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Younes Aftabi Habib Zarredar Mohammadreza Sheikhi Zahra Khoshkam Abasalt Hosseinzadeh Colagar

Abstract

     Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor and its induction may result in suppressing of cell proliferation in colorectal cancer (CRC). Cucurbitacin D (CucD), E (CucE) and I (CucI) are plant derived metabolites that inhibit cancer cells. This study aimed to evaluate the possible potency of the cucurbitacins for activation of AHR expression in CRC cell lines SW-480 and HT-29. The MTT assay was used to find the LC50 value of the metabolites in the cell lines. Afterward, the cells incubated with the LC50 concentrations and AHR-mRNA expression assessed using RT-PCR. The LC50 values of CucD, CucE, and CucI were 4.5, 6.8, and 3.8 μM in HT-29 cell line and 35, 19, 17.5 μM in SW-480 cells respectively. The SW-480 cells were more resistant against Cucs in comparison with HT-29 cells and all three Cucs cause to more AHR-mRNA expression in HT-29 cells. CucE had the lowest effect on AHR-mRNA expression in the cell lines and CucI was a common metabolite for both HT-29 and SW-480 cells, which showed the lowest LC50 value (the highest toxicity) and the highest effect on AHR-mRNA expression. CucI may have a potential to be used as an activator of AHR expression in CRC studies.

Article Details

Keywords

Aryl hydrocarbon receptor, Colon cancer, Cucurbitacin, HT-29, SW-480

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How to Cite
Younes, A., Habib, Z., Mohammadreza, S., Zahra, K., & Abasalt, H. C. (2019). Induction of AHR Gene Expression in Colorectal Cancer Cell Lines by Cucurbitacin D, E, and I. Journal of Cell and Molecular Research, 10(2), 67-75. https://doi.org/10.22067/jcmr.v10i2.74806
Section
Research Articles