Sina Gerayli; Alireza Pasdar; Sina Rostami; Samaneh Sepahi; Seyed Mousalreza Hoseini; Reza Jahanian; Aida Gholoobi; Zahra Meshkat; Mitra Ahadi
Abstract
Single nucleotide polymorphism in codon 72 of p53 gene (Arg/Pro) changes p53 protein structure and affects its activities. Hepatitis C virus (HCV) is believed to induce hepatocellular carcinoma and P53 polymorphisms have been associated with human cancers. The aim of this study was to evaluate genetic ...
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Single nucleotide polymorphism in codon 72 of p53 gene (Arg/Pro) changes p53 protein structure and affects its activities. Hepatitis C virus (HCV) is believed to induce hepatocellular carcinoma and P53 polymorphisms have been associated with human cancers. The aim of this study was to evaluate genetic variants of codon 72 of p53 gene polymorphism in HCV patients and its relationship with HCV infection.
The study was conducted on 67 HCV patients, who were referred to medical centers of Mashhad city, Iran, and 73 healthy people from the same region. Genotyping of codon 72 of p53 gene was performed by PCR-RFLP method.
The distributions of different alleles of p53 polymorphisms did not differ significantly between groups. The respective proportions of Proline homozygotes, heterozygotes, and Arginine homozygotes were 37.31%, 35.82%, 26.86% in patients and 39.72%, 27.39%, and 32.87% in the control group respectively. However, we found no significant differenece for the allelic or genotype distribution between cases and controls.
Our results indicated no strong evidence of association of the p53 polymorphism with HCV infection; however, further investigation is needed in different ethnic groups to elucidate the role of this polymorphism in HCV infection.
Farahnaz Molavi; Jamshid Darvish; Farhang Haddad; Maryam M. Matin
Abstract
Cytotaxonomy is a branch of cytogenetics, devoted to the comparative study of karyological features for systematic and evolutionary purposes. Surely, awareness of chromosomal characters increases our knowledge in different fields of studies. In this study, cytogenetic analyses were performed in 92 Mus ...
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Cytotaxonomy is a branch of cytogenetics, devoted to the comparative study of karyological features for systematic and evolutionary purposes. Surely, awareness of chromosomal characters increases our knowledge in different fields of studies. In this study, cytogenetic analyses were performed in 92 Mus musculus specimens from 26 localities in Iran. Cytogenetic characteristics of the house mouse, Mus musculus, in Iran show that the chromosome number is 2n=40 and the arm number is NF=40. The karyotyping results indicated the presence of 20 Acrocentric (A) chromosome pairs. The L/S (r ratio) was between 2.0621 and 4.5862. The length of shortest chromosome, length of longest chromosome and mean of chromosomal length in different populations were between 2-3.58, 6.07-7.01 and 3.43-5.05 (μm), respectively. The results showed two distinct karyotypic formulae, namely cytotype B and cytotype C. Asymmetry indexes (AI, DI, As%, A, A2, A1 and Syi%) in all population except Birjand and Khash showed symmetry in chromosomes. In clustering methods using the matrix of symmetrical indexes similarities, four clusters were revealed, one for specimens of central and east of Iran, the second cluster for specimens from south and west of Iran, the third cluster was related to the eight specimens of Birjand and finally, the fourth cluster for two specimens of Khash locality
Fatemeh Nasernakhaei; Mohammad Reza Rahiminejad; Hojjatollah Saeidi; Manoochehr Tavassoli
Abstract
As a case study, the genotypic polymorphism of the Iranian material of diploid Triticum was screened based on internal transcribed spacer 2 (ITS2) of the ribosomal DNA (rDNA) using single strand conformation polymorphism (SSCP) analysis. This is a simple and cost effective technique for genotyping and ...
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As a case study, the genotypic polymorphism of the Iranian material of diploid Triticum was screened based on internal transcribed spacer 2 (ITS2) of the ribosomal DNA (rDNA) using single strand conformation polymorphism (SSCP) analysis. This is a simple and cost effective technique for genotyping and investigating the allelic polymorphism among plant populations. Performing SSCP analysis among 21 accessions, two distinctive migration profiles associated with the two validly recognized diploid Triticum species i.e., T. monococcum subsp. aegilopoides and T. urartu were detected. Nucleotide sequencing confirmed the distinction of two types of observed profiles. This study suggested that SSCP is an applicable diagnostic molecular tool for screening the genotypic variability before the costly direct genomic sequencing is begun.
Faranaz Molavi; Haji GholiKami; Morteza Yazdanpanahi
Abstract
The diploid number among Gekkonid lizards ranges from 2n=16 to 2n=46. The majority of geckos' chromosomes are small which makes the study of their chromosomes very difficult. For this reason, karyotype of Cyrtopodion caspium and some similar gecko are still not known. The close relatives of C. caspium ...
