Afra S., Koch M., Żur-Pińska J., Dolatshahi M., Bahrami A. R., Sayed J. E., et al. (2024) Chitosan/nanohydroxyapatite/hydroxyethyl-cellulose-based printable formulations for local alendronate drug delivery in osteoporosis treatment. Carbohydrate Polymer Technologies and Applications 7:100418.
Agarwal T., Chiesa I., Costantini M., Lopamarda A., Tirelli M. C., Borra O. P., et al. (2023) Chitosan and its derivatives in 3D/4D (bio) printing for tissue engineering and drug delivery applications. International Journal of Biological Macromolecules 246:125669.
Agnihotri S. A., Mallikarjuna N. N. and Aminabhavi T. M. (2004) Recent advances on chitosan-based micro-and nanoparticles in drug delivery. Journal of Controlled Release 100:5–28.
Ahern B., Parvizi J., Boston R. and Schaer T. (2009) Preclinical animal models in single site cartilage defect testing: a systematic review. Osteoarthritis and Cartilage 17:705–713.
Akmeşe R., Ertan M. B. and Kocaoğlu H. (2020) Comparison of chitosan-based liquid scaffold and hyaluronic acid–based soft scaffold for treatment of talus osteochondral lesions. Foot & Ankle International 41:1240–1248.
Akmeşe R., Özbek E. A., Kocaoğlu H., Ertan M. B., İnanç İ. and Erdemli E. (2022) Comparison of all arthroscopic implantation of chitosan-based liquid scaffold and hyaluronan-based soft scaffold in the treatment of condylar osteochondral lesions in the knee. The Journal of Knee Surgery 35:222–230.
Alanazi B. N., Ahmed H. A., Alharbi N. S., Ebrahim N. A. and Soliman S. M. (2025) Exploring 4D printing of smart materials for regenerative medicine applications. Rsc Advances 15:32155–32171.
Aranaz I., Alcántara A. R., Civera M. C., Arias C., Elorza B., Heras Caballero, et al. (2021) Chitosan: An overview of its properties and applications. Polymers 13:3256.
Asghari Sana F., Çapkın Yurtsever M., Kaynak Bayrak G., Tunçay E. Ö., Kiremitçi A. S. and Gümüşderelioğlu M. (2017) Spreading, proliferation and differentiation of human dental pulp stem cells on chitosan scaffolds immobilized with RGD or fibronectin. Cytotechnology 69:617–630.
Aytekin K. and Esenyel C. Z. (2021) Comparing BST-CarGel® with hyalofast for the treatment of hyaline cartilage defects. European Archives of Medical Research 37.
Berger J., Reist M., Mayer J., Felt O. and Gurny R. (2004) Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications. European Journal of Pharmaceutics and Biopharmaceutics 57:35–52.
Bernabé P., Peniche C. and Argüelles-Monal W. (2005) Swelling behavior of chitosan/pectin polyelectrolyte complex membranes. Effect of thermal cross-linking. Polymer Bulletin 55:367–375.
Bez M., Foiret J., Shapiro G., Pelled G., Ferrara K. W. and Gazit D. (2019) Nonviral ultrasound-mediated gene delivery in small and large animal models. Nature Protocols 14:1015–1026.
Bhattarai N., Edmondson D., Veiseh O., Matsen F. A. and Zhang M. (2005) Electrospun chitosan-based nanofibers and their cellular compatibility. Biomaterials 26:6176–6184.
Bhattarai N., Gunn J. and Zhang M. (2010) Chitosan-based hydrogels for controlled, localized drug delivery. Advanced Drug Delivery Reviews 62:83–99.
Bohlouli N., Mirzaei E., Ghanbari H., Sorkhabadi S. M. R. and Faridi-Majid R. (2018) Reinforcing mechanical strength of electrospun chitosan nanofibrous scaffold using cellulose nanofibers. Journal of Nano Research 52:71–79.
Bölgen N. and Vaseashta A. (2016) Nanofibers for tissue engineering and regenerative medicine. In 3rd International Conference on Nanotechnologies and Biomedical Engineering: ICNBME-2015, September 23-26, 2015, Chisinau, Republic of Moldova. Springer. 319–322.
Bowman K. and Leong K. W. (2006) Chitosan nanoparticles for oral drug and gene delivery. International Journal of Nanomedicine 1:117–128.
Cao Y., Tan Y. F., Wong Y. S., Liew M. W. J. and Venkatraman S. (2019) Recent advances in chitosan-based carriers for gene delivery. Marine Drugs 17:381.
Catoira M. C., Fusaro L., Di Francesco D., Ramella M. and Boccafoschi F. (2019) Overview of natural hydrogels for regenerative medicine applications. Journal of Materials Science: Materials in Medicine 30:115.
