Kamran Safavi; Gholamreza Kavoosi; Roghayeh Siahbalaei
Abstract
The influence of ZnSO4, FeSO4, ZnO, and Fe2O3 at 80 µM on the expression of desaturase genes and fatty acid composition of Pleurotus ostreatus was investigated. Fatty acid was extracted by lipid extraction and methylation procedure using acidic methanol: normal saline: hexane solution followed by gas ...
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The influence of ZnSO4, FeSO4, ZnO, and Fe2O3 at 80 µM on the expression of desaturase genes and fatty acid composition of Pleurotus ostreatus was investigated. Fatty acid was extracted by lipid extraction and methylation procedure using acidic methanol: normal saline: hexane solution followed by gas chromatography-mass spectrometry (GC-MS). The most prominent fatty acids in P. ostreatus were linoleic acid (44.7%), palmitic acid (8.6%), oleic acid (8.5%), stearic acid (2.9%), pentadecanoic acid (2.6%) and heptadecanoic acid (2.3%). ZnO strongly and Fe2O3 slightly lead a decrease in unsaturated fatty acid (UFA), polyunsaturated fatty acid (PUFA) and omega-6 and an increase in the monounsaturated fatty acid (MUFA), omega-7 and omega-9 content. Accordingly, our results considerably confirmed the different effects of ZnSO4 and ZnO on fatty acid content. These differential effects for FeSO4 and Fe2O3 were not significant. Δ9-desaturase and Δ12-desaturase expression but not Δ15-desaturase were upregulated in the seeds cultured in media containing ZnO and Fe2O3.
Alireza Mirzaei; Sara Dezhsetan; Gholamreza Kavoosi; Mahdi Behnamian
Abstract
Stevia rebaudiana bertoni is a plant with sweetening properties. This medicinal plant is perennial and from Asteraceae family. Its leaves contain glycoside compounds of a sugar part and non-sugar sectors. One of the glycosides compounds is RebaudiosideA which has a greater importance in market. Several ...
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Stevia rebaudiana bertoni is a plant with sweetening properties. This medicinal plant is perennial and from Asteraceae family. Its leaves contain glycoside compounds of a sugar part and non-sugar sectors. One of the glycosides compounds is RebaudiosideA which has a greater importance in market. Several key regulating genes including copalyl diphosphate synthase (CPPS) (AF034545.1), geranylgeranyl diphosphate synthase (GGDPS) (DQ432013.3), (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (HDS) (FJ755689.1), UDP glucosyltranserase 85C2(UGT85C2)(AY345978.1), UDPglucosyltranserase–74G1 (UGT74G1) (AY345982.1) and UDP glucosyltranserase–76G1 (UGT76G1) (KC631816.1) are involved in the biosynthesis Rebaudioside A. This experiment was conducted to evaluate the effect of silver oxide Ag2O on the mRNA level of these genes in the stevia rebaudiana. The experiments repeated 3 times and with concentrations of 50, 100 and 200 µM. Increasing concentrations of 50 micromoles of silver oxide up to 100 micromoles leads to an increase in the expression levels of all the studied genes. Also according to the expression profile of these genes and the results of HPLC there is a significant increase on the expression level of the genes and production of RebaudiosideA under Ag2O treatment. In general, it was found that increasing the concentration of Ag2O can lead to an increase in the level of mRNA for the chosen genes. On the other hand, the low expression of the genes studied under control conditions (No Treatment), compared to the treatment with Ag2O, revealed that the treatment can lead to higher sweetener glycoside components in the Stevia leaves. The physiological assay showed that Ag2O treatment in concentrations of 100 and 200 µM have more positive effect on chlorophyll, protein, carbohydrates and carotenoids in Stevia.
