Ferdowsi University of Mashhad

Document Type : Letters / Commentaries

Author

Jahrom University

Abstract

Yellow corky vein is a prevalent disease among navel oranges in the Fars province of Iran. Previously we showed
that a variant of Hop stunt viroid (HSVd-sycv) was associated with the disease. It was closely related to citrus variant
of HSVd from Japan (HSVd-cit8) and with 93.7% homology with lime yellow corky vein variant of HSVd (HSVd–
lycv). In this study, putative RFLP (Restriction Fragment Length Polymorphism) analysis using Vector NTI program
(version 9.0.0) showed that some restriction enzymes could cut HSVd-sycv but not HSVd-lycv. On the other hand,
M.Ngo BIX and FauI which could cut HSVd-lycv at positions of nt 53 and 97, respectively, could not cut HSVdsycv.
Likewise, some restriction enzymes could cut HSVd-cit8 but could not cut HSVd-sycv. Therefore these variants
can be recognized by these restriction enzymes.

Keywords

Amari K., Canizares M. C., Myrta A.,
Sabanadzovic S., Srhiri M., Gavriel I.,
Caglayan K., Varveri C., Gatt M. and Di
Terlizzi B. (2000) First report on Hop stunt
viroid (HSVd) from some Mediterranean
countries. Phytopathologia Mediterranea
39:271-276.
2. Anirban Roy and Ramachandran P. (2003)
Occurrence of a Hop stunt viroid (HSVd)
variant in yellow corky vein disease of citrus
in India. Current Science 85:1608-1612.
3. Avina-Padilla K., de la Vega O. M., Rivera-
Bustamante R., Martinez-Soriano J. P.,
Owens R. A., Hammond R. W. and Vielle-
Calzada J.-P. (2015) In silico prediction and
validation of potential gene targets for
pospiviroid-derived small RNAs during
tomato infection. Gene 564:197-205.
4. Azad P. 1993. Reduction in yield of acid
limes infected with citrus corky vein virus in
Assam. In Proceedings of the 12th
Conference of International Organization
Citrus Virologist. IOCV, Riverside. 458.
5. Bagherian S. and Izadpanah K. 2010. Two
novel variants of hop stunt viroid associated
with yellow corky vein disease of sweet
orange and split bark disorder of sweet lime.
Pp: 105-113. In 21 st International
Conference on Citrus Virus and other Graft
Transmissible Diseases of Fruits
Crops.[Links].
6. Gorsane F., Elleuch A., Hamdi I., Salhi-
Hannachi A. and Fakhfakh H. (2010)
Molecular detection and characterization of
Hop stunt viroid sequence variants from
naturally infected pomegranate (Punica
granatum L.) in Tunisia. Phytopathologia
Mediterranea 49:152-162.
7. Jamshidi E., Jafarpour B., Rouhani H. and
Salehi M. (2014) Association of members of
clover proliferation (16SrVI) and pigeon pea
witches' broom (16SrIX) phytoplasma
groups with tomato big bud disease in Iran.
Iranian Journal of Plant Pathology 50:77-89,
Pe175.
8. Katsarou K., Rao A., Tsagris M. and
Kalantidis K. (2015) Infectious long noncoding
RNAs. Biochimie 117:37-47.
9. Roy A. and Ramachandran P. (2006)
Characterization of a Citrus exocortis viroid
variant in yellow corky vein disease of citrus
in India. Current Science (00113891) 91.
10. Su X., Fu S., Qian Y., Xu Y. and Zhou X.
(2015) Identification of Hop stunt viroid
infecting Citrus limon in China using small
RNAs deep sequencing approach. Virology
journal 12:1.
CAPTCHA Image