Chang J., Wang W., Zhang H., Hu Y., Wang M. and Yin Z. (2013) The dual role of autophagy in chondrocyte responses in the pathogenesis of articular cartilage degeneration in osteoarthritis. International Journal of Molecular Medicine 32:1311–1318.
Chenite A., Chaput C., Wang D., Combes C., Buschmann M. D., Hoemann C, et al. (2000) Novel injectable neutral solutions of chitosan form biodegradable gels in situ. Biomaterials 21:2155–2161.
Chevrier A., Hoemann C. D., Sun J. and Buschmann M. D. (2007) Chitosan–glycerol phosphate/blood implants increase cell recruitment, transient vascularization and subchondral bone remodeling in drilled cartilage defects. Osteoarthritis and Cartilage 15:316–327.
Chiang H. and Jiang C. C. (2009) Repair of articular cartilage defects: review and perspectives. Journal of the Formosan Medical Association 108:87–101.
Chicea D. and Nicolae-Maranciuc A. (2024) A review of chitosan-based materials for biomedical, food, and water treatment applications. Materials 17:5770.
Chimene D., Kaunas R. and Gaharwar A. K. (2020) Hydrogel bioink reinforcement for additive manufacturing: A focused review of emerging strategies. Advanced Materials 32:1902026.
Chonanant C., Chancharoen P., Kiatkulanusorn S., Luangpon N., Klarod K., Surakul P., et al. (2024) Biocomposite scaffolds based on chitosan extraction from shrimp shell waste for cartilage tissue engineering application. ACS Omega 9:39419–39429.
Chu C. R., Szczodry M. and Bruno S. (2010) Animal models for cartilage regeneration and repair. Tissue Engineering Part B: Reviews 16:105–115.
Chuan D., Jin T., Fan R., Zhou L. and Guo G. (2019) Chitosan for gene delivery: Methods for improvement and applications. Advances in Colloid and Interface Science 268:25–38.
Croisier F. and Jérôme C. (2013) Chitosan-based biomaterials for tissue engineering. European polymer journal 49:780–792.
Cui P.-F., Qi L.-Y., Wang Y., Yu R.-Y., He Y.-J., Xing L, et al. (2019) Dex-Aco coating simultaneously increase the biocompatibility and transfection efficiency of cationic polymeric gene vectors. Journal of Controlled Release 303:253–262.
Danielsen S., Strand S., de Lange Davies C. and Stokke B. T. (2005) Glycosaminoglycan destabilization of DNA–chitosan polyplexes for gene delivery depends on chitosan chain length and GAG properties. Biochimica et Biophysica Acta (BBA)-General Subjects 1721:44–54.
Das S. and Basu B. (2019) An overview of hydrogel-based bioinks for 3D bioprinting of soft tissues. Journal of the Indian Institute of Science 99:405–428.
Delvaux M., Chevrier A., Sim S., Quenneville É., Hurtig M. B., De Crescenzo G., et al. (2025) Chitosan/Platelet‐Rich plasma implants in an ovine arthroscopic model of meniscus repair. Journal of Orthopaedic Research®.
Demirtaş T. T., Irmak G. and Gümüşderelioğlu M. (2017) A bioprintable form of chitosan hydrogel for bone tissue engineering. Biofabrication 9:035003.
Derakhshanfar S., Mbeleck R., Xu K., Zhang X., Zhong W. and Xing M. (2018) 3D bioprinting for biomedical devices and tissue engineering: A review of recent trends and advances. Bioactive Materials 3:144–156.
Ding Y., Zhu J., Guan X., Li C., Cai P., Lu C., et al. (2025) Photocrosslinkable hydrogel of kartogenin functionalized chitosan methacrylate preserves chondrogenesis. International Journal of Biological Macromolecules:146378.
Dover S. (2007) Bone and cartilage engineering, U. Meyer, HP Weismann, Springer 2006. ISBN: 103-540-25347-5. Churchill Livingstone.
Faccendini A., Ruggeri M., Miele D., Rossi S., Bonferoni M. C., Aguzzi C., et al. (2020) Norfloxacin-loaded electrospun scaffolds: Montmorillonite nanocomposite vs. free drug. Pharmaceutics 12:325.
Fan J., Lee C.-S., Kim S., Zhang X., Pi-Anfruns J., Guo M., et al. (2021) Trb3 controls mesenchymal stem cell lineage fate and enhances bone regeneration by scaffold-mediated local gene delivery. Biomaterials 264:120445.
Frisbie D., Cross M. and McIlwraith C. (2006) A comparative study of articular cartilage thickness in the stifle of animal species used in human pre-clinical studies compared to articular cartilage thickness in the human knee. Veterinary and Comparative Orthopaedics and Traumatology 19:142–146.
García-Couce J., Tomás M., Fuentes G., Que I., Almirall A. and Cruz L. J. (2022) Chitosan/Pluronic F127 thermosensitive hydrogel as an injectable dexamethasone delivery carrier. Gels 8:44.
