Morteza Hashemi Attar; Mohammad Reza Nassiri
Abstract
Preserving genetic diversity of Iranian native chickens is significant in order to perform breeding programs and improved production. Genetic diversity among close populations can be determined by investigating their phylogenetic relations. Different approaches have been applied to determine the phylogenic ...
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Preserving genetic diversity of Iranian native chickens is significant in order to perform breeding programs and improved production. Genetic diversity among close populations can be determined by investigating their phylogenetic relations. Different approaches have been applied to determine the phylogenic relations from which genomic sequencing has been considered the most functional approach. In this project, we aimed to evaluate the phylogeny and genetic nucleotide sequences of ND4, ND4L regions in mitochondrial genome of Khorasan native chickens. Blood samples were collected from randomly 6 Khorasan native chicken populations and DNA were extracted. ND4L, ND4 regions were amplified using specific primers of 913 and 802 bp length, respectively. Sequencing was don according to Sanger method based on automated system. In comparison with other breeds (data extracted from NCBI), phylogeny trees and matrices regarding their genetic diversity with Khorasan native chickens for ND4, ND4L were drawn. Results showed that there is no haplotype difference between the studied samples. Lowest genetic distance was observed between Khorasan native chicken and other Asian chickens i.e. Jiangbian, Lvenwv and Red jungle fowl for the ND4, ND4L genes indicating their close relationship.
Javad Karimi; Mahnaz Hassani-Kakhki; Mehdi Modarres Awal
Abstract
Thrips tabaci Lindeman is an extensively distributed pest insect in many areas that affects plants through
direct feeding and at the same time, it makes damage as a vector of different viruses. As a basic first step to
control pests is authentic identification, but the inability to determine morphological ...
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Thrips tabaci Lindeman is an extensively distributed pest insect in many areas that affects plants through
direct feeding and at the same time, it makes damage as a vector of different viruses. As a basic first step to
control pests is authentic identification, but the inability to determine morphological characters of thrips
species makes this process very difficult. For creating an identification key for T. tabaci, an economically
important species present in Iran, four individuals were selected from four different sites of Mashhad and the
vicinity, each as a separate population. The method was based on nucleotide sequencing analysis of the
mitochondrial cytochrome C oxidase I (COI) gene. Phylogenetic analyses conducted by the neighbor-joining
method yielded almost identical phylogenetic reconstructions of trees that separated thrips based on the
geographic origin. Molecular data indicate that different thrips species are located in distinct groups. These
results show that molecular keys can be a useful method to provide much-needed information on thrips
identification for pest management officers and quarantine purposes.
