Sasan Mohsenzadeh; Maryam Shahrtash; Hasan Mohabatkar
Abstract
In recent years, several plant species have been used as bioindicators, and several tests have been developed
to evaluate the toxicity of the environmental contaminants. In this study, the root length and DNA band pattern
of root tips in maize (Zea mays L.) seedlings were exposed at different concentration ...
Read More
In recent years, several plant species have been used as bioindicators, and several tests have been developed
to evaluate the toxicity of the environmental contaminants. In this study, the root length and DNA band pattern
of root tips in maize (Zea mays L.) seedlings were exposed at different concentration of cadmium pollutant (40
and 80 mg L−1). The results indicated that the root length of maize seedlings reduced with an increasing
cadmium concentration. A random amplification of polymorphic DNA (RAPD) analysis from the extracted
DNA was carried out using twenty three 10-base pair random primers. Eleven primers produced 72 bands
between 221-3044 base pairs in gel electrophoresis. DNA damage became evident as the presence and/or
absence of DNA fragments in the treated samples compared to the control groups. The number of disappearing
bands in profiles increased from 33 at 40 mgL-1 of cadmium concentration to 45 after exposure to 80 mg L−1
cadmium concentration compared to total bands in control. Three new bands appeared in 40 mgL-1 of cadmium
concentration but five in 80 mgL-1 of cadmium concentration. The results showed that RAPD analysis could
be a useful tool for detection of genotoxic effects of cadmium toxicity on plants.
Sasan Mohsenzadeh; Leila Najafi; Zahra Amirghofran; Ahmad Reza Khosravi
Abstract
Abstract
The antineoplastic activities of alcoholic and aqueous extracts of the roots, stems and leaves of Isatis campylocarpa an endemic species of the Brassicaceae family from Iran, investigated on the growth of Jurkat as an acute lymphocytic leukemia cell line, K562 as a chronic myelogenous leukemia ...
Read More
Abstract
The antineoplastic activities of alcoholic and aqueous extracts of the roots, stems and leaves of Isatis campylocarpa an endemic species of the Brassicaceae family from Iran, investigated on the growth of Jurkat as an acute lymphocytic leukemia cell line, K562 as a chronic myelogenous leukemia cell line and Fen as a bladder cancer cell line using colorimetric assay. Results showed that 1 to 200 μg/ml concentrations of all the extracts inhibited the proliferation of the cells and may be it relate to the Indirubin compounds. The maximum effect on the Jurkat cells observed for the aqueous root extract. The effect of the extracts on the Jurkat cells was greater than on the K562 cells, which may be indicate more sensitivity to lymphocytic cells than myeloid ones.
Sasan Mohsenzadeh; Sahar Sadeghi; Hassan Mohabatkar; Ali Niazi
Abstract
Osmotic stress is one of the major factors that significantly reduce yields in dry areas. Plants respond to this
abiotic stress at physiological and molecular levels. Many genes are induced under stress conditions by
transcription factors. Dehydration responsive element binding (DREB) protein is a ...
Read More
Osmotic stress is one of the major factors that significantly reduce yields in dry areas. Plants respond to this
abiotic stress at physiological and molecular levels. Many genes are induced under stress conditions by
transcription factors. Dehydration responsive element binding (DREB) protein is a subfamily of AP2/ERF
transcription factors which control expression of many osmotic stress-inducible genes. In this study, 21 days
old seedlings of Sardari cultivar, dry farming bread wheat transferred into hydroponics culture using Hoagland
solution. Osmotic stress treatments performed with adding 100, 200 and 400 g/l poly-ethylene glycol 6000 to
hydroponics culture to obtain –0.15, –0.49, and –1.76 MPa water potential, respectively. After the seedlings
were withered and colorless, relative water content, dry weight, and photosynthesis measured. In addition, RTPCR,
and cDNA sequencing carried out. Molecular analysis of DREB translated protein sequence performed
by DNAMAN, BLASTN, Pfam and PROSITE software. Results showed that osmotic stress decreased relative
water content, root and shoot dry weight and net photosynthesis rate in comparison to control, significantly (P
< 0.05). Sequence alignment indicated 98% homology with other Triticum aestivum DREB protein mRNA.
There was an AP2 domain in the translated protein with three -sheets and one -helix and contains the Val14
and Glu19 amino acids. An EST Sequence deposited in NCBI GenBank database with the accession number of
ES466900.
