2 Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran

3 Department of Marine Sciences, Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran


     The Caspian Sea is the largest inland body of water in the world and so has both common characteristics of seas and lakes with over 153 fish species which inhabit the sea and its basin. However, little is known about the trace element (TE) contaminations (TECs) in its tissues. In the present study, 122 specimens of three fish species including Rutilus caspius (Roach, n=71), Leuciscus aspius (Asp, n=20), and Tinca tinca (Tench, n=31) were collected from three different fisheries regions (i.e. Astara, Anzali and Kiashahr) of the southern part of the Caspian Sea from September 2017 to June 2018. Inductively coupled plasma optical emission spectrometry (ICP-OES) was employed to measure TE levels in different fish tissues. An attempt was made to assess possible influences of habitat on element accumulation in the liver and kidney of three fish species in the southwest of the Caspian Sea basin. Some elements including Ca, K, Mg, P, S, Sc, and Sr showed different concentrations in the liver and kidney. Also their levels were significantly different between freshwater resident (Tench) and marine (Roach) species (p < 0.05). The differences among TECs in the liver and kidney of Roach, Asp and Tench were reduced to three components using principal component analysis (PCA). Results indicated that 83.60% of the total variability is related to TEs such as Cu, Fe, Sr, Ca, S, Na, Mg, K, and Al. The impact of habitat variability on the element accumulation was confirmed through linear chart obtained for liver and kidney (as body filtering organs) of Roach and Asp as marine residents as well as Tench as a freshwater resident. This could illustrate the borderline created by these habitats.


Abtahi B. and Sabbagh kashani A. (2005) Assessing Pb, Ni and Zn accumulation in the tissues of Liza aurata in the south Caspian Sea. Iranian Scientific Fisheries Journal 14: 65-78.

Afonso A., Gutierrez A. J., Lozano G., Gonzalez-Weller D., Rubio C., Caballero J. M., Hardisson A. and Revert C. (2017) Determination of toxic metals, trace and essentials, and macronutrients in Sarpa salpa and Chelon labrosus: risk assessment for the consumers. Environmental Science and Pollution Research 24(11): 10557-10569.

Alamdar A., Eqani S. A. M. A. S., Hanif N. Ali S. M., Fasola M., Bokhari H., Katsoyiannis I. A. and Shen H. (2017) Human exposure to trace metals and arsenic via consumption of fish from river Chenab, Pakistan and associated health risks. Chemosphere 168: 1004-1012.

Aliko V., Qirjo M. and Sula E. (2018) Antioxidant defense system, immune response and erythron profile modulation in Gold fish, Carassius auratus, after acute manganese treatment” Fish Shellfish Immunology 76:101–109.

Alipour H. and Banagar G. h. R. (2018) Health risk assessment of selected heavy metals in some edible fishes from Gorgan Bay, Iran. Iranian Journal of Fisheries Sciences 17(1): 21-34.

Amini Ranjbar G. h. and Sotudehnia F. (2005) Investigation of heavy metals accumulation in muscle tissue of Mugil auratus in relation to standard length, weight, age and sex. Iranian Scientific Fisheries Journal 14: 1-18. (In Persian).

Askary Sary A. and Beheshti M. (2012) Cadmium, iron, lead and mercury bioaccumulation in Abu Mullet, Liza abu, different tissues from Karun and Karkheh Rivers, Khozestan, Iran. Bulletin of Environmental Contamination and Toxicology 88: 158-161.

Azevedo J., Fernandez W., Farias L., Favaro D. and Braga E. (2009) Use of Cathorops spixii as bioindicator of pollution of trace metals in the Santos Bay, Brazil. Ecotoxicology 18(5): 577-586.

Beheshti M. (2011) Comparative study of concentration of heavy metals (Cu, Fe, Zn, Mn) in muscle, liver and gill organ of fish (Liza abu) in the Karoon and Karkheh rivers in Khuzestan province.” M.Sc. Thesis. Islamic Azad University, Science and Research, Ahwaz. (In Persian).

Bibak M., Sattari M., Agharokh A., Tahmasebi S. and Imanpour Namin J. (2018) Assessing some heavy metals pollutions in sediments of the northern Persian Gulf (Bushehr province). Environmental Health Engineering and Management Journal 2018: 5(3): 175–179.

