Warning of Reducing Genetic Diversity of Abramis brama (Berg, 1905) in Gilan Coast Using SSR Markers

Document Type : Research Articles

Authors

• Zeinab Hosseinnia
• Ali Shabani
• Hamed Kolangi Miandare

Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

https://doi.org/10.22067/jcmr.v7i2.43997

Abstract

Genetic diversity is one of the three levels of biodiversity. The aim of present study was to compare levels of genetic polymorphism between wild Bream populations using seven microsatellite loci. Genetic diversity was investigated by studying samples collected from two regions, the coast of Chamkhale and Bandaranzali of Gilan province. A total of seven microsatellite loci (MFW7, MFW26, Mcs1EH, Rser10, Bl1-153, Bl2-114 and IC654) were used. The average number of alleles in Chamkhale and Bandaranzali coast were 10 and 10.71 alleles, respectively. The numbers of effective alleles were 7.05 and 7.74 alleles in each population. Allele frequency was declined in wild fish due to inbreeding and genetic drift. The mean of observed heterozygosity values were 0.66 and 0.70 in Chamkhale and Bandaranzali coast, respectively. Approximately, all of loci showed deviation from Hardy-Weinberg equilibrium. The genetic similarity and distance between the two populations were 0.316 and 0.684, respectively. The results of Molecular Variance Analysis revealed that genetic diversity within locations was 97 percent, while among them was 3 percent. The Fst value was 0.024 that indicates the low genetic differentiation between the two locations which could be explained by the low number of alleles in two populations. Furthermore, the Natural Migration (Nm) between two stations was obtained 16.30. According to the analysis, it seems that Abramis brama has not a desirable genetic diversity in the investigated regions.

Keywords

• Bream (Abramis brama orientalis)
• Microsatellite
• Genetic diversity
• Polymorphism

20.1001.1.20089147.2015.7.2.8.1

References

1. Askari Gh. and Shabany A. (2013) Microsatellite loci to determine population structure of Garra rufa (Heckel, 1843) in the Khuzestan Province (Iran). International Journal of Aquatic Biology 1:4: 188-194.

2. Avise J. C. (1994) Molecular Markers, Natural History and Evolution. Chapman and Hall, New York. 551 pp.

3. Bataillon T. M., David J. L. and Schoen D. J. (1996) Neutral genetic markers and conservation: Simulated germplasm collections. Genetics 144: 409-417.

4. Chen L., Li Q. and Yang J. (2008) Microsatellite genetic variation in wild and hatchery populations of the sea cucumber (Apostichopus japonicus Selenka) from northern China. Aquaculture 39: 1541-1549.

5. Coad B. (2014) Fresh water fishes of Iran. Retrieved from http:// www. briancoad.com/ contents.htm.

6. Crooijmans R. P. M. A., Bierbooms V. A.

F., Komen J., Van der poal J. J. and Groenen M. A. M. (1997) Microsatellite markers in common carp (Cyprinus carpio L.). Animal Genetics 28: 129-134.

7. Dewoody J. A. and Avise J. C. (2000) Microsatellite variation in Marin, freshwater and anadromous fishes compare with other animal. Journal of Fish Biology 56 : 61-473.

8. Diz P. A. and Presa P. (2009) The genetic diversity pattern of Mytilus alloprovincialis in Galician Rias (NW Iberian estuaries). Aquaculture 287: 278-285.

9. Ferguson A., Taggart J. B., Prodohl P. A., McMeel O., Thompson C., Stone C., McGinnity P. and Hynes R. A. (1995) The application of molecular markers to the study and conservation of fish populations with special reference to Salmo. Journal of Fish Biology 47: 103-126.

10. Fiumera A. C., Wu L., Parker P. G. and Fuerst P. A. (1999) Effective population size in the captive breeding program of the Lake Victoria cichlid Paralabidochromis chilotes. Zoo Biology 18: 215-222.

11. Frankham R. (2008) Genetic adaptation to captivity in species conservation programs. Molecular Ecology 17: 325-333.

12. Ghasemi A., Keivanshokooh S., Shahriari-Moghadam M., Khara H. and Sourinejad I. (2007) Genetic comparison of Iranian and Azeri populations of the oriental bream Abramis brama orientalis (Berg) using microsatellites. Aquaculture Research 38: 1742-1746.

13. Ghosi Z., Shabani A. and Shaabanpour B. (2011) Genetic diversity of Liza aurata (Risso, 1810) in the coastal regions of Golstan province, using microsatellite marker. Taxonomy and Biosystematics 6:

35-46.

14. Grassi F., Imazio S., Gomarasca S., Citterio S., Aina R., Sgorbati S., Sala F., Patrignani G. and Labra M. (2004) Population structure and genetic variation within Valeriana wallrothii Kreyer in relation to different ecological locations. Plant Science 166: 1437-1441.

15. Hakansson J. and Jensen P. (2005) Behavioural and morphological variation between captive populations of red jungle fowl (Gallus gallus) – possible implications for conservation. Biological Conservation 122: 431-439.

