Zahra Sepehry Javan
Abstract
Salvia is the largest genus in the Lamiaceae family in the world and Iran contains 58 species. This genus contains over 900 species of annual and perennial herbaceous plants and differs from other lamias in the unusual structure of their plumage. Information on important medicinal plants' genetic diversity ...
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Salvia is the largest genus in the Lamiaceae family in the world and Iran contains 58 species. This genus contains over 900 species of annual and perennial herbaceous plants and differs from other lamias in the unusual structure of their plumage. Information on important medicinal plants' genetic diversity and population structure is well documented in the literature. Increased genetic diversity can reduce the negative effects of inbreeding on populations. We investigated genetic variation among eight Salvia species using ISSR molecular markers. ISSR is a molecular marker amplified by PCR using microsatellite primers. Samples were gathered from various locations in Iran. Most samples were assigned to Ardabil city. Twenty randomized ISSR primers were used, generating different polymorphic bands. The 20 ISSR primers generated 225 valuable bands and 221 bands were polymorphic (98/2%). Pairwise genetic distances ranged from 0.083 to 0.577. Dendrograms were generated using the UPGMA method using NTSYSpc 2.02i software to identify seven major groups from eight Salvia species. The polymorphism levels observed in the present study represent a high degree of genetic diversity among Salvia species. Following the first study on the genetic relationships of eight species of Salvia in Iran using RAPD and ISSR molecular markers, this study was performed using the ISSR molecular marker to investigate the genetic relationships of eight other species of Salvia. The results of this research represented that the molecular markers of the ISSR are suitable for assessing genetic variation and evolutionary relationships among Salvia species, showing a wide range of dispersal.
Behnaz Safamanesh; Sedigheh Esmaeilzadeh Bahabadi; Ali Izanloo
Abstract
Barberry fruit is a medicinal plant, and it is one of the most important horticultural crops in South Khorasan province, Iran. Genetic diversity has a basic role in the successful breeding of crop varieties with durable resistance to biotic and abiotic stresses. The main objective of this study was to ...
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Barberry fruit is a medicinal plant, and it is one of the most important horticultural crops in South Khorasan province, Iran. Genetic diversity has a basic role in the successful breeding of crop varieties with durable resistance to biotic and abiotic stresses. The main objective of this study was to assess the genetic diversity of 20 ecotypes of Berberis, which collected from different regions of South Khorasan province, Iran, using ISSR and SSR markers. In this study, 10 ISSR primers and 5 SSR primer pairs, with the amplification of suitable polymorphic alleles were used. A total of 98 bands for ISSR markers and 43 bands for SSR markers were detected between 300 to 1300 bp and 100 to 1100 bp in size, respectively. Polymorphic ISSR-7 and CA03 primers amplified the highest number of alleles with 17 and 24 bands, respectively, while ISCS50 and CA30 primers amplified only two polymorphic alleles. The ISCS57 and GA31 primers had the highest polymorphic information content (PIC) and ISCS50 and GA04 primers had the lowest PIC. The estimated Nei's and Shannon indices for genetic diversity in ISSR markers were 0.24 and 0.35, while for SSR markers these were 0.23 and 0.34, respectively. Based on cluster analysis, five and six main groups were identified for ISSR and SSR markers, showing high genetic variations among a set of collected barberry ecotypes. Analysis of molecular variances in both ISSR and SSR markers showed that high level of total variation was due to within populations, rather. Therefore, it will be better to select within populations in breeding programs.
