Roya Karamian; Fatemeh Ghasemlou
Abstract
Plant regeneration was achieved in Verbascum speciosum Schard. via organogenesis and somatic
embryogenesis by culture of mature embryo explants. Two types of calli, embryogenic and non-embryogenic,
were induced from mature embryo explants on Murashige and Skoog (MS) medium supplemented with
different ...
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Plant regeneration was achieved in Verbascum speciosum Schard. via organogenesis and somatic
embryogenesis by culture of mature embryo explants. Two types of calli, embryogenic and non-embryogenic,
were induced from mature embryo explants on Murashige and Skoog (MS) medium supplemented with
different concentrations of benzyl adenine (BA) and α-naphthalene acetic acid (NAA). In order to further
proliferate the somatic embryoids, the yellow and friable embryogenic calli were transferred on MS medium
containing 0.5 mg-1 charchol and 0.1 or 1 mg-1 2,4-dichlorophenoxy acetic acid (2,4-D) or into MS medium
containing 60 g-1 sucrose, 50 mgl-1 casein hydrolysate (CH), 0.5 mg-1 kinetin (Kin), 5 mg-1 2,4-D and 0.5 mg-1
charchol. Among the 3 tested media, MS medium containing 0.1 mg-1 2,4-D and 0.5 mg-1 charchol was more
effective for proliferation of embryonic calli. Somatic embryos were transferred to hormone free MS medium
for maturation and shoot regeneration. In addition, shoots and roots regenerated from non-embryogenic calli in
hormone free MS medium or containing NAA and BA. Shoot buds were obtained from non-embryogenic calli
and they were transferred to MS medium supplemented with 1 mg-1 BA or Kin for further growth and
multiplication. Regenerated plants then were potted and maintained in the greenhouse.
Masood Ranjbar; Zahra Hajmoradi; Roya Karamian
Abstract
In the present paper, the cytogenetic study including meiotic chromosome number and behavior along with
pollen viability were performed in 4 populations of Trigonella spruneriana Boiss. This is the first cytogenetic
report of the taxon. All populations are diploid and possess 2n = 2x = 16 chromosome ...
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In the present paper, the cytogenetic study including meiotic chromosome number and behavior along with
pollen viability were performed in 4 populations of Trigonella spruneriana Boiss. This is the first cytogenetic
report of the taxon. All populations are diploid and possess 2n = 2x = 16 chromosome number, which is
consistent with the proposed basic number of x = 8. In addition, some meiotic irregularities observed in
different populations included chromosomes stickiness, B-chromosomes, chromosome bridges resulting from
stickiness, the occurrence of laggard chromosomes, formation of micronuclei in tetrad cells and cytomixis. The
highest and the lowest percentages of pollen viability were observed in populations SPR 658 and SPR 566,
respectively.
Masoud Ranjbar; Roya Karamian; Fatemeh Hajmoradi
Abstract
The genus Onobrychis belongs to family Fabaceae and has about 130 species throughout the world.
Onobrychis sect. Hymenobrychis with nearly 14 species in Iran is one of the important sections of the genus.
The chromosome number and meiotic behaviour were studied in two populations of Onobrychis chorassanica
belonging ...
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The genus Onobrychis belongs to family Fabaceae and has about 130 species throughout the world.
Onobrychis sect. Hymenobrychis with nearly 14 species in Iran is one of the important sections of the genus.
The chromosome number and meiotic behaviour were studied in two populations of Onobrychis chorassanica
belonging to this section native to Iran. This report is the first cytogenetic analysis of this taxon. Both
populations are diploid and possess 2n = 2x = 14 chromosome number, consistent with the proposed base
number of x = 7. Although these taxa displayed regular bivalent pairing and chromosome segregation at
meiosis, some meiotic abnormalities were observed. The meiotic abnormalities included varied degrees of
chromosome stickiness including B-chromosome, cytomixis, asynchronous nucleus, fragmented chromosomes,
occurrence of laggard chromosomes, chromosome bridges, desynapsis, micronucleous and tripolar cells.