16. Hillis D. M., Mortiz C. and Mable B. K. (1996) Molecular systematic. Sinauer Association Inc. Sunderland, MA. 655 pp.

17. Hosseinnia Z., Shabany A., Kolangi miandare H. (2014) Comparison of genetic variation of wild and farmed Bream (Abramis brama orientalis; berg, 1905) using microsatellite markers. Molecular Biology Research Communications 3: 187-195.

18. Jafari O., Shabany A. and Kolangi Miandare H. (2014) A study of genetic population of Alosa braschnicowi (Borodin, 1904) in Sari and Mahmodabad coasts in the Caspian Sea, using Microsatellite loci. International Journal of Aquatic Biology 2:1, 20-26.

19. Keivanshokouh S. and Ghasemi A. (2009) Genetic diversity of Abramis brama using microsatellite marker. Modern Genetic 5: 47-52.

20. Kiabi B. H., Abdoli A. and Naderi M. (1999) Status of the fish fauna in the south caspian basin of Iran. Zoology in the Middle East 18: 57-65.

21. King T. L., Kalinowski S. T., Schill W. B., Spidle A. P. and Lubinski B. A. (2001) Population structure of Atlantic salmon (Salmo salar L.): a range-wide perspective from microsatellite DNA variation. Molecular Ecology Notes 10: 807-821.

22. Li J., Wang G. and Bai Z. (2009) Genetic variability in four wild and two farmed stocks of the Chinese freshwater pearl mussel (Hyriopsis cumingii) estimated by microsatellite DNA markers. Aquaculture 287: 286-291.

23. Lind C. U., Evans B. S., Knauer J., Taylor J. J. U. and Jerry D. R. (2009) Decreased genetic diversity and a reduced effective population size in cultured silver-lipped pearl oysters (Pinctada maxima). Aquaculture 286: 12-19.

24. Liu Z. J. and Cordes J. F. (2004) DNA marker technologies and their applications in aquaculture genetics. Aquaculture 238: 1–37.

25. Lundrigan T. A., Reist J. D. and Ferguson M. M. (2005) Microsatellite genetic variation within and among Arctic charr (Salvelinus alpinus) from aquaculture and natural populations in North America. Aquaculture 244: 63-75.

26. Neff B. D. and Gross M. R. (2001) Microsatellite evolution in vertebrates: inference from AC dinucleotide repeats. Evolution 55: 1717–1733.

27. Nei M. (1978) Estimation of average heterozygosity and genetic distance from small number of individuals. Genetics 89: 583-590.

28. Nei M., Maruyama T. and Chakraborty R. (1975) The bottleneck effect and genetic variability in populations. Evolution 29: 1-10.

29. O'Connell M. and Wright J. M. (1997) Microsatellite DNA in fishes. Fish Biology 7: 331-363.

30. Oosterhout C. V., Hutchinson W. F., Wills D. P. M. and Shipley P. (2004) MICRO CHECKER: Software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4: 535-538.

31. Park L. K. and Moran P. (1995) Developments in molecular genetic techniques in fisheries. Molecular Genetics in Fisheries. London.

32. Rajora O. P., Rahman M. H., Buchert G. P. and Dancik B. P. (2000) Microsatellite DNA analysis of genetic effects of harvesting in old-growth eastern white pine (Pinus strobus) in Ontario, Canada. Molecular Ecology 9: 339-348.

33. Reed D. H. (2009) when it comes to inbreeding: slower is better. Molecular Ecology 18: 4521-4522.

34. Rezae M., Shabany A., Shaabanpoor B. and Kashiri H. (2010). Study of genetic structure of Rutilus frisii kutum in Golestan province coastal watersusing microsatellite markers. Iranian Scientific Fisheries Journal 19: 151-156.

35. Shaklee J. B., Tamaru C. S. and Waples R. S. (1982) Speciationand evolution of marine fishes studied by electrophoretic analysis of proteins. Pacific Science 36: 141-157.

36. Skalla A., Hbyheim B., Glover K. and Dahle D. (2004) Microsatteletie analysis in domesticated and wild Atlantic salmon. Aquaculture 240: 131-143.

37. Thorpe J. P. (1982) The molecular clock hypothesis: biochemical evolution, genetic differentiation and systematic. Annual Review of Ecology and Systematics 13: 139-168.

38. Weber J. L. and May P. E. (1989) Abundant class of human DNA polymorphisms which can be typed using the polymerase chain-reaction. Am. J. Hum. Genet 44: 388–396.

39. Wright S. (1987) Evolution and the genetics of populations, vol. 4: Variability within and among natural populations. University of Chicago Press, Chicago. 590 pp.

40. Xu Z. h., Primavera J. H., Dela Pena L. D., Pettit P., Belak J., Alcivar-Warren A. (2001) Genetic diversity of wild and cultured Black Tiger Shrimp Penaeus monodon in the Philippines using microsatellites. Aquaculture 199: 13-40.