Zahra Jomehzadeh; Farhang Haddad; Maryam M. Matin; Shokouhozaman Soleymanifard
Abstract
There are several studies suggesting the role of aneuploidy in tumor formation. Aneuploid cells are different from normal ones in term of gene expression and proteome. Cells with different amount and kind of proteins might act differently to external stimuli, including ionizing irradiation. ...
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There are several studies suggesting the role of aneuploidy in tumor formation. Aneuploid cells are different from normal ones in term of gene expression and proteome. Cells with different amount and kind of proteins might act differently to external stimuli, including ionizing irradiation. Currently, radiotherapy is one of the main methods in fight against cancer, therefore, it is important to understand the response of the aneuploidy-tumor cells to irradiation. To investigate the chromosomal effect of gamma irradiation on aneuploid cells, L929 cells were treated with 1.5 ng.ml-1 of vinblastine to induce aneuploidy. Vinblastine-treated cells were left to recover for 72 h and irradiated with 1 Gy of gamma radiation. Induced chromosomal damages were investigated using micronucleus (Mn) assay. Data showed that vinblastine and gamma irradiation both were able to significantly increase micronucleated-binucleated cells (MnBi) frequency. However, 1 Gy gamma irradiation of the cells after 72 h of vinblastine treatment led to the lower frequency of MnBi compared to irradiated cells. Results of this study suggest that vinblastine treatment of cells before irradiation not only did not sensitize the cells to radiation-induced chromosomal abnormalities, but also had radio-protective effect for these cells. This result could be useful in planning cancer therapy regimes.
Farhang Haddad; Vajiheh Golami; Maliheh Pirayesh Shirazi-Nejad
Abstract
Due to the wide range of applications for ozone and its increasing use for medical and industrial purposes, studying its effects has become a very important line of research. The ozone has been suspected to be a carsinogen. Because of the increasing use of ozone, the human could be more and more exposed ...
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Due to the wide range of applications for ozone and its increasing use for medical and industrial purposes, studying its effects has become a very important line of research. The ozone has been suspected to be a carsinogen. Because of the increasing use of ozone, the human could be more and more exposed to this gas. In this study the effects of ozone inhalation on chromosomes and its clastogenic consequences have been investigated using in vivo micronucleus assay in bone marrow cells of treated rats.
Animals were treated for 6 hours a day at 3 ppm of ozone during 10 consecutive days. The micronucleus assay was performed immediately and 11 days after the last exposure. The frequency of micronucleated polychromatic erythrocyte of bone marrow (MNPCE) increased in both groups compared to the control. Such increase confirmed the clastogenic effects of ozone. The elevated frequency of MNPCE did not decrease after 11 days of the last ozone exposure.
Results indicate that ozone inhalation could induce persistent chromosomal damages even to bone marrow cells which were not in direct contact to it. Also, once more, the results confirmed the usefulness of the micronucleus assay in toxicological studies.
