Document Type : Research Articles
Authors
Gene Bank of Research Institute of Forests & Rangelands, Agricultural Research, Education and Extension Organization, Tehran, Iran
Abstract
Chamomile (Anthemis L.), an important medicinal plant belonging to the Asteraceae family, is widely distributed in Iran and other parts of the world, with 175 species. We can study chromosome characteristics such as chromosome numbers and karyotype characteristics to realize genetic variation within and between species and their populations. In the present study, we examined the details of the chromosome number and karyological characteristics analysis of 25 populations representing four Anthemis species (A. altissima, A. hausssknechtii, A. trimfettii, and A. pseudocotula) from geographically isolated regions of Iran. Specific staining method and microscopic observation showed somatic chromosome number 2n=18 for all populations, which were confirmed by the previous data. Analysis of the karyotype formula showed a dominance of metacentric chromosomes in almost all of them. The largest chromosome and genome length belonged to two populations of A. pseudocotula species. Populations of A. pseudocotula have chromosome size, and populations of A. haussknechtii have morphology chromosome variation. A cluster analysis of the tested accessions, at 11.19 genetic distance, created three main groups that showed the similarity of members of each group. Additionally, the level of symmetric karyotypes estimated by karyotype characters and the role of each trait in the variation of species and their populations are argued. Genetic variations were confirmed between diploid populations, for different karyotype characters. Observed variation mainly caused by the morphology of chromosomes, and that, its contribution was important in discriminating the populations. We hope these data will be used in future investigations as basic information for breeding and hybridization between species and their populations.
Keywords
Assis F.N.M., Souza B.C.Q., Medeiros-Neto E., Pinheiro F., Silva A.E.B. and Felix L.P. (2013) Karyology of the genus Epidendrum (Orchidaceae: Laeliinae) with emphasis on subgenus Amphiglottium and chromosome number variability in Epidendrum secundum. Botanical Journal of the Linnean Society 172(3): 329-344.
Chehregani-Rad A. and Mehanfar N. (2008) New Chromosome Counts in the Tribe Anthemideae (Astraceae) from Iran. Cytologia 73(2): 1-8.
Chehregani-Rad A., Mohsenzadeh F. and Saleh H. (2014) Investigations on chromosome variation in Achillea tenuifolia Lam and A. bieberestinii Afan (Asteraceae) from Hamedan and Kermanshah in the West of Iran. Journal of Cell and Molecular Research 6(1): 13-21.
Cruz-Sánchez F.F., Tapia-Campos E. and Barba-Gonzalez R. (2020) Importance of molecular cytogenetic markers in plant breeding. Acta Horticulture 1288: 167-174.
Funk V.A., Bayer R.J., Keeley S., Chan R., Watson L., Gemeinholzer B., Schilling E., Panero J.L., Baldwin B.G., Garcia-Jacas N., Susanna A. and Jansen R.K. (2005) Everywhere but Antarctica. Using a Super tree to understand the Diversity and Distribution of the Compositae. Biologiske Skrifter 55: 343-374.
Funk V.A., Susanna A., Stuessy T.F. and Robinson H. (2009) Classification of Compositae. In V. A. Funk, A. Susanna, T. Stuessy, and R. Bayer (Eds.) Systematics, evolution and biogeography of the Compositae (pp.171-189). Vienna: IAPT Press.
Garcia S, Panero J.L., Siroky J. and Kovarik A. (2010) Repeated reunions and splits feature the highly dynamic evolution of 5S and 35S ribosomal RNA genes (rDNA) in the Asteraceae family. BMC Plant Biology 10(176): 1-18.
Goula K., Touloumis K., Dimopoulos P. and Costantinidis T. (2022) A Morphometric and Karyological Study of the Anthemis macedonica Group (Asteraceae, Anthemideae) Reveals a New Species from Greece. Plants 11(3006): 1-22.
Huziwara Y. (1962) Karyotype analysis in some genera of Composite. VIII Further studies on the chromosome of Aster. American Journal of Botany 49: 116-119.
Inceer H. and Hayirlioglu-Ayaz S. (2007) Chromosome numbers in the tribe Anthemideae (Asteraceae) from North-East Anatolia. Botanical Journal of the Linnean Society 153, 203-211.
Javadi H., Alizadeh M.A., Emami H. and Salehi Shanjani P. (2013) Cytogenetices investigation in different populations of Anthemis tinctoria L. (Yellow chamomile) accessions from Iran. Chromosome Science 16: 43-49.
Javadi H. and Salehi Shanjani P. (2022) Comparative Cytogenetic within Achillea genus (Asteraceae) in Iran: I. chromosome number and morphology. Acta Biologica Szegediensis 66(1): 1-10.
Jeffrey C. (2007) Compositae: Introduction with Key to Tribes. In J.W. Kadereit and C. Jeffrey (Eds.) Families and Genera of Vascular Plants 8. Flowering Plants, Eudicots, Asterales (pp. 61-87). Berline: Springer- Verlag Press.
Kuzmanov B. (1978) Cytotaxonomic investigation of Bulgarian leguminous plants. In S. Kožuharov and B. Kuzmanov (Eds.) Evolution of Flowering Plants and Florogenesis (pp.11-71). Sofia: Academic Science Press.
Kuzmanov B. (1991) Evolutionary trends of Bulgarian Compositae. In S. Kožuharov and B. Kuzmanov (Eds.) Evolution of Flowering Plants and Florogenesis. Asteraceae, Brassicaceae, Poaceae, Cyperaceae (pp. 7-84). Sofia: Academic Science Press.