Ghassemi T., Saghatoleslami N., Mahdavi-Shahri N., Matin M. M., Gheshlaghi R. and Moradi A. (2019) A comparison study of different decellularization treatments on bovine articular cartilage. Journal of Tissue Engineering and Regenerative Medicine 13(10): 1861-1871.
Gilbert S. J. and Blain E. J. (2018) Cartilage mechanobiology: How chondrocytes respond to mechanical load. In Mechanobiology in Health and Disease. Elsevier. 99–126.
Gupta P., Sharma S., Jabin S. and Jadoun S. (2024) Chitosan nanocomposite for tissue engineering and regenerative medicine: a review. International Journal of Biological Macromolecules 254:127660.
Gupte M. J., Swanson W. B., Hu J., Jin X., Ma H., Zhang Z., et al. (2018) Pore size directs bone marrow stromal cell fate and tissue regeneration in nanofibrous macroporous scaffolds by mediating vascularization. Acta Biomaterialia 82:1–11.
Hall B. K. (2005) Bones and cartilage: developmental and evolutionary skeletal biology. Elsevier.
Hejazi F., Bagheri‐Khoulenjani S., Olov N., Zeini D., Solouk A. and Mirzadeh H. (2021) Fabrication of nanocomposite/nanofibrous functionally graded biomimetic scaffolds for osteochondral tissue regeneration. Journal of Biomedical Materials Research Part A 109:1657–1669.
Henson F. M., Getgood A. M., Caborn D. M., McIlwraith C. W. and Rushton N. (2012) Effect of a solution of hyaluronic acid–chondroitin sulfate–N-acetyl glucosamine on the repair response of cartilage to single-impact load damage. American Journal of Veterinary Research 73:306–312.
Hospodiuk M., Dey M., Sosnoski D. and Ozbolat I. T. (2017) The bioink: A comprehensive review on bioprintable materials. Biotechnology Advances 35:217–239.
Hou S.-M., Hou C.-H. and Liu J.-F. (2017) CX3CL1 promotes MMP-3 production via the CX3CR1, c-Raf, MEK, ERK, and NF-κB signaling pathway in osteoarthritis synovial fibroblasts. Arthritis Research & Therapy 19:282.
Huang J., Fu H., Wang Z., Meng Q., Liu S., Wang H., et al. (2016) BMSCs-laden gelatin/sodium alginate/carboxymethyl chitosan hydrogel for 3D bioprinting. Rsc Advances 6:108423–108430.
Intini C., Lemoine M., Hodgkinson T., Casey S., Gleeson J. P. and O'Brien F. J. (2022) A highly porous type II collagen containing scaffold for the treatment of cartilage defects enhances MSC chondrogenesis and early cartilaginous matrix deposition. Biomaterials Science 10:970–983.
Jahani A., Nourbakhsh M. S., Moradi A., Mohammadi M. and Tayebi L. (2025) Incorporating insulin into alginate-chitosan 3D-printed scaffolds: A comprehensive study on structure, mechanics, and biocompatibility for cartilage tissue engineering. Carbohydrate Polymer Technologies and Applications 9:100696.
Jelodari S., Ebrahimi Sadrabadi A., Zarei F., Jahangir S., Azami M., Sheykhhasan M., et al. (2022) New insights into cartilage tissue engineering: improvement of tissue‐scaffold integration to enhance cartilage regeneration. BioMed Research International 2022:7638245.
Jeyaraman M., Jeyaraman N., Ramasubramanian S. and Nallakumarasamy A. (2025) Advancements in biomaterials for cartilage tissue engineering: Challenges and future directions. In Biomaterials in Orthopaedics & Trauma: Current Status and Future Trends in Revolutionizing Patient Care. Springer. 147–168.
Jiang H.-L., Islam M. A., Xing L., Firdous J., Cao W., He Y.-J., et al. (2017) Degradable polyethylenimine-based gene carriers for cancer therapy. Polymeric Gene Delivery Systems:113–148.
Karayianni M., Sentoukas T., Skandalis A., Pippa N. and Pispas S. (2023) Chitosan-based nanoparticles for nucleic acid delivery: technological aspects, applications, and future perspectives. Pharmaceutics 15:1849.
Khatami F., Matin M. M., Danesh N. M., Bahrami A. R., Abnous K. and Taghdisi S. M. (2021) Targeted delivery system using silica nanoparticles coated with chitosan and AS1411 for combination therapy of doxorubicin and antimiR-21. Carbohydrate Polymers 266:118111.
Kilian D., Sembdner P., Bretschneider H., Ahlfeld T., Mika L., Lützner J., et al. (2021) 3D printing of patient-specific implants for osteochondral defects: Workflow for an MRI-guided zonal design. Bio-Design and Manufacturing 4:818–832.
