Mahnaz Aghdasi; Fariba Fazli; Mohammad Bagher Bagherieh
Abstract
TRR14 is a novel protein important in trehalose (α-D-glucosyl-[1,1]-α-D-glucopyranoside) signaling in Arabidopsis. In this research, we provided evidences to demonstrate that TRR14 plays role in Arabidopsis responses to salt and drought stress.
The transgenic Arabidopsis plants over-expressing TRR14 ...
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TRR14 is a novel protein important in trehalose (α-D-glucosyl-[1,1]-α-D-glucopyranoside) signaling in Arabidopsis. In this research, we provided evidences to demonstrate that TRR14 plays role in Arabidopsis responses to salt and drought stress.
The transgenic Arabidopsis plants over-expressing TRR14 under the control of CaMV 35S promoter were generated. Transformed lines showed higher transcript levels of TRR14 than that of Wild Type (WT) Arabidopsis plants. The RT-PCR results showed that TRR14 transcript level increased markedly by salt and drought stress both in WT and transformed lines. Further experiments indicated that the TRR14 transformed lines have unchanged seed germination, root length and chlorophyll content under stress conditions. In addition activity of oxidative enzymes like peroxidase and catalase were significantly induced in tranformed lines under salt and drought treatments. Thus, the present data indicate that a novel protein, TRR14, is involved in plant salt and drought tolerance.
Eisa B. Kohan; Mohammad B. Bagherieh-Najjar; Mahnaz Aghdasi; Ahmad Reza Bahrami
Abstract
Environmental stresses affect plant growth and cause losses worth hundreds of million dollars of agricultural industry each year. Many genes induced in response to environmental stresses. The DREB1A gene is a stress-inducible transcription factor that its overexpression increased tolerance to environmental ...
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Environmental stresses affect plant growth and cause losses worth hundreds of million dollars of agricultural industry each year. Many genes induced in response to environmental stresses. The DREB1A gene is a stress-inducible transcription factor that its overexpression increased tolerance to environmental stress in transgenic plants. DNA was extracted from Arabidopsis thaliana var. Col.0 plants and DREB1A gene amplified by particular primers. After purification, PCR products were cloned into pGEMT-EASY vector and transferred into E. coli strain DH5α competent cells. Blue/white colonies were analyzed and present of the DREB1A gene revealed by restriction analysis and sequencing test. A 668-bp XbaI/BamHI digested fragment of DREB1A gene from pGEMT::DREB1A construct cloned in pBI121 plasmid. The recombinant plasmids were transferred into Agrobacterium tumefaciens cells strain of LBA4404 and transformed cells were cultured on selective medium that supplied with kanamycin and rifampycin. As a result of gel electrophoresis of Agrobacterium colonies-PCR product, band existing on expected size show that the DREB1A gene was cloned in to pBI121 binary vector. At time, Agrobacterium cells containing of pBI121::DREB1A construct are using for product of environmental stress tolerant plants.
Mahnaz Aghdasi; Henriette Schluepmann; Sjef Smeekens
Abstract
Trehalose is the alpha, alpha-1,1-linked glucose disaccharide. Its metabolism is found in a wide variety of organisms and is seen as evolutionary old. Trehalose metabolites are however present at only very low concentrations and their role in plants are not understood. The physiological effects of 100 ...
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Trehalose is the alpha, alpha-1,1-linked glucose disaccharide. Its metabolism is found in a wide variety of organisms and is seen as evolutionary old. Trehalose metabolites are however present at only very low concentrations and their role in plants are not understood. The physiological effects of 100 mM trehalose on growth and carbon allocation in seedlings are characterized in this paper. Trehalose feeding to Arabidopsis thaliana elicits strong responses. On 100 mM trehalose, seedlings germinate and extend cotyledons but fail to develop primary leaves. The primary roots do not grow beyond 2-3 mm and there is not any starch in root tips. In light, growth arrest on 100 mM trehalose can be rescued by exogenous supply of metabolisable sugar. Trehalose feeding results in anthocyanin accumulation and chlorophyll reduction. Trehalose causes cells of the root extension zone to swell and lysis. Trehalase expression analysis showed, WT seedlings grown on trehalose have 10-fold induced AtTRE1 expression compared to sorbitol treatment.
