Ehsan Nasr; Hrachya Hovhannisyan; Pourkazemi Mohammad
Abstract
The biological activity of Persian sturgeon (PS) enfolded crude recombinant growth hormone (rGH) recovered from inclusion bodies (IB) estimated by administrating to fish fingerlings. The psrGH IBs expressed in E. coli were dissolved in cold guanidine HCl solution, immediately diluted ~1000 fold in cold ...
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The biological activity of Persian sturgeon (PS) enfolded crude recombinant growth hormone (rGH) recovered from inclusion bodies (IB) estimated by administrating to fish fingerlings. The psrGH IBs expressed in E. coli were dissolved in cold guanidine HCl solution, immediately diluted ~1000 fold in cold sodium chloride 0.9% solution do not allow the rGH folding and administrated to fishes. It was revealed that intramuscularly injections of rGH at dosage of 0.5 μg/ g once a week for 8 weeks significantly accelerate the growth of fishes. Comparison of the mean growth rate and daily weight and length gain dates of Persian sturgeon fingerlings administrated by pure and crude grade rGH revealed the existence at list 2-5% potentially biologically active psrGH molecules in IBs. This fact enables to avoid the time and cost consuming processes of refolding and purification of rGHs.
Ehsan Nasr; Mohammad Pourkazemi; Hrachia Hovhannisyan
Abstract
Administering growth hormone increases growth rate of cultured fish. The aim of this study was isolation and synthesize of Persian sturgeon GH cDNA. The total RNA was extracted from pituitary gland of Persian sturgeon and cDNA was synthesized. The full-length cDNA sequence of Persian sturgeon contains ...
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Administering growth hormone increases growth rate of cultured fish. The aim of this study was isolation and synthesize of Persian sturgeon GH cDNA. The total RNA was extracted from pituitary gland of Persian sturgeon and cDNA was synthesized. The full-length cDNA sequence of Persian sturgeon contains a 645 nucleotide open reading frame, which encoding a 214 amino acid residues. The position of the signal peptide cleavage site was predicted to be at position 72. After cleaving of a signal peptide of 24 amino acid resi dues, a mature peptide of 190 aa formed. The blast observed that the PS pre GH have highest nucleotide sequence similarity with Acipenseridae family and mammals GH. Secondary structure and tertiary structure of Persian sturgeon growth hormone gene were prediction by online software. The secondary structure of the GH was revealed the predomination of a- helix (> 55%), the domains of high conservation across the vertebrate GH protein. The predicted 3D structure of Persian sturgeon growth hormone attribute to the typical 4-a-helix bundle protein conformation, the characteristic 3-D confirmation of growth hormones.