Kim M. A., Shin S. R., Kim H. J., Lee J. S. and Lee C.-M. (2022) Chemo-photothermal therapeutic effect of chitosan-gelatin hydrogels containing methotrexate and melanin on a collagen-induced arthritis mouse model. International Journal of Biological Macromolecules 218:1013–1020.
Knowlton S., Yenilmez B., Anand S. and Tasoglu S. (2017) Photocrosslinking-based bioprinting: Examining crosslinking schemes. Bioprinting 5:10–18.
Kohut A., Voronov A. and Peukert W. (2007) An effective way to stabilize colloidal particles dispersed in polar and nonpolar media. Langmuir 23:504–508.
Leedy M. R., Martin H. J., Norowski P. A., Jennings J. A., Haggard W. O. and Bumgardner J. D. (2011) Use of chitosan as a bioactive implant coating for bone-implant applications. In Chitosan for Biomaterials II. Springer. 129–165.
Levengood S. K. L. and Zhang M. (2014) Chitosan-based scaffolds for bone tissue engineering. Journal of Materials Chemistry B 2:3161–3184.
Li M., Li F., Xu J., Zhu L., Xiang J., Zhu C., et al. (2025) Bioadhesive chitosan hydrogel with dynamic covalent bonds and sustained kartogenin release for endogenous cartilage regeneration. Frontiers in Bioengineering and Biotechnology 13:1606726.
Li P., Fu L., Liao Z., Peng Y., Ning C., Gao C., et al. (2021) Chitosan hydrogel/3D-printed poly (ε‐caprolactone) hybrid scaffold containing synovial mesenchymal stem cells for cartilage regeneration based on tetrahedral framework nucleic acid recruitment. Biomaterials 278:121131.
Liang H., Chen X., Jin R., Ke B., Barz M., Ai H. and Nie Y. (2020) Integration of indocyanine green analogs as near‐infrared fluorescent carrier for precise imaging‐guided gene delivery. Small 16:1906538.
Liang R., Li Z., Xu Q., Wang S., Chen D., Gui P., et al. (2025) Immunomodulatory exosomes loaded chitosan methacryloyl hydrogel combined with pro-chondrogenic microspheres for promoting articular cartilage regeneration. Materials & Design 253:113876.
Lima E. G., Bian L., Ng K. W., Mauck R. L., Byers B. A., Tuan R. S., et al. (2007) The beneficial effect of delayed compressive loading on tissue-engineered cartilage constructs cultured with TGF-β3. Osteoarthritis and Cartilage 15:1025–1033.
Lin L., Wang Y., Wang L., Pan J., Xu Y., Li S., et al. (2020) Injectable microfluidic hydrogel microspheres based on chitosan and poly (ethylene glycol) diacrylate (PEGDA) as chondrocyte carriers. Royal Society of Chemistry Advances 10:39662–39672.
Lind M., Larsen A., Clausen C., Osther K. and Everland H. (2008) Cartilage repair with chondrocytes in fibrin hydrogel and MPEG polylactide scaffold: An in vivo study in goats. Knee Surgery, Sports Traumatology, Arthroscopy 16:690–698.
Liu X., Jin X. and Ma P. X. (2011) Nanofibrous hollow microspheres self-assembled from star-shaped polymers as injectable cell carriers for knee repair. Nature Materials 10:398–406.
Lu H., Lv L., Dai Y., Wu G., Zhao H. and Zhang F. (2013) Porous chitosan scaffolds with embedded hyaluronic acid/chitosan/plasmid-DNA nanoparticles encoding TGF-β1 induce DNA controlled release, transfected chondrocytes, and promoted cell proliferation. PloS One 8:e69950.
Ma S., Zhang L., Wu Y., Huang W., Liu F., Li M., Fan Y., Xia H., Wang X. and Li X. (2025) Glucosamine sulfate-loaded nanofiber reinforced carboxymethyl chitosan sponge for articular cartilage restoration. Journal of Colloid and Interface Science 677:632–644.
Madihally S. V. and Matthew H. W. (1999) Porous chitosan scaffolds for tissue engineering. Biomaterials 20:1133–1142.
Madry H. and Cucchiarini M. (2014) Tissue-engineering strategies to repair joint tissue in osteoarthritis: Nonviral gene-transfer approaches. Current Rheumatology Reports 16:450.
Mahapatra C., Kim J.-J., Lee J.-H., Jin G.-Z., Knowles J. C. and Kim H.-W. (2019) Differential chondro-and osteo-stimulation in three-dimensional porous scaffolds with different topological surfaces provides a design strategy for biphasic osteochondral engineering. Journal of Tissue Engineering 10:2041731419826433.
Maneechan W., Khumfu P., Charoensit P., Tuanchai A., Ross S., Ross G. M., et al. (2025) Bioactive hydrogel scaffolds integrating chitosan, silk fibroin, and Aloe vera extract for enhanced cartilage tissue regeneration. Polymers 17:1409.
