Plant regeneration via somatic embryogenesis and organogenesis in Verbascum speciosum Schard

Document Type : Research Articles

Authors

Roya Karamian

Fatemeh Ghasemlou

Bu-Ali Sina University, Hamedan, Iran

https://doi.org/10.22067/jcmr.v4i2.22591

Abstract

Plant regeneration was achieved in Verbascum speciosum Schard. via organogenesis and somatic
embryogenesis by culture of mature embryo explants. Two types of calli, embryogenic and non-embryogenic,
were induced from mature embryo explants on Murashige and Skoog (MS) medium supplemented with
different concentrations of benzyl adenine (BA) and α-naphthalene acetic acid (NAA). In order to further
proliferate the somatic embryoids, the yellow and friable embryogenic calli were transferred on MS medium
containing 0.5 mg-1 charchol and 0.1 or 1 mg-1 2,4-dichlorophenoxy acetic acid (2,4-D) or into MS medium
containing 60 g-1 sucrose, 50 mgl-1 casein hydrolysate (CH), 0.5 mg-1 kinetin (Kin), 5 mg-1 2,4-D and 0.5 mg-1
charchol. Among the 3 tested media, MS medium containing 0.1 mg-1 2,4-D and 0.5 mg-1 charchol was more
effective for proliferation of embryonic calli. Somatic embryos were transferred to hormone free MS medium
for maturation and shoot regeneration. In addition, shoots and roots regenerated from non-embryogenic calli in
hormone free MS medium or containing NAA and BA. Shoot buds were obtained from non-embryogenic calli
and they were transferred to MS medium supplemented with 1 mg-1 BA or Kin for further growth and
multiplication. Regenerated plants then were potted and maintained in the greenhouse.

Keywords

embryogenic calli

shoot and root regeneration

Verbascum speciosum

20.1001.1.20089147.2012.4.2.6.8

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