Bahar Shahnavaz; Mohsen Karrabi; Simin Maroof; Mansour Mashreghi
Abstract
The aim of this study was identification and characterization of highly efficient Extracellular Polymeric Substance (EPS) producing bacteria in activated sludge. Among 74 isolated bacteria, 20 EPS-producing bacteria were obtained from wastewater treatment plant of Bojnourd. EPS extraction was performed ...
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The aim of this study was identification and characterization of highly efficient Extracellular Polymeric Substance (EPS) producing bacteria in activated sludge. Among 74 isolated bacteria, 20 EPS-producing bacteria were obtained from wastewater treatment plant of Bojnourd. EPS extraction was performed using absolute ethanol after glucose-enriched culture. Dry weight, concentration, and the ratio of carbohydrate to protein for the EPS of each isolate was then determined. Molecular identification of four bacteria with the highest EPS production was carried out using 16S rRNA gene amplification. The results showed that these bacteria are belonging to the genus Bacillus, Pseudomonas and Klebsiella. EPS productions were studied in different conditions (carbon and nitrogen sources, different levels of glucose and yeast extract and temperature) for the genus Bacillus and Pseudomonas.The production of EPS was observed highest while in the presence of 2.5 to 3% glucose and 0.5% yeast extract at temperature of 30 to 37°C.
Bahar Shahnavaz; Roberto A. Geremia
Abstract
Bacteria play a major role in environmental processes. However, the spatial and seasonal variations and environmental impact factors on different bacterial groups have been poorly studied. In the present study, we compared the spatial and seasonal variations of two bacterial groups (Acidobacteria, Actinobacteria) ...
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Bacteria play a major role in environmental processes. However, the spatial and seasonal variations and environmental impact factors on different bacterial groups have been poorly studied. In the present study, we compared the spatial and seasonal variations of two bacterial groups (Acidobacteria, Actinobacteria) from Early Snow Melt and Late Snow Melt locations in Alpine tundra. The results revealed that pH is the essential factor for structuration of two bacterial groups. The pattern of Acidobacteria is very similar to the overall bacterial communities in our previous study, while both bacterial communities are highly influenced by seasonal variations with an independent pattern.
