Ferdowsi University of Mashhad
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Production and Purification of Recombinant B Subunit of Vibrio cholerae Toxin in Escherichia coli

Ali Khastar; Majid Jamshidain-Mojaver; Hamidreza Farzin; Masoumeh Jomhori Baloch; Iman Salamatian; Kaveh Akbarzadeh-Sherbaf

Volume 13, Issue 2 , April 2022, , Pages 113-120

https://doi.org/10.22067/jcmr.2022.72096.1018

Abstract
  Cholera toxin B subunit (CTB) is a non-toxic and immunostimulatory component of Vibrio cholera toxin. CTB is one of the most studied protein compounds for adjuvant design. This study aimed to produce and purify recombinant CTB (rCTB) by pET-22a plasmid in Escherichia coli BL21(DE) strain, focusing on ...  Read More