Investigating the Relationship between the rs1800872 Polymorphism of the Interleukin 10 Gene Promoter Region and Breast Cancer in Kerman Province

Articles in Press

Document Type : Research Articles

Authors

Roya Rostaminiya

Masoumeh Hajirezaei

Department of Microbiology, Faculty of Science, Islamic Azad University, Kerman, Iran

10.22067/jcmr.2025.92932.1112

Abstract

Interleukin 10 (IL-10) is a crucial anti-inflammatory cytokine that plays a significant role in immune regulation and is overexpressed in various cancers, including breast cancer. IL-10 is known to modulate immune responses by suppressing the production of inflammatory cytokines, thereby influencing tumor progression and immune evasion. The expression of IL-10 is primarily regulated by single-nucleotide polymorphisms in its promoter region, which can influence its transcriptional activity and, consequently, cancer susceptibility. One such polymorphism, rs1800872, has been extensively investigated for its potential association with breast cancer risk and disease progression in different populations. The objective of this study was to explore the relationship between the rs1800872 polymorphism of the IL-10 gene and breast cancer in women from Kerman province, Iran. In this case-control study, the sample size was calculated using the two-proportion formula and G*Power software, with 80% power and a 5% significance level. Due to limited sample availability, 120 healthy controls and 170 breast cancer patients were included, which was considered sufficient for statistical analysis. Genomic DNA was extracted from these samples, and genotyping for the rs1800872 polymorphism was performed using the Allele-Specific Polymerase Chain Reaction (AS-PCR) method. Statistical analysis using SPSS version 26 and the Chi-square test showed a significant difference in genotype and allele distribution between breast cancer patients and healthy controls (P = 0.001). The AA genotype was more frequent in patients (24.1% vs. 12.5%), with an odds ratio (OR) of 2.66 (95% CI: 1.35–5.22). The A allele was also more common in patients (40.3% vs. 26.25%) with an OR of 1.90 (95% CI: 1.33–2.72), indicating a strong association of the A allele with increased breast cancer risk in the studied population.

Keywords

Breast Cancer

Cytokine

Interleukin 10

rs1800872 Polymorphism

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