Masoumeh Aalipour; Asghar Aalipour; Mohammad Mousaei Ghasroldasht
Abstract
Colorectal cancer (CRC) and gastric cancer (GC) are multifactorial diseases likely influenced by genetic susceptibility. Gastric cancer is also the fourth most common cancer in the world and the second leading cause of cancer-related mortality. CD86 (B7-2) is a costimulatory molecule found on antigen-presenting ...
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Colorectal cancer (CRC) and gastric cancer (GC) are multifactorial diseases likely influenced by genetic susceptibility. Gastric cancer is also the fourth most common cancer in the world and the second leading cause of cancer-related mortality. CD86 (B7-2) is a costimulatory molecule found on antigen-presenting cells (APCs) that is important in autoimmune, transplantation, and tumor immunity. This protein is expressed in the immune system cells and is involved in the pathogenesis of various inflammatory disorders and inflammation. Rs17281995 polymorphism is located in section 3’ UTR, and given the regulatory role of 3' UTR gene sequences, SNPs located in these regions can affect the expression and function of the corresponding protein. In the present study, the relationship between rs17281995 polymorphism located in the 3' UTR regulatory region of the CD86 gene sequence and the risk of colorectal and gastric cancer in Iranian patients was analyzed. Polymorphism was identified in 26 patients with colorectal cancer, 30 patients with gastric cancer, and 36 healthy controls using the high-resolution DNA melting curve analysis (HRM) technique. The Data was then analyzed using SPSS software. There was no significant relationship between rs17281995 polymorphism and colorectal (P = 0.75) and gastric cancers (P = 0.97) in the Iranian population. In addition, genotypic distribution analysis showed no significant difference between the patient and control groups (P˃0.05). Among people with colorectal cancer, 0.577 had the G allele and 0.423 had the C allele. In the control group, 0.639 had the G allele and 0.361 had the C allele. In conclusion, our data indicate that the CD86 rs17281995 gene polymorphism does not seem to be a risk factor for colorectal and gastric cancers in the Iranian population.
Mohammad Reza Izadpanah; Leila Asadpour
Abstract
Staphylococcus aureus is a common pathogen potentially able to cause a wide range of infectious diseases in human and animals and coagulase enzyme is one of the important virulence factors of this bacterium. Polymorphism of the coagulase encoding gene (coa) is one of the molecular-based typing methods ...
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Staphylococcus aureus is a common pathogen potentially able to cause a wide range of infectious diseases in human and animals and coagulase enzyme is one of the important virulence factors of this bacterium. Polymorphism of the coagulase encoding gene (coa) is one of the molecular-based typing methods of S. aureus isolates. In this study, the polymorphism of the coagulase gene among MRSA and MSSA isolates were investigated using PCR-RFLP analysis. To perform coagulase gene typing, the repeated units encoding hypervariable regions of coagulase gene of 30 clinical isolates of S. aureus were amplified by the PCR technique; this was followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP) patterns. In total two amplicons (680 bp and 750 bp) and four distinct RFLP banding patterns (280+400, 340+340, 280+470, and no digested amplicon of 750 bp) were observed. Genotype with PCR-RFLP patterns of 280+400 bp was predominated. The results indicated polymorphism in the investigated regions of coagulase gene. This polymorphism can be used for identification of S. aureus isolates and showing the epidemiological relationship among them.
Sina Gerayli; Alireza Pasdar; Sina Rostami; Samaneh Sepahi; Seyed Mousalreza Hoseini; Reza Jahanian; Aida Gholoobi; Zahra Meshkat; Mitra Ahadi
Abstract
Single nucleotide polymorphism in codon 72 of p53 gene (Arg/Pro) changes p53 protein structure and affects its activities. Hepatitis C virus (HCV) is believed to induce hepatocellular carcinoma and P53 polymorphisms have been associated with human cancers. The aim of this study was to evaluate genetic ...
