Soraya Salarizadeh; Hamid Reza Kavousi
Abstract
Cadmium, a metal widely used in industrial processes, has been recognized to be a highly toxic and dangerous environmental pollutant. Random amplified polymorphic DNA (RAPD) test is a feasible method to evaluate the toxicity of environmental pollutants on vegetal organisms. Herein, two Iranian ecotypes ...
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Cadmium, a metal widely used in industrial processes, has been recognized to be a highly toxic and dangerous environmental pollutant. Random amplified polymorphic DNA (RAPD) test is a feasible method to evaluate the toxicity of environmental pollutants on vegetal organisms. Herein, two Iranian ecotypes of Cuminum cyminum (cumin) plantlets following exposure to cadmium (Cd) concentrations of 300–1050 µM for 7 days were screened for DNA genetic alterations by DNA fingerprinting. 10 RAPD primers of 50–70% GC content were found to produce unique polymorphic band profiles on treating cumin seedlings. After Cd treatment, significant changes were observed in RAPD profiles of both ecotypes. These changes included variation in band intensity, disappearance of bands, and appearance of new PCR products in comparison to the control group, and they were dose dependent. These results indicated that genomic template stability (GTS, a qualitative measure reflecting changes in RAPD profiles) was significantly affected at the above Cd concentrations. The GTS index in both ecotypes gradually decreased with an increase in Cd concentrations. These findings suggest that DNA polymorphism detected by RAPD analysis could be a powerful eco-toxicological tool to evaluate the genotoxic effects of cadmium on plants.
Maryam Peyvandi; Mahnaz Monsef; Mehdi Hosseini Mazinani
Abstract
The effect of sub culturing frequencies and different cytokinins (benzyl amino purine (BAP), 2-isopentenyl adenine (2ip) on genetic stability of micropropagated shoots from olive plant were investigated by using physiological traits and Random Amplified Polymorphic DNA (RAPD) markers. Axillary buds of ...
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The effect of sub culturing frequencies and different cytokinins (benzyl amino purine (BAP), 2-isopentenyl adenine (2ip) on genetic stability of micropropagated shoots from olive plant were investigated by using physiological traits and Random Amplified Polymorphic DNA (RAPD) markers. Axillary buds of the olive (cv. Dezful) plants were cultured and subcultured in DKW medium, supplemented with BAP (4 mgl-1) or 2-ip (4 mgl-1). In different hormone media and subcultures there were not significant differences in shoot proliferation rate. To amplify DNA, 18 arbitrary decamer primers were screened, out of which 16 primers generated clear and reproducible bands. All RAPD profiles from the micropropagated plants were monomorphic. The treatments included four successive subcultures in two hormone treatments (2ip or BAP) (4 mgl-1) The 16 primers produced a total of 213 (an average of 13.31 band per primer) scorable bands. The dendrogram constructed on the basis of jaccard’s similarity matrix, followed by UPGMA based clustering analysis showed that micropropagated plants were genetically stable and similar to the mother plant.
