Roya Karamian; Massoud Ranjbar; Anahita Hadadi
Abstract
In this study the original mitotic chromosome counts are presented for 5 Onobrychis species of O. sect. Onobrychis in Iran, 2n = 2x = 14 for O. persica, 2n = 4x = 28 for O. viciifolia, 2n = 4x = 28 for O. altissima, 2n = 2x = 14 for O. shahpurensis and 2n = 2x = 14 for O. sosnovskyi. The basic chromosome ...
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In this study the original mitotic chromosome counts are presented for 5 Onobrychis species of O. sect. Onobrychis in Iran, 2n = 2x = 14 for O. persica, 2n = 4x = 28 for O. viciifolia, 2n = 4x = 28 for O. altissima, 2n = 2x = 14 for O. shahpurensis and 2n = 2x = 14 for O. sosnovskyi. The basic chromosome numbers of all studied taxa are consistent with the proposed base number of x = 7. In addition, the meiotic chromosome number of 2n = 4x = 28 for O. viciifolia and O. altissima and of 2n = 2x = 14 for O. sosnovskyi and O. persica are reported here. This study is the first report on the chromosome counts of O. persica and O. shahpurensis. All studied taxa displayed regular bivalent pairing and chromosome segregation at meiosis. However, some abnormalities were observed in the taxa are discussed.
Mahnaz Kiani; Homa Zarghami; Ali Tehranifar; Farshid Memariani
Abstract
In vitro methods provide a variety of tools to supplement traditional methods for collection, propagation and
preservation of endangered plant species. In this study, an efficient protocol was developed for in vitro
propagation of Colutea gifana, a rare and endangered plant species with limited reproductive ...
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In vitro methods provide a variety of tools to supplement traditional methods for collection, propagation and
preservation of endangered plant species. In this study, an efficient protocol was developed for in vitro
propagation of Colutea gifana, a rare and endangered plant species with limited reproductive capacity that
grows in a narrow area of Iran. Single node explants were used for a series of experiments to select the
appropriate disinfection method and growth regulators for establishment, proliferation and rooting stages.
Explants showed the highest establishment percent after treatment with 2% Sodium hypochlorite (NaOCl) for
15 min, cultured in MS medium containing 2.2 μM 6-benzylaminopurine (BA) and 1 μM indole-3-butyric acid
(IBA). In proliferation stage, 8.8 μM of BA was more effective cytokinin than Kinetin (Kin) and Thidiazuron
(TDZ) for growth induction of axillary shoots. In vitro rooting of proliferated shoots was induced in halfstrength
MS medium in all concentrations of both tested auxins i.e. IBA and α-naphthalene acetic acid (NAA).
Eighty percent of the plantlets were successfully acclimatized to ex vitro conditions, showed normal
development. These plants were used to replenish declining populations in the collection sites and conserve C.
gifana from extinction.
Masoud Ranjbar; Roya Karamian; Fatemeh Hajmoradi
Abstract
The genus Onobrychis belongs to family Fabaceae and has about 130 species throughout the world.
Onobrychis sect. Hymenobrychis with nearly 14 species in Iran is one of the important sections of the genus.
The chromosome number and meiotic behaviour were studied in two populations of Onobrychis chorassanica
belonging ...
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The genus Onobrychis belongs to family Fabaceae and has about 130 species throughout the world.
Onobrychis sect. Hymenobrychis with nearly 14 species in Iran is one of the important sections of the genus.
The chromosome number and meiotic behaviour were studied in two populations of Onobrychis chorassanica
belonging to this section native to Iran. This report is the first cytogenetic analysis of this taxon. Both
populations are diploid and possess 2n = 2x = 14 chromosome number, consistent with the proposed base
number of x = 7. Although these taxa displayed regular bivalent pairing and chromosome segregation at
meiosis, some meiotic abnormalities were observed. The meiotic abnormalities included varied degrees of
chromosome stickiness including B-chromosome, cytomixis, asynchronous nucleus, fragmented chromosomes,
occurrence of laggard chromosomes, chromosome bridges, desynapsis, micronucleous and tripolar cells.
