Monireh Bahrami; Muhammad Irfan-Maqsood
Abstract
LncRNAs (long non-coding RNAs) are responsible to control the degradation process, RNA stability, orchestration, inhibition, transcription and histone modification etc. These RNAs have been termed as the key agents of several mechanisms such as development, organogenesis and regeneration of damaged tissues ...
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LncRNAs (long non-coding RNAs) are responsible to control the degradation process, RNA stability, orchestration, inhibition, transcription and histone modification etc. These RNAs have been termed as the key agents of several mechanisms such as development, organogenesis and regeneration of damaged tissues etc. They interact with a number of partner molecules either protein, RNAs or DNAs and control the cellular behaviour while differentiation or maintaining the stem cell status. This editorial is discussing the significance of lncRNAs as therapeutic target in stem cell therapy field.
Maryam Moghaddam Matin; Morvarid Saeinasab; Saeideh Nakhaei-Rad; Mahdi Mirahmadi; Nasser Mahdavi Shahri; Mahmoud Mahmoudi; Ahmad Reza Bahrami
Abstract
Abstract
Regeneration is a biological phenomenon, which takes place via two main mechanisms: first,
dedifferentiation of mature cells followed by their differentiation into functional new cells and second,
activation of endogenous somatic stem cells for regeneration of damaged or lost tissues. One ...
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Abstract
Regeneration is a biological phenomenon, which takes place via two main mechanisms: first,
dedifferentiation of mature cells followed by their differentiation into functional new cells and second,
activation of endogenous somatic stem cells for regeneration of damaged or lost tissues. One of the best
examples of healing process in mammals is the regeneration of damaged pinna in rabbits by blastema tissue.
The aim of present study was to investigate culture requirements, proliferative properties and expression of
some stemness factors in cells derived from regenerating blastema tissue obtained from rabbit pinna in vitro.
The regenerating tissues were obtained from male New Zealand white rabbits by double punching of the pinna
and cell culture conditions were set to derive and enrich the self renewing cells for further characterisation. The
cells were subjected to survival and growth examinations in vitro, and expression of several stemness factors
was studied in these cells using reverse transcription polymerase chain reaction (RT-PCR). Results revealed
that the derived cells are rather immortal, as they have been growing for more than 120 passages in culture up
until this report. Furthermore, RT-PCR and flow cytometry analyses showed that these cells express a number
of stemness related genes including Oct4 and Sox2. In conclusion, in this study, stem like cells were derived
from blastema tissue of rabbit ears for the first time, showing great self renewing capacity, which provides a
suitable in vitro model for regeneration studies. Moreover, they could be considered as a good source of stem
like cells for future applications.