Boran M., Karacam H., Celikkale M. S. and Kose S. (2000) Levels of heavy metals in blue whiting caught from the eastern Black Sea area of Turkey. Toxicological and Environmental Chemistry 2000: 75, 67-73.

Burgos-Aceves M. A., Cohen A., and Smith Y. (2018) MicroRNAs and their role on fish oxidative stress during xenobiotic environmental exposures. Ecotoxicology and Environmental Safety. 148: 995-1000.

Capillo G., Silvestro S. and Sanfilippo M. (2018) Assessment of electrolytes and metals profile of the Faro Lake (Capo Peloro Lagoon, Sicily, Italy) and its impact on Mytilus galloprovincialis. Chemestry & Biodiversity.

Chorehi M. M., Ghaffari H., Hossaini S. A., Niazie E. H. N., Vajargah M. F. and Hedayati A. (2013) Acute toxicity of Diazinon to the Caspian vimba, Vimba vimba persa (Cypriniformes: Cyprinidae). International Journal of Aquatic Biology 1(6): 254-257.

De Mora S., Sheikholeslami M. R., Wyse E. and Azemard S. (2004) An assessment of metal contamination in coastal sediments of the Caspian Sea. Marine Pollution Bulletin 48: 61-77.

Ebrahimzadeh M. A., Eslami S. and Nabavi S. F. (2011) Determination of trace element level in different tissues of the leaping mullet (Lizasaliens, Mugilidae) collected from Caspian Sea. Biological Trace Element Research 144: 804-811.

Eslami S., Moghaddam A. H. and Jafari N. (2011) Trace element level in different tissues of Rutilus frisii kutum collected from Tajan River, Iran. Biological Trace Element Research 143: 965-973.

Eslami V., Sattari M. and Namin J.I. (2014) Concentration of heavy metals (Pb, Cd) in muscle and liver of Perca fluviatilis and Tinca tinca in Anzali Wetland, southwest the Caspian Sea. International Journal of Aquatic Biology 2(6): 319-324.

Fazio F., Piccione G. and Tribulato K. (2014) Bioaccumulation of heavy metals in blood and tissue of striped mullet in two Italian lakes. Journal of Aquatic Animal Health 26: 278–284.

Khanipour A. A., Ahmadi M. and Seifzadeh M. (2018) Study on bioaccumulation of heavy metals (cadmium, nickel, zinc and lead) in the muscle of wels catfish (Silurus glanis) in the Anzali Wetland. Iranian Journal of Fisheries Sciences 17(1): 244-250.

Marijić V. F. and Raspor B. (2007) Metallothionein in intestine of red mullet, Mullus barbatus as a biomarker of copper exposure in the coastal marine areas. Marine pollution bulletin 54(7): 935-940.

Munoz-Olivas R. and Camara C. (2001) Speciation related to human health, In: L, Ebdon, L, Pitts, R, Cornelis, H, Crews, OFX, Donad, P, Quevauviller, (Eds.), Trace Element Speciation for Environment, Food and Health. The Royal Society of Chemistry 331-353.

Ohji M., Arai T. and Miyazaki N. (2007) Comparison of organotin accumulation in the masu salmon Oncorhynchus masou accompanying migratory histories. Estuarine, Coastal and Shelf Science 72(4): 721-731.

Ohji M., Harino H. and Arai T. (2006) Differences in organotin accumulation among ecological migratory types of the Japanese eel Anguilla japonica. Estuarine, Coastal and Shelf Science 69(1-2): 270-290.

Pagano M., Porcino C. and Briglia M. (2017) The influence of exposure of cadmium chloride and zinc chloride on haemolymph and digestive gland cells from Mytilus galloprovincialis. International Journal of Environmental Research 11(2): 207-216.

Pourang N. and Dennis J. H. (2005) Distribution of trace elements in tissues of two shrimp species from the PersianGulf and roles of metallothionein in their redistribution. Environment International 31(3):325–341.

