Ferdowsi University of Mashhad

Document Type : Research Articles


1 Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad

2 Department of Medicine, Division of Endocrinology, Brigham & Women's Hospital, Harvard Medical School, Boston 02115, MA, USA

3 Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran



Colorectal cancer is one of the most common cancers and is one of the leading causes of cancer-related deaths worldwide. The underlying biological mechanisms for the development of colorectal cancer are largely unidentified. Several genes have been identified that are most likely involved in the pathogenesis of colorectal cancer disease. However, some other genes might have less evident functions. One gene family with prominent functional roles in the normal colon is mucin. Multiple studies have demonstrated the involvement of mucins in the pathogenesis of human malignancies. Therefore, due to the lack of an inclusive investigation for mucins' expression, mechanism of action, and their involvement in the underlying biology, diagnosis, and prognosis of colon adenocarcinoma, we sought to unearth their potential involvement and their related regulatory networks in this disease. In this investigation, through a stepwise manner, a plethora of databases and algorithmic tools were applied. Due to a significant upregulation at both mRNA and protein levels and following a thorough evaluation of diagnostic and prognostic values in colon adenocarcinoma, MUC13 was determined to be the most relevant regulatory mucin in colon carcinoma. Altogether, these findings indicate a putative ncRNA-mRNA network, including hsa-mir-136-5p, hsa-mir-27a-3p, NEAT1, and XIST to be involved in regulating MUC13 in colon cancer. This stepwise investigation implies that MUC13 may have a crucial role in the underlying molecular mechanisms for the initiation or progression of colon cancer. In addition, it provides insights into molecular mechanisms and possible regulatory non-coding RNA networks that might be responsible for regulating MUC13 expression.