Maryam Nouri; Hanieh Jafary; Maryam Ghobeh
Abstract
In recent years, various efforts have been made to improve the functional potency of cancer drugs. Due to the fact that microRNA in the cell can act as a tumor inhibitor, it can be used as a suitable treatment for most cancers. In this study, chitosan coating is considered a factor that enhances function ...
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In recent years, various efforts have been made to improve the functional potency of cancer drugs. Due to the fact that microRNA in the cell can act as a tumor inhibitor, it can be used as a suitable treatment for most cancers. In this study, chitosan coating is considered a factor that enhances function and effectiveness of microRNAs. MCF-7 cell line was divided into four experimental groups, including an untreated MCF-7 cell group, MCF-7 cell group with miR encapsulated with chitosan, MCF-7 cell group with chitosan, and MCF-7 cell group with doxorubicin as a positive control group. The effect of different concentrations of miR-372 was first evaluated, and the optimal dose was selected to evaluate the following parameters: the induction of cell death by applying flow cytometry, the cell survival by MTT, and the level of P53 protein by immunocytochemistry. The results showed that the dose of 1500 ng/μl of miR-372 coated with chitosan could induce cell death up to 50% in 24 and 72 hours of treatment. In addition, the rate of induction of cell death in the group treated with miR-372 coated with chitosan for 72 hours was statistically significant compared with the control group. In addition, the expression level of P53 protein in the same group was statistically significant compared with the control group. According to the results, the use of cell proliferation cycle regulators such as miR-372 can control the process of proliferation and thus improve the treatment of cancer.
Mohadese Abdoli; Parisa Fathi Rezaei; Kamran Mansouri
Abstract
This study aimed to investigate the cytotoxicity of a probiotic mixture on human breast cancer cell lines. To prepare the mixture, local probiotic bacteria were cultured, and the lyophilized supernatant was applied for downstream experiments. The antioxidant activity, total phenol content (TPC), ...
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This study aimed to investigate the cytotoxicity of a probiotic mixture on human breast cancer cell lines. To prepare the mixture, local probiotic bacteria were cultured, and the lyophilized supernatant was applied for downstream experiments. The antioxidant activity, total phenol content (TPC), and fatty acid composition of the bacterial supernatant (BS) were also measured. The possible cytotoxic/anti-proliferative effect of the probiotic mixture was accessed on both breast cancer cell lines at different concentrations using MTT assay. Furthermore, the apoptosis-inducing effects of the same mixture was studied by DAPI staining. The highest level of antioxidant activity and total phenol content (TPC) were detected for the BS at 3200 µg/ml. According to the GC–MS analysis, linoleic acid (37.40 %) and oleic acid (26.93 %) were identified as the major fatty acids of the BS. The MTT assay showed that the BS has anti-proliferative effects on MDA-MB-231 and MCF-7 cells in a time- and dose-dependent manner (IC50: 3200 μg/ml). The apoptosis-inducing effects of the mixture was confirmed in both cell lines through morphological analyses of the cells’ nucleoli, and the formation of apoptotic bodies. According to these experiments, cytotoxic effects and apoptosis-inducing potential were confirmed for the BS against two human breast cancer cell lines, including MDA-MB-231, and MCF-7. Hence, it could be considered as a suitable anti-cancer agent.
Maryam Rezaeigazik; Mohammad Nabiuni; Hanieh Jalali; Majid Kabuli
Abstract
Morphine as an analgesic drug is used frequently in cancer patients. Contradictory results have been achieved from previous studies related to morphine effects in different concentrations. In current study, we examined the effect of clinical concentrations of morphine on A2780Cp cell line related to ...
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Morphine as an analgesic drug is used frequently in cancer patients. Contradictory results have been achieved from previous studies related to morphine effects in different concentrations. In current study, we examined the effect of clinical concentrations of morphine on A2780Cp cell line related to ovarian cancer. Moreover, its effect on the cytotoxicity of cisplatin was investigated. A2780CP cells were cultured in RPMI1640 medium and treated with clinical doses of morphine alone or in combination with cisplatin. The rate of cell proliferation was measured using MTT assay, morphological changes of nuclei were revealed by 4′,6-diamidino-2-phenylindole (DAPI) staining, and expression of B-cell lymphoma 2 (Bcl-2) was measured using flowcytometry. MTT assay results showed clinical concentration of morphine had no effect on viability of A2780CP cells and toxicity of cisplatin. DAPI staining revealed no chromatin condensation in presence of morphine, and flowcytometry analysis showed that the expression of Bcl-2 in treated cells did not differ from control cells. In accordance with findings in other kinds of cancer, our results demonstrated that morphine did not interact with the function of cispatin in ovarian cancer. This finding can be considered in clinical applications of morphine.
Atieh Teymoori; Mojtaba Teimoori; Madjid Momeni-Moghaddam
Abstract
For 50 years, the term gene is synonymous with regions of the genome gene that coding by mRNAs and translate to protein. nonetheless, Genome wide Recent studies have revealed that regulating gene expression through degradation or translational inhibition of their point mRNAs and thus attend in a wide ...
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For 50 years, the term gene is synonymous with regions of the genome gene that coding by mRNAs and translate to protein. nonetheless, Genome wide Recent studies have revealed that regulating gene expression through degradation or translational inhibition of their point mRNAs and thus attend in a wide variety of physiological and pathological cellular processes including: development, cell proliferation, differentiation, and apoptosis pathways by thousands of regulatory non coding RNA such as lncRNAs and microRNAs. According to a recent survey, it is known this RNAs have vital role in regulation cellular pathways at transcriptional, posttranscriptional and epigenetic levels. These noncoding genes are often aberrantly expressed in a variety of human cancers. However, the biological functions of most ncRNAs remain largely in doubt. In this review, we proved that a remarkable part of the genetic etiology of cancer is imposed by noncoding regulatory sequences. The purpose of this review is aimed to give an outlook of using of noncoding RNA as diagnostic markers and therapeutic targets. These observations emphasized that the recognition of coding genes and Research continued evolution and function of non-coding RNAs for a comprehensive understanding human complex diseases like cancer are essential.