Mars S. W., Davidson B. L., Moore K. H., Boardman B. M. and Peters G. M. (2024) Importance of primary and secondary hydrogen bonding interactions of polyols on the plasticization of chitosan. ACS Omega 9:41687–41695.
McCullen S. D., Autefage H., Callanan A., Gentleman E. and Stevens M. M. (2012) Anisotropic fibrous scaffolds for articular cartilage regeneration. Tissue Engineering Part A 18:2073–2083.
Min Q., Tian D., Zhang Y., Wang C., Wan Y. and Wu J. (2022) Strong and elastic chitosan/silk fibroin hydrogels incorporated with growth-factor-loaded microspheres for cartilage tissue engineering. Biomimetics 7:41.
Miri Z., Haugen H. J., Loca D., Rossi F., Perale G., Moghanian A., et al. (2024) Review on the strategies to improve the mechanical strength of highly porous bone bioceramic scaffolds. Journal of the European Ceramic Society 44:23–42.
Mirmusavi M. H., Ahmadian M. and Karbasi S. (2022) Polycaprolactone-chitosan/multi-walled carbon nanotube: A highly strengthened electrospun nanocomposite scaffold for cartilage tissue engineering. International Journal of Biological Macromolecules 209:1801–1814.
Moghadam N. A., Bagheri F. and Eslaminejad M. B. (2022) Chondroitin sulfate modified chitosan nanoparticles as an efficient and targeted gene delivery vehicle to chondrocytes. Colloids and Surfaces B: Biointerfaces 219:112786.
Morris V. B., Nimbalkar S., Younesi M., McClellan P. and Akkus O. (2017) Mechanical properties, cytocompatibility and manufacturability of chitosan: PEGDA hybrid-gel scaffolds by stereolithography. Annals of Biomedical Engineering 45:286–296.
Murab S., Herold S., Hawk T., Snyder A., Espinal E. and Whitlock P. (2023) Advances in additive manufacturing of polycaprolactone based scaffolds for bone regeneration. Journal of Materials Chemistry B 11:7250–7279.
Murphy S. V. and Atala A. (2014) 3D bioprinting of tissues and organs. Nature Biotechnology 32:773–785.
Negm N. A., Hefni H. H., Abd-Elaal A. A., Badr E. A. and Abou Kana M. T. (2020) Advancement on modification of chitosan biopolymer and its potential applications. International Journal of Biological Macromolecules 152:681–702.
Naderi‐Meshkin H., Andreas K., Matin M. M., Sittinger M., Bidkhori H. R., Ahmadiankia N., et al. (2014) Chitosan‐based injectable hydrogel as a promising in situ forming scaffold for cartilage tissue engineering. Cell Biology International 38:72–84.
Neves S. C., Teixeira L. S. M., Moroni L., Reis R. L., Van Blitterswijk C. A., Alves N. M., et al. (2011) Chitosan/Poly (ɛ-caprolactone) blend scaffolds for cartilage repair. Biomaterials 32:1068–1079.
Ngo P.-K. T., Nguyen D. N., Nguyen H.-P., Tran T.-H. H., Nguyen Q.-N. D., Luu C. H., et al. (2024) Silk fibroin/chitosan/montmorillonite sponge dressing: Enhancing hemostasis, antimicrobial activity, and angiogenesis for advanced wound healing applications. International Journal of Biological Macromolecules 279:135329.
Nguyen M.-A., Wyatt H., Susser L., Geoffrion M., Rasheed A., Duchez A.-C., et al. (2019) Delivery of microRNAs by chitosan nanoparticles to functionally alter macrophage cholesterol efflux in vitro and in vivo. ACS Nano 13:6491–6505.
Nicolle L., Journot C. M. and Gerber-Lemaire S. (2021) Chitosan functionalization: Covalent and non-covalent interactions and their characterization. Polymers 13:4118.
Nikhil A. and Kumar A. (2022) Evaluating potential of tissue‐engineered cryogels and chondrocyte derived exosomes in articular cartilage repair. Biotechnology and Bioengineering 119:605–625.
Nour-Eldeen G., Abdel-Rasheed M., El-Rafei A. M., Azmy O. and El-Bassyouni G. T. (2020) Adipose tissue-derived mesenchymal stem cells and chitosan/poly (vinyl alcohol) nanofibrous scaffolds for cartilage tissue engineering. Cell Regeneration 9:7.
O'Reilly A. and Kelly D. J. (2017) A computational model of osteochondral defect repair following implantation of stem cell-laden multiphase scaffolds. Tissue Engineering Part A 23:30–42.
Obeidat W. M. and Lahlouh I. K. (2025) Chitosan nanoparticles: approaches to preparation, key properties, drug delivery systems, and developments in therapeutic efficacy. AAPS PharmSciTech 26:108.