Levan A., Fredga K. and Sandberg A.A. (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52: 201-220.
Lynch M. and Conery J.S. (2003) The origins of genome complexity. Science 302: 1401-1404.
Mandel J.R., Dikow R.B., Siniscalchi C.M., Thapa R., Watson L.E. and Funk V.A. (2019) A fully resolved backbone phylogeny reveals numerous dispersals and explosive diversifications throughout the history of Asteraceae. Proceedings of the National Academy of Sciences 116(28): 14083-14088.
Markova M. (1989) Chromosome numbers of Bulgarian Angiospems. Phytologia 36: 67-68.
Moraes A.P, Leitch I.J. and Leitch A.R. (2012) Chromosome studies in Orchidaceae: Karyotype divergence in Neotropical genera in subtribe Maxillariinae. Botanical Journal of the Linnean Society Botanical Journal of the Linnean Society 170(1): 29-39.
Moraes A.P, Chinaglia M., Palma-Silva C. and Pinheiro F. (2013) Interploidy hybridization in sympatric zones: The formation of Epidendrum fulgens E. puniceoluteum hybrids (Epidendroideae, Orchidaceae). Ecology and Evolution 3(11): 3824-3837.
Moraes A.P., Simões A.O., Alayon D.I.O., Barros F. and Forni-Martins E.R. (2016) Detecting mechanisms of karyotype evolution in Heterotaxis (Orchidaceae). The Public Library of Science 11:e0165960
Mozaffarian V. (2006) A Dictionary of Iranian Plant Names. Tehran: Farhang Moaser Press.
Oberprieler C., Vogt R. and Watson L.E. (2007) Tribe Anthemideae. In J.W. Kadereit and C. Jeffrey (Eds.) The families and genera of vascular plants VIII (pp. 342-374). Berlin: Heidelberg Press.
Peruzzi L., Astuti G., Bartolucci F., Conti F. and Roma-Marzio F. (2016) Chromosome numbers for the Italian flora: 2. Italian. Botanist 2: 29-42.
Peruzzi L. and Eroğlu H.E. (2013) Karyotype asymmetry: again, how to measure and what to measure. Comparative Cytogenetics 7(1): 1-9.
Petrova A. and Vladimirov V. (2020) Chromosome atlas of the Bulgarian vascular plants. Phytologia Balcanica 26(2): 217-427.
Presti R.M.L., Oppolzer S. and Oberprieler C. (2010) A molecular phylogeny and a revised classification of the Mediterranean genus Anthemis s.l. (Compositae, Anthemideae) based on three molecular markers and micromorphological characters. Taxon 59: 1441-1456.
Rechinger K.H. (1986) Flora Iranica. No. 158 (pp. 1-44). Austria: Akademische Druck University press.
Reeves A. (2001) Micro Measure: A new computer program for the collection and analysis of cytogenetic data. Genome, 44(3): 439-443.
Romero Z.C. (1986) A new method for estimating karyotype asymmetry. Taxon, 35: 526-530.
Sanchez-Jimenez I., Pellicer J., Hidalgo O., Garcia S., Garnatje T. and Valles J. (2009). Chromosome Numbers in Three Asteraceae Tribes from Inner Mongolia (China), with Genome Size Data for Cardueae. Folia Geobotanica 44: 307-322.
Stebbins G.L. (1971) Chromosomal evolution in higher plants. London: Edward Arnold Press.
Tabur S., Civelek Ş., Öney S., Yilmaz Ergün Ş.B., Kurşat M. and Türkoğlu I. (2012) Chromosome counts and karyomorphology of some species of Artemisia (Asteraceae) from Turkey. Turkish Journal of Botany 36: 235-246.
Tantray Y.R, Jan I., Saleem Wani M., Kumar Singhal V. and Gupta R.C. (2021) Chromosome numbers and meiotic behaviour in some species of Asteraceae from high altitudinal regions of Kashmir Himalayas. Journal of Asia-Pacific Biodiversity 14(4): 590-606.
Watanabe W. (2002) Index to chromosome numbers in Asteraceae. Available at http://www.asteraceae.cla.kobeu.ac.jp/index.html
Watanabe K., Yahara T., Hashimoto G., Nagatani Y., Soejima A., Kawahara T. and Nakazawa M. (2007). Chromosome Numbers and Karyotypes in Asteraceae. Annals of the Missouri Botanical Garden 94(3): 643-654
Vallès J., Garnatje T., Garcia S., Sanz M. and Korobkov A.A. (2005) Chromosome numbers in the tribes Anthemideae and Inuleae (Asteraceae). Botanical Journal of the Linnean Society 148: 77- 85.
Vallès J. and McArthur E.D. (2001) Artemisia systematics and phylogeny: cytogenetic and molecular insights. In E.D. McArthur and D.J. Fairbanks (Eds.) Artemisia L.: Understanding its evolution 219 Shrub land Ecosystem Genetics and Biodiversity (p.67). Utah: Department of Agriculture Forest Service Press.
Vallès J. and Šiljak-Yakovlev S. (1997) Cytogenetic studies in the genus Artemisia L. (Asteraceae): fluorochrome-banded karyotypes of five taxa, including the Iberian endemic species Artemisia barrelieri Besser. Canadian Journal of Botany 75: 595-606.
Yousofzadeh K., Houshmand S. and Zeinali H. (2010) Karyological study of four Anthemis species from Iran. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 18(1): 55-62.
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