Mohammad Reza Izadpanah Leila Asadpour


Staphylococcus aureus is a common pathogen potentially able to cause a wide range of infectious diseases in human and animals and coagulase enzyme is one of the important virulence factors of this bacterium. Polymorphism of the coagulase encoding gene (coa) is one of the molecular-based typing methods of S. aureus isolates. In this study, the polymorphism of the coagulase gene among MRSA and MSSA isolates were investigated using PCR-RFLP analysis. To perform coagulase gene typing, the repeated units encoding hypervariable regions of coagulase gene of 30 clinical isolates of S. aureus were amplified by the PCR technique; this was followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP) patterns. In total two amplicons (680 bp and 750 bp) and four distinct RFLP banding patterns (280+400, 340+340, 280+470, and no digested amplicon of 750 bp) were observed. Genotype with PCR-RFLP patterns of 280+400 bp was predominated. The results indicated polymorphism in the investigated regions of coagulase gene. This polymorphism can be used for identification of S. aureus isolates and showing the epidemiological relationship among them.

Article Details

Afrough P., Pourmand M. R., Sarajian A. A. and Saki, M. (2013) Molecular investigation of Staphylococcus aureus, coa and spa genes in Ahvaz hospitals, staff nose compared with patients’ clinical samples. Jundishapur Journal of Microbiology 6(4): 1-7.

Anggraini A. D., Koendhori E. B., Pramono H. and Wahyono D. D. (2017) Polymorphism analysis of the coA (Coagulase) gene in isolates of methicillin-resistant Staphylococcus aureus with AluI restriction sites. Health Science Journal of Indonesia 8(1):1-6.

Babu N. R., Shree G. B., Rekha L. and Karthiga, P. (2014) Molecular Analysis of Coagulase (coa) gene polymorphism in clinical isolates of Staphylococcus aureus by PCR-RFLP. Int J Innov Res Sci Eng Technol 3: 8163-8.

Cheng A. G., McAdow M., Kim H. K., Bae T., Missiakas D. M. and Schneewind O. (2010) Contribution of coagulases towards Staphylococcus aureus disease and protective immunity. PLoS pathogens 6(8): e1001036.

Daum R. S. (2007) Skin and soft-tissue infections caused by methicillin-resistant Staphylococcus aureus. New England Journal of Medicine 357(4): 380-390.

Dinges M. M., Orwin P. M. and Schlievert P. M. (2000) Exotoxins of Staphylococcus aureus. Clinical microbiology reviews 13(1): 16-34.

Foster T. J. (2005) Immune evasion by staphylococci. Nature reviews microbiology 3(12): 948–958.

Himabindu M., Muthamilselvan D. S., Bishi D. K. and Verma, R. S. (2009) Molecular analysis of coagulase gene polymorphism in clinical isolates of methicillin resistant Staphylococcus aureus by restriction fragment length polymorphism based genotyping. Am J Infect Dis 5: 170-176.

Izadpanah M. R. and Asadpour L. (2015) Molecular identification and antibacterial drug resistance pattern of Staphylococcus aureus isolated in Rasht, Iran. Mljgoums 9(3): 40-6.

Ishino K., Tsuchizaki N., Ishikawa J. and Hotta K. (2007) Usefulness of PCR-restriction fragment length polymorphism typing of the coagulase gene to discriminate arbekacin-resistant methicillin-resistant Staphylococcus aureus strains. Journal of clinical microbiology 45(2): 607-609.

Janwithayanuchit I., Ngam-Ululert S., Paungmoung P. and Rangsipanuratn, W. (2006) Epidemiologic study of methicillin-resistant Staphylococcus aureus by coagulase gene polymorphism. Science Asia 32: 127-132.

Mahmoudi H., Arabestani M. R., Mousavi S. F. and Alikhani M. Y. (2017) Molecular analysis of the coagulase gene in clinical and nasal carrier isolates of methicillin-resistant Staphylococcus aureus by restriction fragment length polymorphism. Journal of global antimicrobial resistance 8: 41-45.

McAdow M., Missiakas D. M. and Schneewind O. (2012) Staphylococcus aureus secretes coagulase and von Willebrand factor binding protein to modify the coagulation cascade and establish host infections. Journal of innate immunity 4(2): 141-148.

Muder R. R., Brennen C., Rihs J. D., Wagener M. M., Obman A., Obman A. and Yu V. L. (2006) Isolation of Staphylococcus aureus from the urinary tract: association of isolation with symptomatic urinary tract infection and subsequent staphylococcal bacteremia. Clinical infectious diseases 42(1): 46-50.

Rezaee M. A., Mirkarimi S. F., Hasani A., Sheikhalizadeh V., Soroush M. H. and Abdinia B. (2016) Molecular typing of Staphylococcus aureus isolated from clinical specimens during an eight-year period (2005-2012) in Tabriz, Iran. Archives of Pediatric Infectious Diseases 4(2): e35563.

Saei H. D., Ahmadi M., Mardani K. and Batavani R. A. (2009) Molecular typing of Staphylococcus aureus isolated from bovine mastitis based on polymorphism of the coagulase gene in the north west of Iran. Veterinary microbiology 137(1-2):202-206.

Sajadi S. N., Kaboosi H. and Ghadikolii F. P. (2017) Relationship between antibiotic resistance patterns and coagulase in clinical isolates of Staphylococcus aureus in Nowshahr and Chalous. Zahedan Journal of Research in Medical Sciences 19(11): e55079

Sanjiv K., Kataria A. K., Sharma R. and Singh G. (2008) Epidemiological typing of Staphylococcus aureus by DNA restriction fragment length polymorphism of coa gene. Veterinarski arhiv 78(1): 31-38.

Sarvari J., Bazargani A., Kandekar-Ghahraman M. R., Nazari-Alam A. and Motamedifar M. (2014) Molecular typing of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from Shiraz teaching hospitals by PCR-RFLP of coagulase gene. Iranian journal of microbiology 6(4): 246-252.

Schwarzkopf A. and Karch H. (1994) Genetic variation in Staphylococcus aureus coagulase genes: potential and limits for use as epidemiological marker. Journal of Clinical Microbiology 32(10): 2407-2412.

Shorr A. F., Jackson W. L. and Lipman J. (2006) Making the wrong choice: Consequences for patients with infections due to methicillin-resistant Staphylococcus aureus. Critical care medicine 34(8): 2239-2241.

Talebi-Satlou R., Ahmadi M. and Saei H.D. (2012) Restriction fragment length polymorphism genotyping of Human Staphylococcus aureus isolates from two hospitals in Urmia region of Iran using the coa gene. Jundishapur Journal of Microbiology 5(2):416-20.
How to Cite
Mohammad Reza, I., & Leila, A. (2018). Investigation of coa Gene Polymorphism in Clinical Isolates of Staphylococcus aureus in North of Iran. Journal of Cell and Molecular Research, 10(1), 27-31. https://doi.org/10.22067/jcmr.v10i1.52730
Research Articles