Omigbodun F. T. (2025) A Review inside Innovation: AI and additive manufacturing for advanced bone scaffold design. Igmin Research 3:346–361.
Parak A., Pradeep P., du Toit L. C., Kumar P., Choonara Y. E. and Pillay V. (2019) Functionalizing bioinks for 3D bioprinting applications. Drug Discovery Today 24:198–205.
Paul S., Schrobback K., Tran P. A., Meinert C., Davern J. W., Weekes A., et al. (2023) Photo‐cross‐linkable, injectable, and highly adhesive GelMA‐glycol chitosan hydrogels for cartilage repair. Advanced Healthcare Materials 12:2302078.
Peng L., Zhou Y., Lu W., Zhu W., Li Y., Chen K., et al. (2019) Characterization of a novel polyvinyl alcohol/chitosan porous hydrogel combined with bone marrow mesenchymal stem cells and its application in articular cartilage repair. BMC Musculoskeletal Disorders 20:257.
Pereira R. F. and Bártolo P. J. (2015) 3D bioprinting of photocrosslinkable hydrogel constructs. Journal of Applied Polymer Science 132.
Qindeel M., Ahmed N., Khan G. M. and Rehman A. u. (2019) Ligand decorated chitosan as an advanced nanocarrier for targeted delivery: A critical review. Nanomedicine 14:1623–1642.
Rajendrakumar S. K., Uthaman S., Cho C. S. and Park I. K. (2017) Trigger-responsive gene transporters for anticancer therapy. Nanomaterials 7:120.
Rawojć K., Tadeusiewicz R. and Zych-Stodolak E. (2025) Advancements in chitosan-based scaffolds for chondrogenic differentiation and knee cartilage regeneration: Current trends and future perspectives. Bioengineering 12:740.
Rinaudo M. (2006) Chitin and chitosan: Properties and applications. Progress in Polymer Science 31:603–632.
Rodríguez-Merchán E. C. (2012) The treatment of cartilage defects in the knee joint: microfracture, mosaicplasty, and autologous chondrocyte implantation. Am J Orthop (Belle Mead NJ) 41:236–239.
Roy J. C., Salaün F., Giraud S., Ferri A., Chen G. and Guan J. (2017) Solubility of chitin: Solvents, solution behaviors and their related mechanisms. Solubility of Polysaccharides:109.
Saberi A., Khodaverdi E., Kamali H., Movaffagh J., Mohammadi M., Yari D., et al. (2024) Fabrication and characterization of biomimetic electrospun cartilage decellularized matrix (CDM)/chitosan nanofiber hybrid for tissue engineering applications: Box-Behnken design for optimization. Journal of Polymers and the Environment 32:1573–1592.
Sahai N., GOGOI M. and ROHILLA P. K. (2017) Development of chitosan porous tissue scaffolds using computer aided tissue engineering for cartilage regeneration. Asian Chitin Journal 13:9-14
Sahranavard M., Zamanian A., Ghorbani F. and Shahrezaee M. H. (2020) A critical review on three dimensional-printed chitosan hydrogels for development of tissue engineering. Bioprinting 17:e00063.
Sancho-Tello M., Martorell S., Mata Roig M., Milián L., Gámiz-González M., Gomez Ribelles J. L., et al. (2017) Human platelet-rich plasma improves the nesting and differentiation of human chondrocytes cultured in stabilized porous chitosan scaffolds. Journal of Tissue Engineering 8:2041731417697545.
Schagemann J., Paul S., Casper M., Rohwedel J., Kramer J., Kaps C., et al. (2013) Chondrogenic differentiation of bone marrow‐derived mesenchymal stromal cells via biomimetic and bioactive poly‐ε‐caprolactone scaffolds. Journal of Biomedical Materials Research Part A 101:1620–1628.
Sechriest V. F., Miao Y. J., Niyibizi C., Westerhausen–Larson A., Matthew H. W., Evans C. H., et al. (2000) GAG‐augmented polysaccharide hydrogel: A novel biocompatible and biodegradable material to support chondrogenesis. Journal of Biomedical Materials Research: An Official Journal of The Society for Biomaterials and The Japanese Society for Biomaterials 49:534–541.
Sencadas V., Correia D. M., Ribeiro C., Moreira S., Botelho G., Ribelles J. G., et al. (2012) Physical-chemical properties of cross-linked chitosan electrospun fiber mats. Polymer Testing 31:1062–1069.
Shafiee A., Soleimani M., Chamheidari G. A., Seyedjafari E., Dodel M., Atashi A., et al. (2011) Electrospun nanofiber‐based regeneration of cartilage enhanced by mesenchymal stem cells. Journal of Biomedical Materials Research Part A 99:467–478.
