Sepideh sadat Hosseini; Shadi Mehrzad; Halimeh Hassanzadeh; Hamid Reza Bidkhori; Mahdi Mirahmadi; Madjid Momeni-Moghaddam; Fatemeh Sadeghifar; Moein Farshchian
Abstract
Mesenchymal stem/stromal cells (MSCs) as one of the most important types of adult stem cells secrete a variety of immunomodulatory cytokines. However, their immunomodulatory features strongly depend on the molecular cross-talk between cells and the surrounding microenvironment. Hence, ...
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Mesenchymal stem/stromal cells (MSCs) as one of the most important types of adult stem cells secrete a variety of immunomodulatory cytokines. However, their immunomodulatory features strongly depend on the molecular cross-talk between cells and the surrounding microenvironment. Hence, some strategies were proposed to empower their beneficial effects during cell-therapeutic procedures to avoid confusing results. Licensing the cells with chemical compounds could be considered as one of the most applicable methods for induction of anti-inflammatory status in the cells. Human chorionic gonadotropin (hCG) is a pregnancy related hormone which has been shown to be essential for the establishment of a successful pregnancy. HCG supports the implantation of fetus in the maternal endometrium, due to its immunomodulatory effects. Moreover, the regulatory role of hCG has been previously mentioned in case of some autoimmune-based diseases. In the present study, the capacity of this hormone for induction of different immune-encountered genes expression was examined in primary cultures of human adipose tissue derived mesenchymal stem cells (Ad-MSCs). In this regard, Ad-MSCs were exposed to 10 IU of hCG for 72 hours. Molecular studies via quantitative Real-time PCR (qRT-PCR) experiments were performed to detect gene expression modifications based on the application of SYBR Green as the fluorescent dye and in comparison to the RPLP0 as the housekeeping gene. Results confirmed that hCG significantly upregulated TSG-6, TGF-β1, IL-1β and IL-6 expression levels comparing with the control group, while it downregulates COX-2 expression, and had no statistically significant effects on IL-10 andTDO2. In conclusion, priming Ad-MSCs with hCG may enhance the proliferation and immunoregulatory potential of these cells, although it needs further investigations to reveal involved molecular pathways.
Shadi Mehrzad; Sepideh sadat Hosseini; Madjid Momeni-Moghaddam; Moien Farshchian; Halimeh Hassanzadeh; Mahdi Mirahmadi; Fatemeh Sadeghifar; Hamid Reza Bidkhori
Abstract
Oxidative stress occurs as a result of breaking down the balance between oxidants (e.g., reactive oxygen species (ROS)) and antioxidants in cells. Several studies have shown that there is a close relationship between oxidative stress and inflammation at the sites of injury. Mesenchymal ...
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Oxidative stress occurs as a result of breaking down the balance between oxidants (e.g., reactive oxygen species (ROS)) and antioxidants in cells. Several studies have shown that there is a close relationship between oxidative stress and inflammation at the sites of injury. Mesenchymal stem cells (MSCs) are exposed to endogenous and exogenous oxidants generated during their ex vivo expansion or following in vivo transplantation. α-tocopherol (vitamin E) is a fat-soluble compound known for its anti-oxidant and anti-inflammatory properties. In many studies, the immunomodulatory effects of vitamin E have been observed in vivo. This study aimed to determine whether pretreatment of MSCs with antioxidants like vitamin E, will enhance the anti-inflammatory and immunomodulatory properties of these cells. For this purpose, adipose-derived MSCs (ASCs) were treated with vitamin E (600 μM) for 48 h. Quantitative PCR (qPCR) experiments were performed to evaluate the expression of genes related to inflammation (IL-1β, IL-6, IL-17, IL-10) or immunomodulation (TSG-6, COX-2, TDO2, TGF-β1). Results indicated that vitamin E significantly increased the expression of COX-2, TSG-6, and IL-1β genes at the mRNA level compared with the control group, while it significantly decreased IL-6 and TGF-β expressions. No effect was observed for IL-17, IL-10, and TDO2 genes. These results suggest that in vitro preconditioning of ASCs with vitamin E may allow the cells to improve their anti-inflammatory and immunoregulatory capacities. Vitamin E pretreatment could lead to the improvement of their therapeutic abilities in conditions that are influenced by oxidative stress.
Mahdi Mirahmadi; Asieh Heirani-Tabasi; Halimeh Hassanzadeh; Mandana Pishbin; Hamid Reza Bidkhori; Hojjat Naderi-Meshkin
Abstract
Preconditioning (PC), is an approach to improve therapeutic potential of stem cells against ischemic environment. PC has several advantages over other therapeutic techniques as this results in increase of transplanted stem cells recruitment, retention, survival and subsequently the induction of a more ...
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Preconditioning (PC), is an approach to improve therapeutic potential of stem cells against ischemic environment. PC has several advantages over other therapeutic techniques as this results in increase of transplanted stem cells recruitment, retention, survival and subsequently the induction of a more supportive environment within the damaged tissue via secretion of angiogenic factors. Special attention is needed to recognize new materials, compounds, and conditions to assess the feasibility of PC for being applied in clinics to treat the ischemic diseases.
Maryam Moghaddam Matin; Morvarid Saeinasab; Saeideh Nakhaei-Rad; Mahdi Mirahmadi; Nasser Mahdavi Shahri; Mahmoud Mahmoudi; Ahmad Reza Bahrami
Abstract
Abstract
Regeneration is a biological phenomenon, which takes place via two main mechanisms: first,
dedifferentiation of mature cells followed by their differentiation into functional new cells and second,
activation of endogenous somatic stem cells for regeneration of damaged or lost tissues. One ...
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Abstract
Regeneration is a biological phenomenon, which takes place via two main mechanisms: first,
dedifferentiation of mature cells followed by their differentiation into functional new cells and second,
activation of endogenous somatic stem cells for regeneration of damaged or lost tissues. One of the best
examples of healing process in mammals is the regeneration of damaged pinna in rabbits by blastema tissue.
The aim of present study was to investigate culture requirements, proliferative properties and expression of
some stemness factors in cells derived from regenerating blastema tissue obtained from rabbit pinna in vitro.
The regenerating tissues were obtained from male New Zealand white rabbits by double punching of the pinna
and cell culture conditions were set to derive and enrich the self renewing cells for further characterisation. The
cells were subjected to survival and growth examinations in vitro, and expression of several stemness factors
was studied in these cells using reverse transcription polymerase chain reaction (RT-PCR). Results revealed
that the derived cells are rather immortal, as they have been growing for more than 120 passages in culture up
until this report. Furthermore, RT-PCR and flow cytometry analyses showed that these cells express a number
of stemness related genes including Oct4 and Sox2. In conclusion, in this study, stem like cells were derived
from blastema tissue of rabbit ears for the first time, showing great self renewing capacity, which provides a
suitable in vitro model for regeneration studies. Moreover, they could be considered as a good source of stem
like cells for future applications.
