Neuronal differentiation of mouse amnion membrane derived stem cells in response to neonatal brain conditioned medium.

Document Type : Research Articles

Authors

• ُSheida Shahraki ¹
• Hanieh Jalali ²
• Kazem Parivar ³
• Nasim Hayati Roudbari ³
• Mohammad Nabiuni ⁴
• Zahra Heidari ⁴

¹ Islamic Azad University-Tehran

² Kharazmi University Tehran

³ Islamic Azad University, Tehran

⁴ Kharazmi University, Iran

https://doi.org/10.22067/jcmr.v6i2.37591

Abstract

Background: Amniotic membrane derived stem cells (AMSCs) have considerable advantages to use in regenerative medicine and their anti- inflammatory effects, growth factor secretion and differentiation potential make them suitable candidates for stem cell therapy of nervous system. The developing and neonatal brain contains a spectrum of growth factors to direct development of endogenous and donor cells. Using an in vitro model system, we investigated the plasticity and potential of mouse AMSCs to differentiate into neural cells in response to neonatal mice brain extracted medium.
Methods: Mouse amniotic membrane stem cells were isolated from embryos, cultured in presence of medium derived from neonatal mouse brain medium and immunohistochemistry and flow cytometry analyses were used to explore the neural differentiation of them.
Results: Isolated amnion membrane stem cells showed high rate of viability and proliferation and presented neural characters in the presence of neonatal brain extracted medium such morphological changes and Nestin and Map-2 expression.
Conclusion: In conclusion, results from this study showed that amnion membrane derives stem cells are potent stem cells to respond to environmental signals promoting them to neural fate.

Keywords

• Brain Extract
• Amniotic Membrane Derived Stem Cells
• Neural Differentiation
• Amnion Membrane
• In Vitro Model System

20.1001.1.20089147.2014.6.2.8.4

References

Akle C. A., Adinolfi M., Welsh K. I., Leibowitz S. and McColl I. (1981) Immunogenicity of human amniotic epithelial cells after transplantation into volunteers. Lancet 2:1003-1005.

Banas R. A., Trumpower C., Bentlejewski C., Marshall V., Sing G. and Zeevi A. (2008) Immunogenicity and immunomodulatory effects of amnion-derived multipotent progenitor cells. Human immunology 69:321-328.

Broughton B. R., Lim R., Arumugam T. V., Drummond G. R., Wallace E. M. and Sobey C. G. (2012) Post-stroke inflammation and the potential efficacy of novel stem cell therapies: focus on amnion epithelial cells. Frontiers in cellular neuroscience 6:66.

Campbell K., Olsson M. and Bjorklund A. (1995) Regional incorporation and site-specific differentiation of striatal precursors transplanted to the embryonic forebrain ventricle. Neuron 15:1259-1273.

Dobreva M. P., Pereira P. N., Deprest J. and Zwijsen A. (2010) On the origin of amniotic stem cells: of mice and men. The International journal of developmental biology 54:761-777.

Fishell G. (1995) Striatal precursors adopt cortical identities in response to local cues. Development 121:803-812.

Friedman W. J., Olson L. and Persson H. (1991) Cells that Express Brain-Derived Neurotrophic Factor mRNA in the Developing Postnatal Rat Brain. The European journal of neuroscience 3:688-697.

Ilancheran S., Michalska A., Peh G., Wallace E. M., Pera M. and Manuelpillai U. (2007) Stem cells derived from human fetal membranes display multilineage differentiation potential. Biology of reproduction 77:577-588.

Izumi M., Pazin B. J., Minervini C. F., Gerlach J., Ross M. A., Stolz D. B., Turner M. E., Thompson R. L. and Miki T. (2009) Quantitative comparison of stem cell marker-positive cells in fetal and term human amnion. Journal of reproductive immunology 81:39-43.

Kakishita K., Elwan M. A., Nakao N., Itakura T. and Sakuragawa N. (2000) Human amniotic epithelial cells produce dopamine and survive after implantation into the striatum of a rat model of Parkinson's disease: a potential source of donor for transplantation therapy. Experimental neurology 165:27-34.

Kawasaki H., Mizuseki K., Nishikawa S., Kaneko S., Kuwana Y., Nakanishi S., Nishikawa S. I. and Sasai Y. (2000) Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity. Neuron 28:31-40.

Kim E. Y., Lee K. B. and Kim M. K. (2014) The potential of mesenchymal stem cells derived from amniotic membrane and amniotic fluid for neuronal regenerative therapy. BMB reports 47:135-140.

