Maryam Yazdani; Ali Bidmeshkipour; Sajjad Sisakhtnezhad
Abstract
The immunomodulation ability of mesenchymal stem cells (MSCs) has attracted interest as a unique property that makes them interesting tools for the treatment of inflammatory and autoimmune diseases. Eugenol is a volatile compound from the phenylpropanoids class of chemical compounds. Despite extensive ...
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The immunomodulation ability of mesenchymal stem cells (MSCs) has attracted interest as a unique property that makes them interesting tools for the treatment of inflammatory and autoimmune diseases. Eugenol is a volatile compound from the phenylpropanoids class of chemical compounds. Despite extensive investigations on the biological and pharmacological properties of Eugenol, its effect on the MSCs characteristics remains to be clarified. Therefore, this study was designed to evaluate the effect of Eugenol on the expression of genes (Tlr3, Tlr4, Ccl2, and Ccl3) involved in immunomodulation potency of MSCs by quantitative real-time PCR (qRT-PCR). To do so, MSCs were isolated from 4-8 weeks old mouse bone marrow (BM). The effect of Eugenol on the viability of BM-MSCs was evaluated by MTT assay at 24, 48, and 72h after treatment. The results showed that Eugenol reduced the number of BM-MSCs in a dose- and time-dependent manner. In addition, the half maximum inhibitory concentration of Eugenol on MSCs was 400μg/ml at 24 and 48h and 200μg/ml at 72h after treatment. Moreover, about 90% of MSCs were alive at the concentration of 12.5μg/ml 24h after treatment. The qRT-PCR results indicated that Tlr3, Tlr4, Ccl2, and Ccl3 genes up-regulated 1.6-, 1.8-, 1.3-, 2.2-fold, respectively, in Eugenol-treated BM-MSCs compared to untreated controls. In conclusion, we declare that Eugenol may somewhat regulate the immunomodulation potency of MSCs and this study provides a background for further studies on the effect of Eugenol on MSCs characteristics and functions, which may finally improve their potency for cell-based therapy applications.
Mohammad Reza Nikravesh; Mehdi Jalali; Abbas Ali Moeen; Shabnam Mohammadi; Mohammad Hasan Karimfar
Abstract
Nicotine is an alkaloid by high level of addictive property that can quickly assimilate from smoker’s lung. It
passes from the placenta and gathers in the developing fetus. Our previous study showed that collagen type IV
plays a critical role in basement membrane of different embryonic organs. In ...
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Nicotine is an alkaloid by high level of addictive property that can quickly assimilate from smoker’s lung. It
passes from the placenta and gathers in the developing fetus. Our previous study showed that collagen type IV
plays a critical role in basement membrane of different embryonic organs. In this study the effect of maternal
nicotine was evaluated by collagen IV changes in lung of mice offspring during pre and postnatal period.
Pregnant Balb/C mice were divided into 2 experimental and 2 control groups. Experimental group 1 received 3
mg/kg nicotine intrapritoneally from day 5 of gestation to last day of pregnancy. Experimental group 2 received
the same amount of nicotine during the same gestational days as well as 2 first week after birth. The control
groups received the same volume of normal saline during the same periods. At the end of exposure times, all
newborns were anesthetized and their lungs were removed and immunohistochemical study for tracing
collagen was carried out. Our results showed that collagen reaction in the bronchial basement membrane
(BBM) and extra cellular matrix (ECM) of the lung parenchyma experienced a remarkable increase when
compared to the control ones. Cell necrosis definition in lung parenchyma of the experimental group 2 was the
other finding that our investigation revealed. These data indicate that maternal nicotine exposure may induce a
noticeable collagen increase with a reasonable amount in BBM and ECM of respiratory system of next
generation.
Mohammad Reza Nikravesh; M Jalali; MH Karimfar; AA Moeen; Saeedi Nejat Sh; Sh Mohammadi; H Rafighdoust
Abstract
Abstract
Basement membrane of glomerular mesangium (BMG) is a thin membrane which helps to support the capillary loops in a renal glomerulus and type IV collagen is require for complete BM formation during glomerulogenesis. In this investigation specific antibody of type IV collagen has been used in ...
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Abstract
Basement membrane of glomerular mesangium (BMG) is a thin membrane which helps to support the capillary loops in a renal glomerulus and type IV collagen is require for complete BM formation during glomerulogenesis. In this investigation specific antibody of type IV collagen has been used in light microscopy to study development of BMG of the embryonic and postnatal mouse glomerular mesangium. In this study, 20 female Balb/C mice were selected randomly and finding vaginal plug was assumed as day zero of pregnancy. 12 pregnant mice were sacrified by cervical dislocation in one of gestational days 13-18, their fetuses were fixed and serially sectioned. Immunohistochemical Study for tracing of collagen type IV in BMG was carried out. The same processes were carried out for kidneys preparation of pups on 5, 10, 15 and 20 days after birth (2 mothers for each day). The result of the present study revealed that collagen IV reaction was weak on day 15 of gestation. The amount of collagen increased continuously until next days of fetal life and primary of 10 days postnatal in BMG. After this period, collagen IV showed no significant change in newborns. These data indicate that collagen IV appears just during the glomerular vasculogenesis and because of continuity and glomerular endothelial cell differentiation, type IV collagen, is the major structural protein in BMG, have been implicated in these processes.
Keywords: collagen IV, glomerular basement membrane, kidney, mouse