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The diploid number among Gekkonid lizards ranges from 2n=16 to 2n=46. The majority of geckos' chromosomes are small which makes the study of their chromosomes very difficult. For this reason, karyotype of Cyrtopodion caspium and some similar gecko are still not known. The close relatives of C. caspium have variation in the chromosomal number in their populations. So to find out possible variability among these populations in Iran. We described the karyotype of testis and bone marrow of 14 specimens of this species from seven provinces and 10 localities in north and north-eastern parts of Iran in 2011. They showed 2n=38 for all populations. There is not intraspecific chromosome polymorphism in Cyrtopodion caspium. The karyotype consists of one pair of metacentric element and 17 pairs of telocentric or subtelocentric elements and one pair of three arm chromosomes. The number of haploid chromosomes was 21 for three male specimens. The chromatid separation did not show heteromorphic sex chromosomes. Our results highlight the need for continued research into the basic biology and taxonomy of Cyrtopodion caspium in Iran.
Massoud Ranjbar; Somayeh Karami
Abstract
In this study, we examined the chromosome number, detailed male meiosis, microsporogenesis, pollen fertility and distribution of cytotypes of Diplotaxis harra (Forssk.) Boiss. This is the first cytogenetic report of the taxon. The two of the populations scored now from regions of the central showed aneuploid ...
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In this study, we examined the chromosome number, detailed male meiosis, microsporogenesis, pollen fertility and distribution of cytotypes of Diplotaxis harra (Forssk.) Boiss. This is the first cytogenetic report of the taxon. The two of the populations scored now from regions of the central showed aneuploid (x = 14) meiotic chromosome count and one of the populations studied from the existed at the diploid level (x = 13). It seems that the species with x = 14 are derived through aneuploid increase. The most prominent among these meiotic abnormalities was the chromatin stickiness which involved inter PMC and fragmented chromosome at different stages of meiosis. Consequently, these populations exhibited varying percentages of pollen sterility and pollen grains of smaller sizes. The aim of the present research was to study the male meiosis in detail and to find the impact of chromatin stickiness in inducing meiotic aberrations and their consequent effect on pollen fertility and pollen size, also to differentiate the individuals growing wild and also to find out the distribution patterns of different cytotypes in the Iran.
Roya Karamian; Massoud Ranjbar; Anahita Hadadi
Abstract
In this study the original mitotic chromosome counts are presented for 5 Onobrychis species of O. sect. Onobrychis in Iran, 2n = 2x = 14 for O. persica, 2n = 4x = 28 for O. viciifolia, 2n = 4x = 28 for O. altissima, 2n = 2x = 14 for O. shahpurensis and 2n = 2x = 14 for O. sosnovskyi. The basic chromosome ...
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In this study the original mitotic chromosome counts are presented for 5 Onobrychis species of O. sect. Onobrychis in Iran, 2n = 2x = 14 for O. persica, 2n = 4x = 28 for O. viciifolia, 2n = 4x = 28 for O. altissima, 2n = 2x = 14 for O. shahpurensis and 2n = 2x = 14 for O. sosnovskyi. The basic chromosome numbers of all studied taxa are consistent with the proposed base number of x = 7. In addition, the meiotic chromosome number of 2n = 4x = 28 for O. viciifolia and O. altissima and of 2n = 2x = 14 for O. sosnovskyi and O. persica are reported here. This study is the first report on the chromosome counts of O. persica and O. shahpurensis. All studied taxa displayed regular bivalent pairing and chromosome segregation at meiosis. However, some abnormalities were observed in the taxa are discussed.
Masood Ranjbar; Zahra Hajmoradi; Roya Karamian
Abstract
In the present paper, the cytogenetic study including meiotic chromosome number and behavior along with
pollen viability were performed in 4 populations of Trigonella spruneriana Boiss. This is the first cytogenetic
report of the taxon. All populations are diploid and possess 2n = 2x = 16 chromosome ...
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In the present paper, the cytogenetic study including meiotic chromosome number and behavior along with
pollen viability were performed in 4 populations of Trigonella spruneriana Boiss. This is the first cytogenetic
report of the taxon. All populations are diploid and possess 2n = 2x = 16 chromosome number, which is
consistent with the proposed basic number of x = 8. In addition, some meiotic irregularities observed in
different populations included chromosomes stickiness, B-chromosomes, chromosome bridges resulting from
stickiness, the occurrence of laggard chromosomes, formation of micronuclei in tetrad cells and cytomixis. The
highest and the lowest percentages of pollen viability were observed in populations SPR 658 and SPR 566,
respectively.