Shen Y., Xu Y., Yi B., Wang X., Tang H., Chen C., et al. (2021) Engineering a highly biomimetic chitosan-based cartilage scaffold by using short fibers and a cartilage-decellularized matrix. Biomacromolecules 22:2284–2297.
Sheng W., Qin H., Wang T., Zhao J., Fang C., Zhang P., et al. (2023) Advanced phosphocreatine-grafted chitosan hydrogel promote wound healing by macrophage modulation. Frontiers in Bioengineering and Biotechnology 11:1199939.
Shih P.-Y., Liao Y.-T., Tseng Y.-K., Deng F.-S. and Lin C.-H. (2019) A potential antifungal effect of chitosan against Candida albicans is mediated via the inhibition of SAGA complex component expression and the subsequent alteration of cell surface integrity. Frontiers in Microbiology 10:602.
Shive M. S., Hoemann C. D., Restrepo A., Hurtig M. B., Duval N., Ranger P., et al. (2006) BST-CarGel: In situ chondroinduction for cartilage repair. Operative Techniques in Orthopaedics 16:271–278.
Shoueir K. R., El-Desouky N., Rashad M. M., Ahmed M., Janowska I. and El-Kemary M. (2021) Chitosan based-nanoparticles and nanocapsules: Overview, physicochemical features, applications of a nanofibrous scaffold, and bioprinting. International Journal of Biological Macromolecules 167:1176–1197.
Silvestro I., Sergi R., d'Abusco A. S., Mariano A., Martinelli A., Piozzi A., et al. (2021) Chitosan scaffolds with enhanced mechanical strength and elastic response by combination of freeze gelation, photo-crosslinking and freeze-drying. Carbohydrate Polymers 267:118156.
Smaida R., Pijnenburg L., Irusta S., Himawan E., Mendoza G., Harmouch E., et al. (2020) Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration. Materials 13:3087.
Souza-Silva L. C., Cristina Santi Martignago C., Garcia Motta H., Bonifacio M., Regina Avanzi I., Assis L., et al. (2024) A Review of cartilage defect treatments using chitosan hydrogels in experimental animal models. Current Pharmaceutical Biotechnology 25:1058–1072.
Stanish W. D., McCormack R., Forriol F., Mohtadi N., Pelet S., Desnoyers J., et al. (2013) Novel scaffold-based BST-CarGel treatment results in superior cartilage repair compared with microfracture in a randomized controlled trial. Journal of Bone and Joint Surgery 95:1640–1650.
Steinmetz J. D., Culbreth G. T., Haile L. M., Rafferty Q., Lo J., Fukutaki K. G., et al. (2023) Global, regional, and national burden of osteoarthritis, 1990–2020 and projections to 2050: A systematic analysis for the Global Burden of Disease Study 2021. The Lancet Rheumatology 5:e508–e522.
Suh J.-K. F. and Matthew H. W. (2000) Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials 21:2589–2598.
Taghizadeh M., Taghizadeh A., Yazdi M. K., Zarrintaj P., Stadler F. J., Ramsey J. D., et al. (2022) Chitosan-based inks for 3D printing and bioprinting. Green Chemistry 24:62–101.
Talouki P. Y., Tamimi R. and Rudi S. G. (2025) A comprehensive review of curcumin-based scaffolds in cartilage tissue engineering. Stem Cell Research & Therapy 16:528.
Tamburaci S., Cecen B., Ustun O., Ergur B. U., Havitcioglu H. and Tihminlioglu F. (2019) Production and characterization of a novel bilayer nanocomposite scaffold composed of chitosan/Si-nHap and zein/POSS structures for osteochondral tissue regeneration. ACS Applied Bio Materials 2:1440–1455.
Tian B. and Liu J. (2023) Smart stimuli-responsive chitosan hydrogel for drug delivery: A review. International Journal of Biological Macromolecules 235:123902.
Tirella A., Orsini A., Vozzi G. and Ahluwalia A. (2009) A phase diagram for microfabrication of geometrically controlled hydrogel scaffolds. Biofabrication 1:045002.
Valentino A., Conte R., De Luca I., Di Cristo F., Peluso G., Bosetti M., et al. (2022) Thermo-responsive gel containing hydroxytyrosol-chitosan nanoparticles (Hyt@ tgel) counteracts the increase of osteoarthritis biomarkers in human chondrocytes. Antioxidants 11:1210.
Van Der Kraan P. M. and Van Den Berg W. B. (2007) Osteophytes: Relevance and biology. Osteoarthritis and Cartilage 15:237–244.
Wan J., Jiang J., Yu X., Zhou J., Wang Y., Fu S., et al. (2025) Injectable biomimetic hydrogel based on modified chitosan and silk fibroin with decellularized cartilage extracellular matrix for cartilage repair and regeneration. International Journal of Biological Macromolecules 298:140058.
