Analysis of IFN-γ (+874 A/T) and IL-10 (-1082 G/A) genes polymorphisms with risk of schizophrenia.

Document Type : Research Articles

Authors

• Dor Mohammad kordi Tamandani
• Azizoallah Mojahed
• Maryam Najafi

University of Sistan Baluchistan

https://doi.org/10.22067/jcmr.v6i2.34584

Abstract

Schizophrenia is a sophisticated mental disability which has affected nearly1.1% of people all over the world. According to recent researches, the key proteins triggered in the immune system are cytokines which might also be taking part in the pathogenesis of schizophrenia. The aim of this study was to evaluate the relationship between the -1082G/A and +874T/A polymorphisms of IL-10 and IFN-γ genes, respectively, in patients with schizophrenia. Materials and Methods: Total of 94 schizophrenic patients and 97 individuals as control samples were enrolled in this study. All samples were genotyped by amplification mutation refractory system-polymerase chain reaction (ARMS PCR) for candidate SNPs in IFN-γ and IL-10 genes. Results: No significant association was found among various genotypes of IFN-γ and IL-10 in selected SNPs with risk of schizophrenia, As well as there was no significant variation in allelic frequency of IFN-γ and IL-10 genes with the risk of disease. Conclusion: These data suggest that the -1082G/A of IL-10 and +874T/A IFNγ genes are not involved in the development of schizophrenia risk. To validate of this data, suggesting more studies in diverse populations with larger sample size.

Keywords

• Schizophrenia
• IFN-γ
• IL-10
• Gene
• Polymorphism

20.1001.1.20089147.2014.6.2.6.2

References

Almoguera B. R., Lopez-Castroman A. R., Dorado J., Lopez-Rodriguez P., Fernandez-Navarro R., Baca-Garcia P., Fernandez-Piqueras J., Dal-Re R., Abad-Santos F., Llerena A., Ayuso C. (2011) ATA homozigosity in the IL-10 gene promoter is a risk factor for schizophrenia in Spanish females: a case control study. BMC Med Genet 12: 2312-2381.

Blazevski J., Petkovic F., Momcilovic M., Jevtic B., Miljkovic D. and Mostarica Stojkovic M. (2013) High interleukin-10 expression within the central nervous system may be important for initiation of recovery of Dark Agouti rats from experimental autoimmune encephalomyelitis. Immunobiology 218:1192-1199.

Chiang S. S., Schwarz M., Mueller N. (2013) Is T-helper type 2 shift schizophrenia-specific? Primary results from a comparison of related psychiatric disorders and healthy controls. Psychiatry Clin Neurosci 67:228-236.

Ekelund J., Hovatta I., Parker A., Paunio T., Varilo T., Martin R., Suhonen J., Ellonen P., Chan G., Sinsheimer J. S., Sobel E., Juvonen H., Arajarvi R., Partonen T., Suvisaari J., Lonnqvist J., Meyer J. and Peltonen L. (2001) Chromosome 1 loci in Finnish schizophrenia families. Human molecular genetics 10:1611-1617.

Fiorentino D. F., Zlotnik A., Vieira P., Mosmann T. R., Howard M., Moore K. W. and O'Garra A. (1991) IL-10 acts on the antigen-presenting cell to inhibit cytokine production by Th1 cells. Journal of immunology 146:3444-3451.

Freudenreich O. B. M., Henderson D. C., Evins A. E., Fan X.,Walsh J. P., Goff D. C. (2010) Analysis of peripheral immune activation in schizophrenia using quantitative reverse-transcription polymerase chain reaction (RT-PCR). Psychiatry Res 176:(2-3), 99–102.

Frydecka. D., Beszlej. A., Karabon. L., Pawlak-Adamska. E., Tomkiewicz. A., Partyka. A., Misiak. B., Piotrowski. P., Zagdanska. M. and Kiejna A. (2013) IL-2, IL-6, IFN-gamma and TGF-beta genetic polymorphism with respect to susceptibility to schizophrenia. European Psychiatry, 28.

