Aspergillus fumigatus is one of the pathogenic filamentous fungi that could cause opportunistic infection, allergy and poisoning. Since, genetic sequencing of some of these fungi has been completed, identifying the protein profile of these fungus cells is necessary. One of these genes is the Annexin family. Annexin C4 is a new member of fungal annexins. This study investigates the effect of ANXC4 mutant gene in proteome profile of Aspergillus Fumigatus. Moreover, in order to enhance the power of protein complex separation, we used an optimized prefractionation method. Using reverse phase-high performance liquid chromatography eight fractions were separated. Then to confirm the protein concentration, each fraction was tested by SDS-PAGE. Protein profile of these fractions was analyzed using 2-DE, and Image Master software. Among the proteins identified statistically, two emerging proteins were observed. The results show that the expression of ANXC4 could affect the expression of some proteins in A.Fumigatus. To accurately identify these proteins, further experiments are needed including Mass spectrometry analysis.
Aspergillus Fumigatus, RP-HPLC, 2-DE, Prefractionation, Proteome profile
Badock, V. Steinhvsen,V.,Bommert,K.,Otto,A. (2001) Prefractionation of protein samples for proteome analysis using reversed-phase high-performance liquid chromatography. Electrophoresis, 22(14), pp. 2856–2864.
Dagenais, T. R. T. and Keller, N. P. (2009) Pathogenesis of Aspergillus fumigatus in invasive aspergillosis. Clinical Microbiology Reviews. American Society for Microbiology (ASM), pp. 447–465.
Debeaupuis, J. P. Sarfati,J.,Chazlet,V.,and paullatge,.J.(1997) Genetic diversity among clinical and environmental isolates of Aspergillus fumigatus. Infection and immunity, 65(8), pp. 3080–5.
Gerke, V. and Moss, S. E. (2002) Annexins: From Structure to Function. Physiological Reviews, 82(2), pp. 331–371.
Govorun, V. M. and Archakov, A. I. (2002) Proteomic technologies in modern biomedical science. Biochemistry (Moscow), pp. 1109–1123.
Karpievitch, Y. V ,.polpitiya,A.D.,Anderson,G.D.,Smith,R.D.,andDabney,A.R. (2010) Liquid chromatography mass spectrometry-based proteomics: Biological and technological aspects. Annals of Applied Statistics, 4(4), pp. 1797–1823.
Khalaj, K., Aminollahi, E., Bordbar, A., and Khalaj, V., Fungal annexins: a mini review (2015)
Khalaj, V. Smith,L.,Brookman,J.,Tuckwell,D. (2004) Identification of a novel class of annexin genes. FEBS Letters, 562(1–3), pp. 79–86.
Khalaj, V. Azarian,B.,Enayati,S.,Vaziri,B. (2011)Annexin C4 in A. Fumigatus: A proteomics approach to understand the function. Journal of Proteomics, 74(10), pp. 1950–1958.
Latgé, J. P. (2001) The pathobiology of Aspergillus fumigatus. Trends in Microbiology, pp. 382–389.
Maryam, M., FU, M, S., Alaiano, A., Camacho, E., Diego, G., Faneuff, E. E. (2019)The enigmatic role of fungal annexins: the case of cryptococcus neoformans
Moss, S. E. and Morgan, R. O. (2004) The annexins. Genome Biology. BioMed Central, p. 219.
Neverova, I. and Van Eyk, J. E. (2005) Role of chromatographic techniques in proteomic analysis. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, pp. 51–63.
Righetti, P. G. Castagna, A.Herbert,B. Reymond,F.Rossier,J.S.(2003) Prefractionation techniques in proteome analysis. in Proteomics, pp. 1397–1407.
Righetti, P. G. Castagna, A.AntonioliL, P.(2005) Prefractionation techniques in proteome analysis: The mining tools of the third millennium. Electrophoresis, pp. 297–319.
Ronning, C. M. Fedorova, N.D., Bowyer, P., Coulson, R., Goldman, G., Kim, H.S., Turner, G., Wortman, J.R., Yu, J., Anderson, M.J., Denning, D.W., Nierman, W.C. (2005)Genomics of Aspergillus fumigatus. Revista Iberoamericana de Micologia, pp. 223–228.
Srinivas, P. R., Verma , M.,Zhao , Y., and Srvastava, S. (2002) Proteomics for cancer biomarker discovery. Clinical Chemistry, pp. 1160–1169.
Van Eyk, J. E. (2001) Proteomics: unraveling the complexity of heart disease and striving to change cardiology. Curr Opin Mol Ther, 3(6), pp. 546–553.
Vaziri, B. ,Rahimpour,M.,Eslami,N.,Fayaz,A.,Rahimian,H. (2006) RP-HPLC prefractionation and its application in expressional proteomics analysis of an in vitro viral infection model. Journal of Separation Science, 29(15), pp. 2284–2291.
This work is licensed under a Creative Commons Attribution 4.0 International License.The AUTHOR hereby authorizes the Journal to use the submitted article for publication, after editorial and reviewing processing. The AUTHOR warrants that the manuscript is the AUTHOR's original work, and authorization of its publication does not violate anyone's rights. The manuscript has not been sent or will not be sent in the future for publication in another Journal. The AUTHOR also warrants that the article contains no libelous or unlawful statements. If the above mentioned work was prepared jointly, the AUTHOR agrees to inform all coauthors of the terms of this agreement and to sign on their behalf.