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Single nucleotide polymorphism in codon 72 of p53 gene (Arg/Pro) changes p53 protein structure and affects its activities. Hepatitis C virus (HCV) is believed to induce hepatocellular carcinoma and P53 polymorphisms have been associated with human cancers. The aim of this study was to evaluate genetic variants of codon 72 of p53 gene polymorphism in HCV patients and its relationship with HCV infection.
The study was conducted on 67 HCV patients, who were referred to medical centers of Mashhad city, Iran, and 73 healthy people from the same region. Genotyping of codon 72 of p53 gene was performed by PCR-RFLP method.
The distributions of different alleles of p53 polymorphisms did not differ significantly between groups. The respective proportions of Proline homozygotes, heterozygotes, and Arginine homozygotes were 37.31%, 35.82%, 26.86% in patients and 39.72%, 27.39%, and 32.87% in the control group respectively. However, we found no significant differenece for the allelic or genotype distribution between cases and controls.
Our results indicated no strong evidence of association of the p53 polymorphism with HCV infection; however, further investigation is needed in different ethnic groups to elucidate the role of this polymorphism in HCV infection.
Zeinab Hosseinnia; Ali Shabani; Hamed Kolangi Miandare
Abstract
Genetic diversity is one of the three levels of biodiversity. The aim of present study was to compare levels of genetic polymorphism between wild Bream populations using seven microsatellite loci. Genetic diversity was investigated by studying samples collected from two regions, the coast of Chamkhale ...
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Genetic diversity is one of the three levels of biodiversity. The aim of present study was to compare levels of genetic polymorphism between wild Bream populations using seven microsatellite loci. Genetic diversity was investigated by studying samples collected from two regions, the coast of Chamkhale and Bandaranzali of Gilan province. A total of seven microsatellite loci (MFW7, MFW26, Mcs1EH, Rser10, Bl1-153, Bl2-114 and IC654) were used. The average number of alleles in Chamkhale and Bandaranzali coast were 10 and 10.71 alleles, respectively. The numbers of effective alleles were 7.05 and 7.74 alleles in each population. Allele frequency was declined in wild fish due to inbreeding and genetic drift. The mean of observed heterozygosity values were 0.66 and 0.70 in Chamkhale and Bandaranzali coast, respectively. Approximately, all of loci showed deviation from Hardy-Weinberg equilibrium. The genetic similarity and distance between the two populations were 0.316 and 0.684, respectively. The results of Molecular Variance Analysis revealed that genetic diversity within locations was 97 percent, while among them was 3 percent. The Fst value was 0.024 that indicates the low genetic differentiation between the two locations which could be explained by the low number of alleles in two populations. Furthermore, the Natural Migration (Nm) between two stations was obtained 16.30. According to the analysis, it seems that Abramis brama has not a desirable genetic diversity in the investigated regions.
Dor Mohammad kordi Tamandani; Azizoallah Mojahed; Maryam Najafi
Abstract
Schizophrenia is a sophisticated mental disability which has affected nearly1.1% of people all over the world. According to recent researches, the key proteins triggered in the immune system are cytokines which might also be taking part in the pathogenesis of schizophrenia. The aim of this study was ...
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Schizophrenia is a sophisticated mental disability which has affected nearly1.1% of people all over the world. According to recent researches, the key proteins triggered in the immune system are cytokines which might also be taking part in the pathogenesis of schizophrenia. The aim of this study was to evaluate the relationship between the -1082G/A and +874T/A polymorphisms of IL-10 and IFN-γ genes, respectively, in patients with schizophrenia. Materials and Methods: Total of 94 schizophrenic patients and 97 individuals as control samples were enrolled in this study. All samples were genotyped by amplification mutation refractory system-polymerase chain reaction (ARMS PCR) for candidate SNPs in IFN-γ and IL-10 genes. Results: No significant association was found among various genotypes of IFN-γ and IL-10 in selected SNPs with risk of schizophrenia, As well as there was no significant variation in allelic frequency of IFN-γ and IL-10 genes with the risk of disease. Conclusion: These data suggest that the -1082G/A of IL-10 and +874T/A IFNγ genes are not involved in the development of schizophrenia risk. To validate of this data, suggesting more studies in diverse populations with larger sample size.