Kong X. Y., Cai Z., Pan L., Zhang L., Shu J., Dong Y. L., Yang N., Li Q., Huang X. J. and Zuo P. P. (2008) Transplantation of human amniotic cells exerts neuroprotection in MPTP-induced Parkinson disease mice. Brain research 1205:108-115.

Kopen G. C., Prockop D. J. and Phinney D. G. (1999) Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains. Proceedings of the National Academy of Sciences of the United States of America 96:10711-10716.

Maisonpierre P. C., Belluscio L., Friedman B., Alderson R. F., Wiegand S. J., Furth M. E., Lindsay R. M. and Yancopoulos G. D. (1990) NT-3, BDNF, and NGF in the developing rat nervous system: parallel as well as reciprocal patterns of expression. Neuron 5:501-509.

Mao Y. and Lee A. W. (2005) A novel role for Gab2 in bFGF-mediated cell survival during retinoic acid-induced neuronal differentiation. The Journal of cell biology 170:305-316.

Marcus A. J., Coyne T. M., Black I. B. and Woodbury D. (2008a) Fate of amnion-derived stem cells transplanted to the fetal rat brain: migration, survival and differentiation. Journal of cellular and molecular medicine 12:1256-1264.

Marcus A. J., Coyne T. M., Rauch J., Woodbury D. and Black I. B. (2008b) Isolation, characterization, and differentiation of stem cells derived from the rat amniotic membrane. Differentiation; research in biological diversity 76:130-144.

Michalczyk K. and Ziman M. (2005) Nestin structure and predicted function in cellular cytoskeletal organisation. Histology and histopathology 20:665-671.

Miki T., Lehmann T., Cai H., Stolz D. B. and Strom S. C. (2005) Stem cell characteristics of amniotic epithelial cells. Stem Cells 23:1549-1559.

Niknejad H., Peirovi H., Ahmadiani A., Ghanavi J. and Jorjani M. (2010) Differentiation factors that influence neuronal markers expression in vitro from human amniotic epithelial cells. European cells & materials 19:22-29.

Park D., Xiang A. P., Mao F. F., Zhang L., Di C. G., Liu X. M., Shao Y., Ma B. F., Lee J. H., Ha K. S., Walton N. and Lahn B. T. (2010) Nestin is required for the proper self-renewal of neural stem cells. Stem Cells 28:2162-2171.

Park S. B., Seo M. S., Kim H. S. and Kang K. S. (2012) Isolation and characterization of canine amniotic membrane-derived multipotent stem cells. PloS one 7:e44693.

Pratama G., Vaghjiani V., Tee J. Y., Liu Y. H., Chan J., Tan C., Murthi P., Gargett C. and Manuelpillai U. (2011) Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications. PloS one 6:e26136.

Roubelakis M. G., Trohatou O. and Anagnou N. P. (2012) Amniotic fluid and amniotic membrane stem cells: marker discovery. Stem cells international 2012:107836.

Sakuragawa N., Kakinuma K., Kikuchi A., Okano H., Uchida S., Kamo I., Kobayashi M. and Yokoyama Y. (2004) Human amnion mesenchyme cells express phenotypes of neuroglial progenitor cells. Journal of neuroscience research 78:208-214.

Sakuragawa N., Misawa H., Ohsugi K., Kakishita K., Ishii T., Thangavel R., Tohyama J., Elwan M., Yokoyama Y., Okuda O., Arai H., Ogino I. and Sato K. (1997) Evidence for active acetylcholine metabolism in human amniotic epithelial cells: applicable to intracerebral allografting for neurologic disease. Neuroscience letters 232:53-56.

Sanchez C., Diaz-Nido J. and Avila J. (2000) Phosphorylation of microtubule-associated protein 2 (MAP2) and its relevance for the regulation of the neuronal cytoskeleton function. Progress in neurobiology 61:133-168.

Sankar V. and Muthusamy R. (2003) Role of human amniotic epithelial cell transplantation in spinal cord injury repair research. Neuroscience 118:11-17.

Tamagawa T., Ishiwata I., Ishikawa H. and Nakamura Y. (2008) Induced in-vitro differentiation of neural-like cells from human amnion-derived fibroblast-like cells. Human cell 21:38-45.

Toda A., Okabe M., Yoshida T. and Nikaido T. (2007) The potential of amniotic membrane/amnion-derived cells for regeneration of various tissues. Journal of pharmacological sciences 105:215-228.

Zigova T., Song S., Willing A. E., Hudson J. E., Newman M. B., Saporta S., Sanchez-Ramos J. and Sanberg P. R. (2002) Human umbilical cord blood cells express neural antigens after transplantation into the developing rat brain. Cell transplantation 11:265-274.