Amin Moqadami; Mohammad Khalaj-Kondori
Abstract
Long non-coding RNAs (lncRNAs) have recently emerged as effective regulatory agents in biological processes as well as in the formation of tumors. LncRNAs are important regulators of cell transformation and cancer progression. LncRNA NEAT1 is one of the most important lncRNAs, and its deregulation has ...
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Long non-coding RNAs (lncRNAs) have recently emerged as effective regulatory agents in biological processes as well as in the formation of tumors. LncRNAs are important regulators of cell transformation and cancer progression. LncRNA NEAT1 is one of the most important lncRNAs, and its deregulation has been reported in a variety of human cancers. Ovarian cancer has an inverse relationship with the number of reported pregnancies and deliveries, while it has a direct relationship with infertility. This study aimed to investigate NEAT1 expression in ovarian cancer. A total of 140 tissue samples, including 70 ovarian tumors and 70 marginal samples, were included in the study. Total RNA was extracted using the RNXplus solution. The quality and quantity of the extracted RNAs were determined using gel electrophoresis and a NanoDrop device. The complementary DNA was synthesized by the reverse transcriptase enzyme, and quantitative reverse transcriptase PCR was used to quantify the expression of NEAT1. A comparison between the mean expression of NEAT1 in ovarian tumors and marginal samples showed an increase in NEAT1 expression in tumor tissue that was not statistically significant (P-value = 0.2). ROC curve analysis also showed that NEAT1 expression level might not be an informative biomarker for ovarian cancer.
Seyedeh Nahid Fotuhi; Mohammad Khalaj-Kondori; Hadis Karimi
Abstract
Patients with ovarian cancer are mostly diagnosed at advanced stages which leads to poor prognosis and high mortality rate. Deregulation of lncRNA HOXD-AS1 expression associates with cancer development and metastasis. However, the expression level of this lncRNA in ovarian cancer ...
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Patients with ovarian cancer are mostly diagnosed at advanced stages which leads to poor prognosis and high mortality rate. Deregulation of lncRNA HOXD-AS1 expression associates with cancer development and metastasis. However, the expression level of this lncRNA in ovarian cancer is not determined.50paired ovarian tumors and their adjusted normal tissues were included in the study. Total RNA was extracted by TRIzol® Reagent and reverse-transcribed to cDNA using PrimeScript II cDNA synthesis kit. The expression levels of HOXD-AS1 were quantified by qRT-PCR and compared. The Roc curve analysis was used to evaluate the capacity of HOXD-AS1 as a biomarker for ovarian cancer. We observed that lncRNA HOXD-AS1 was significantly upregulated in ovarian tumors compared to their adjusted normal tissues (p <0.003). Moreover, the ROC curve analysis revealed that the lncRNA HOXD-AS1 expression level could discriminate tumoral and non-tumoral tissues with 85% sensitivity and 88% specificity. The lncRNA HOXD-AS1 expression level might be considered as a potential biomarker for ovarian cancer development.
Nazanin Mardokh Rohani; Mohammad Khalaj-Kondari; Majid Khodayi-Shahrak; Mahnaz Talebi; Mohammad Ali Hoseinpur Feizi
Abstract
Recent genome-wide association studies have introduced several genetic variants which contribute to the late-onset Alzheimer's disease (LOAD). Polymorphisms of CHAT, TOMM40, and SORL1 genes have been reported to be associated with the LOAD phenotype. This study was endeavored to evaluate ...
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Recent genome-wide association studies have introduced several genetic variants which contribute to the late-onset Alzheimer's disease (LOAD). Polymorphisms of CHAT, TOMM40, and SORL1 genes have been reported to be associated with the LOAD phenotype. This study was endeavored to evaluate the association of the CHAT rs3810950, TOMM40 rs1160985 and SORL1 rs11218304 polymorphisms with the LOAD in the Turkish-speaking Azeri population of northwest Iran. In a case-control study, we included 174 cases: 88 cases with LOAD diagnosis and 86 healthy individuals. Peripheral blood samples were collected and the genomic DNA of all participants were extracted. Genotyping was carried out by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. We did not observe any significant association between the CHAT rs3810950 and SORL1 rs11218304 alleles with the LOAD. However, both the TOMM40 rs1160985 minor allele T and TT genotype showed significant negative associations with the LOAD. Hence, the TOMM40 rs1160985 polymorphism could be considered as a protective genetic factor against the LOAD in the Turkish-speaking Azeri population of northwest Iran.
Elham Abedheydari; Mohammad khalaj-kondori; Mohammad-Ali Hosseinpour-Faizi; Morteza Kosari-Nasab
Abstract
Gene delivery might be affected by several tribulations based on carrier/vector applied. Bacteriophages lambda and M13 have different genome conformations; linear double-stranded and circular single-stranded respectively. Therefore, it might be expected that these two common classes of gene delivery ...
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Gene delivery might be affected by several tribulations based on carrier/vector applied. Bacteriophages lambda and M13 have different genome conformations; linear double-stranded and circular single-stranded respectively. Therefore, it might be expected that these two common classes of gene delivery vehicles will have different capacity for gene delivery and expression in eukaryote cells. To address the possible effects of linear double-stranded and circular single-stranded genome conformations of bacteriophages lambda and M13 on the transgene expression, the transfection efficacy of two vectors based on lambda and M13 were compared in AGS cell line. The GFP encoding sequence was inserted into the Lambda ZAP-CMV XR vector which resulted in λ-ZAP-CMV-GFP construct. The construct was then in vitro packaged using Gigapack® III Gold packaging extract and λ-GFP phage particles were obtained. The λ-GFP phage particles were then used for in vivo excisioning which resulted in M13-CMV-Script-GFP construct. 1011 copy of λ-ZAP-CMV-GFP or M13-CMV-Script-GFP constructs were transfected into AGS cells using lipofectamine 2000. Transfection efficiencies were analyzed by FACS. Results showed that linear double-stranded λ-ZAP-CMV-GFP was efficient than single-stranded form of M13-CMV-Script-GFP while its double-stranded form was efficient than the linear double-stranded λ-ZAP-CMV-GFP construct for transgene delivery and expression. Moreover the GFP signals resulted from transfections by single-stranded form of M13-CMV-Script-GFP construct faded more quickly in comparison to others. These findings highlight that genome conformation of gene carriers might be an important factor when seeking for an appropriate gene carrier/vehicle.