Wang C., Zhao W., Ma N., Chen L., Lu J., Liu S., et al. (2025) Chitosan/Gelatin/Carrageenan as a composite scaffolding material for cartilage tissue engineering. Journal of Medical and Biological Engineering:1–11.
Wang J., Zhang L., Zhu J., Gu J., Wang X. and Tao H. (2022) Hyaluronic acid modified curcumin-loaded chitosan nanoparticles inhibit chondrocyte apoptosis to attenuate osteoarthritis via upregulation of activator protein 1 and RUNX family transcription factor 2. Journal of Biomedical Nanotechnology 18:144–157.
Wang P., Hu J. and Ma P. X. (2009) The engineering of patient-specific, anatomically shaped, digits. Biomaterials 30:2735–2740.
Wang W., Meng Q., Li Q., Liu J., Zhou M., Jin Z., et al. (2020) Chitosan derivatives and their application in biomedicine. International Journal of Molecular Sciences 21:487.
Wang X., Ao Q., Tian X., Fan J., Wei Y., Hou W., et al. (2016) 3D bioprinting technologies for hard tissue and organ engineering. Materials 9:802.
Wu J., Chen Q., Deng C., Xu B., Zhang Z., Yang Y., et al. (2020) Exquisite design of injectable hydrogels in cartilage repair. Theranostics 10:9843.
Wu Q., Therriault D. and Heuzey M.-C. (2018) Processing and properties of chitosan inks for 3D printing of hydrogel microstructures. ACS Biomaterials Science & Engineering 4:2643–2652.
Xu T., Zhao W., Zhu J.-M., Albanna M. Z., Yoo J. J. and Atala A. (2013) Complex heterogeneous tissue constructs containing multiple cell types prepared by inkjet printing technology. Biomaterials 34:130–139.
Yang K., Wu Z., Zhang K., Weir M. D., Xu H. H., Cheng L., et al. (2024) Unlocking the potential of stimuli-responsive biomaterials for bone regeneration. Frontiers in Pharmacology 15:1437457.
Yu X., Wang Y., Liu X., Ge Y. and Zhang S. (2021) Ursolic acid loaded-mesoporous hydroxylapatite/chitosan therapeutic scaffolds regulate bone regeneration ability by promoting the M2-type polarization of macrophages. International Journal of Nanomedicine:5301–5315.
Yuan Z., Lyu Z., Zhang W., Zhang J. and Wang Y. (2022) Porous bioactive prosthesis with chitosan/mesoporous silica nanoparticles microspheres sequentially and sustainedly releasing platelet-derived growth factor-BB and kartogenin: a new treatment strategy for osteoarticular lesions. Frontiers in Bioengineering and Biotechnology 10:839120.
Yurtsever M. C., Akdere Ö. E. and Gümüşderelioğlu M. (2022) Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. Turkish Journal of Biology 46:475–487.
Zein N., Harmouch E., Lutz J.-C., Fernandez De Grado G., Kuchler-Bopp S., Clauss F., et al. (2019) Polymer-based instructive scaffolds for endodontic regeneration. Materials 12:2347.
Zhang K., Liu Y., Zhao Z., Shi X., Zhang R., He Y., et al. (2024) Magnesium-doped nano-hydroxyapatite/polyvinyl alcohol/chitosan composite hydrogel: Preparation and characterization. International Journal of Nanomedicine:651–671.
Zhang X., Yu C., Zhang C., Tang T. and Dai K. (2006) Direct chitosan-mediated gene delivery to the rabbit knee joints in vitro and in vivo. Biochemical and Biophysical Research Communications 341:202–208.
Zhang Y. S., Arneri A., Bersini S., Shin S.-R., Zhu K., Goli-Malekabadi Z., et al. (2016) Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip. Biomaterials 110:45–59.
Zhang Y. S., Yue K., Aleman J., Mollazadeh-Moghaddam K., Bakht S. M., Yang J., et al. (2017) 3D bioprinting for tissue and organ fabrication. Annals of Biomedical Engineering 45:148–163.
Zhang Z., Lin S., Yan Y., You X. and Ye H. (2021) Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering. Journal of Biomaterials Science, Polymer Edition 32:2402–2422.
Zheng B., Xing J., Tang X., He Z., Tang Q., Liu S., et al. (2025) Tailored surface topographical scaffolds: A breakthrough in osteoarthritic cartilage and subchondral bone defect repair. Journal of Materials Chemistry B 13(29):8639-8652.
Zhou T., Cavalcante R. C., Ge C., Franceschi R. T. and Ma P. X. (2025) Synthetic helical peptides on nanofibers to activate cell-surface receptors and synergistically enhance critical-sized bone defect regeneration. Bioactive Materials 43:98–113.
Zhou Y., Gao H. L., Shen L. L., Pan Z., Mao L. B., Wu T., et al. (2015) Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering. Nanoscale 8:309–317.