Sadeghirad M. (2007) Accumulation of trace elements in Acipenser persicus tissues in relation to feeding habits and mode of absorption of these pollutants. Iranian fisheries research organization. Internatinoal sturgeon research institut. 42P.

Salgado-Ramirez C. A., Mansilla-Rivera I. and Rodriguez-Sierra C. J. (2017) Comparison of trace metals in different fish tissues of Scomberomorus spp.(“sierra”) and Lutjanus synagris (“arrayado”) from Jobos Bay and La Parguera coastal areas in southern Puerto Rico. Regional studies in marine science 13: 1-11.

Sattari M., Imanpour J., Bibak M., Forouhar Vajargah M. and Khosravi A. (2019) Investigation of metal element concentrations in tissue of Rutilus frisii in the Southwest Caspian Sea. Iranian Scientific Fisheries Journal 28(3): pp.149-161.

Sattari M., Namin J. I., Bibak M., Vajargah M.F., Bakhshalizadeh S. and Faggio, C. (2019) Determination of Trace Element Accumulation in Gonads of Rutilus kutum (Kamensky, 1901) from the South Caspian Sea Trace Element Contaminations in Gonads. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences

Sattari M., Namin J. I., Bibak M., Vajargah M. F., Faggio C. and Haddad M. S. (2019) Trace and macro elements bioaccumulation in the muscle and liver tissues of alburnus chalcoides from the south Caspian Sea and potential human health risk assessment. Journal of Energy, Environmental & Chemical Engineering 4(1):13.

Sattari M., Namin J. I., Bibak M., Vajargah M.F., Hedayati A., Khosravi A. and Mazareiy M.H. (2019) Morphological comparison of western and eastern populations of Caspian kutum, Rutilus kutum (Kamensky, 1901)(Cyprinidae) in the southern Caspian Sea. International Journal of Aquatic Biology 6(4): 242-247.

Seco-Gesto E. M., Moreda-Piñeiro A. and BermejoBarrera A. (2007) Multi-element determination in raft mussels by fast microwave assisted acid leaching and inductively coupled plasma-optical emission spectrometry, Talanta. 72: 1178.

Shahryari A., Golfirozy K., Noshin S. (2010) Muscular concentration of cadmium and lead in carp, mullet and kutum of the Gorgan Bay, Caspian Sea. Iranian Journal of Fisheries Sciences 19(2): 95-100.

Tiphaine M., Pierre C., Tiphaine C., Paco B., Christophe B.P., Sandrine B., Emmanuelle R. and Marc B. (2018) Trace metal concentrations in the muscle of seven marine species: Comparison between the Gulf of Lions (North-West Mediterranean Sea) and the Bay of Biscay (North-East Atlantic Ocean). Marine pollution bulletin 135: 9-16.

Trevizani T. H., Domit C., Vedolin M. C., Angeli J. L. F. and Figueira R. C. L. (2019) Assessment of metal contamination in fish from estuaries of southern and southeastern Brazil. Environmental monitoring and assessment 191(5): 308.

Türkmen M., Türkmen A. and Tepe Y. (2007) Metal contaminations in five fish species from Black, Marmara, Aegean and Mediterranean seas, Turkey. Journal of the Chilean Chemical Society 52: 4, 1314-1318.

Tzeng W., Shiao J., and Iizuka Y. (2002) Use of otolith Sr: Ca ratios to study the riverine migratory behaviors of Japanese eel Anguilla japonica. Marine Ecology Progress Series 245: 213-221.

Vajargah M. F., Hedayati A., Yalsuyi A. M., Abarghoei S., Gerami M. H. and Farsani, H. G. (2014) Acute toxicity of butachlor to caspian kutum (Rutilus frisii Kutum Kamensky, 1991). Journal of Environmental Treatment Techniques 2(4): 155-157.

Walther B. D. and Thorrold S. R. (2006) Water, not food, contributes the majority of strontium and barium deposited in the otoliths of a marine fish. Marine Ecology Progress Series 311: 125-130.

Zhou J. L., Salavador S. M. and Liu Y. P. (2001) Heavy metals in the tissues of common dolphins (Delphinus delphis) stranded on the Portuguese coast. Science of the Total Environment 273: 61.