Itoh K. and Hirohata S. (1995) The role of IL-10 in human B cell activation, proliferation, and differentiation. Journal of immunology 154:4341-4350.

Jun T. Y., Pae C. U., Kim K. S., Han H. and Serretti A. (2003) Interleukin-10 gene promoter polymorphism is not associated with schizophrenia in the Korean population. Psychiatry and clinical neurosciences 57:153-159.

Kim H. J., Eom C. Y., Kwon J., Joo J., Lee S., Nah S. S., Kim I. C., Jang I. S., Chung Y. H., Kim S. I., Chung J. H. and Choi J. S. (2012) Roles of interferon-gamma and its target genes in schizophrenia: Proteomics-based reverse genetics from mouse to human. Proteomics 12:1815-1829.

Kordi-Tamandani D. M., Sahranavard R. and Torkamanzehi A. (2012) DNA methylation and expression profiles of the brain-derived neurotrophic factor (BDNF) and dopamine transporter (DAT1) genes in patients with schizophrenia. Molecular biology reports 39:10889-10893.

Ozbey U., Tug E. and Namli M. (2009) Interleukin-10 gene promoter polymorphism in patients with schizophrenia in a region of East Turkey. The world journal of biological psychiatry : the official journal of the World Federation of Societies of Biological Psychiatry 10:461-468.

Paul-Samojedny M., Kowalczyk M., Suchanek R., Owczarek A., Fila-Danilow A., Szczygiel A. and Kowalski J. (2010) Functional polymorphism in the interleukin-6 and interleukin-10 genes in patients with paranoid schizophrenia--a case-control study. Journal of molecular neuroscience : MN 42:112-119.

Paul-Samojedny M., Owczarek A., Suchanek R., Kowalczyk M., Fila-Danilow A., Borkowska P., Kucia K. and Kowalski J. (2011) Association study of interferon gamma (IFN-gamma) +874T/A gene polymorphism in patients with paranoid schizophrenia. Journal of molecular neuroscience : MN 43:309-315.

Peng H. Y., Ku Y. C., Shu B. C. and Lung F. W. (2008) Association between Interleukin-10 Gene Promoter Haplotype and Schizophrenia in a Han-Chinese Study. International journal of biomedical science : IJBS 4:185-191.

Pravica V., Perrey C., Stevens A., Lee J. H. and Hutchinson I. V. (2000) A single nucleotide polymorphism in the first intron of the human IFN-gamma gene: absolute correlation with a polymorphic CA microsatellite marker of high IFN-gamma production. Human immunology 61:863-866.

Rubinov M. and Bullmore E. (2013) Schizophrenia and abnormal brain network hubs. Dialogues in clinical neuroscience 15:339-349.

Smith R. S. and Maes M. (1995) The macrophage-T-lymphocyte theory of schizophrenia: additional evidence. Medical hypotheses 45:135-141.

Yoon HK K. Y. (2012 Feb) The T allele of the interferon-gamma +874A/T polymorphism is associated with bipolar disorder. Nord J Psychiatry. 66:14-18.

Yu L Y. M., Zhao J,Shi YY,Zhao XZ,Yang JD,Liu ZJ,Gu NF,Feng GY,He L. (2004 Nov 1) An association between polymorphisms of the interleukin-10 gene promoter and schizophrenia in the Chinese population. Schizophr Res. 71:179-183.

Genetic diversity and involvement in bread spoilage of Bacillus strains isolated from flour and ropy bread. Letters in applied microbiology 37: 169-173.

Sun F., Wu, D., Qiu, Z., Jin, M. and Li, J. (2010) Development of real-time PCR systems based on SYBR Green for the specific detection and quantification of Klebsiella pneumoniae in infant formula. Food Control 21: 487-491.

Wehrle E., Didier, A., Moravek, M., Dietrich, R. and Märtlbauer, E. (2010) Detection of Bacillus cereus with enteropathogenic potential by multiplex real-time PCR based on SYBR green I. Molecular and cellular probes 24: 124-130.