Samaneh Sepahi; Razieh Jalal; Behnaz Toluinia; Ahamad Asoodeh; Jamshid Darvish
Abstract
The prevalence of type 2 diabetes mellitus (T2DM) is rising dramatically in the Middle East, especially in the Islamic Republic of Iran, but the genetic basis of type 2 diabetes in Iran is poorly understood. Polymorphisms of hepatocyte nuclear factor-1α (HNF-1α) and glucagon-like peptide-1 receptor ...
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The prevalence of type 2 diabetes mellitus (T2DM) is rising dramatically in the Middle East, especially in the Islamic Republic of Iran, but the genetic basis of type 2 diabetes in Iran is poorly understood. Polymorphisms of hepatocyte nuclear factor-1α (HNF-1α) and glucagon-like peptide-1 receptor (GLP-1R) genes showed association with type 2 diabetes in several ethnic groups. In this study, we evaluated whether these markers confer susceptibility to T2DM in a diabetic population living in Mashhad (northeast of Iran). Genotyping of Ala98Val (HNF-1α) and Thr149Met (GLP-1R) was done by the restriction fragment length polymorphism-PCR (RFLP-PCR) method in the following groups: 1) early-onset diabetes (age at onset ≤ 35 years); 2) late-onset diabetes (age at onset > 35 years); and 3) control. Our results showed that CT (Ala/Val) genotype of HNF-1α was higher in the early-onset type 2 diabetic group compared to the controls but difference was not significant. We did not find the GLP-1R Thr149Met mutation in all participants. The prevalence of the HNF-1α (Ala98Val) and (GLP-1R) Thr149Met mutations has not been previously reported in Iranian participants. We conclude that these mutations are not a common cause of T2DM in our studied population.
Moslem Shojaei; Mohammadreza Mohammad Abadi; Masood Asadi Fozi; Omid Dayani; Amin Khezri; Masoumeh Akhondi
Abstract
Identification of genes affecting energy balance, milk yield and feed intake is an interesting area of
researches in animal breeding. Leptin gene polymorphism is associated with key economic affair. Considering
rich resources for animals, in our country, accomplishing a few assays to identify a gene ...
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Identification of genes affecting energy balance, milk yield and feed intake is an interesting area of
researches in animal breeding. Leptin gene polymorphism is associated with key economic affair. Considering
rich resources for animals, in our country, accomplishing a few assays to identify a gene that controls her traits
with molecular genetics, and identifying the candidate genes in sheep breeds using DNA test can greatly help
to her breeding progress. For analyzing Leptin gene polymorphism and its association with growth traits in
Kermani sheep, blood samples of 120 sheep of both genes rearing at breeding centre of Shahre Babak were
taken. In addition growth traits were measured. PCR was performed to amplify 275 bp fragments of exon 3
from Leptin gene. Then Single Strand Conformation Polymorphism (SSCP) of PCR product was performed
and Leptin band patterns (genotypes) were obtained using acrylamid gel and silver staining. For Leptin gene 10
genotypes including A/A, C/C, A/B, A/C, A/B/C, A/B/E, A/B/F, A/C/F, A/B/D/E and A/B/C/F were obtained.
The results of this study showed that the growth traits are significantly affected by the genotypes. Accordingly,
A/B/E, A/C, A/B/C/F and A/B/C/F genotypes had higher body weight at 3, 6, 9 and 12 months of ages
respectively. The animals with A/B, A/B, A/B/F and A/B/D/E genotypes had the smallest body weight at 3, 6,
9 and 12 months of ages respectively. It is suggested that polymorphism in Leptin gene loci can be used as a
selective criterion to improve growth traits in Kermani sheep.
