Mahnaz Kiani; Homa Zarghami; Farshid Memariani; Ali Tehranifar
Abstract
Tissue culture methods provide tools to supplement traditional methods for collection, propagation and
preservation of endangered plant species. In this study, in vitro propagation of Diaphanoptera khorasanica
Rech.f., a rare and threatened plant species with limited distribution range and population ...
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Tissue culture methods provide tools to supplement traditional methods for collection, propagation and
preservation of endangered plant species. In this study, in vitro propagation of Diaphanoptera khorasanica
Rech.f., a rare and threatened plant species with limited distribution range and population was investigated.
This species has a potential as an ornamental plant. Single node explants were provided from both adult and
seedling sources. Several disinfection treatments were tried to permit selection of a suitable method. Different
growth regulators were used for establishment, proliferation and rooting stages. Explants showed the highest
establishment percentage after 5 min treatment with 1% sodium hypochlorite (NaOCl), cultured in MS medium
containing 2.2 μM 6-benzylaminopurine (BAP) and 2.4 μM indole-3-butyric acid (IBA). The highest
proliferation of explants from both adult and seedling source explants was obtained from media supplemented
by BA treatment in contrast to TDZ. No significant differences were found between different concentrations of
BAP and TDZ. Proliferated shoots in TDZ were longer and had more internode length and less vitrification, in
comparison with those in BAP. In vitro rooting of proliferated shoots just induced in liquid half-strength MS
medium and rooting was not observed in solid medium. The shoots that originated from adult plants gave rise
to the highest rooting rate with 4.8μM α-naphthalene acetic acid (NAA) and 2.4 μM, but NAA rooted plantlets
showed higher survival percentage in acclimatization step. This study was aimed towards developing an
efficient protocol for in vitro propagation of D. khorasanica and conservation of this vulnerable species.
