Genetic analysis of ND4 and ND4L regions of mitochondrial genome in Khorasan native chickens

Document Type : Scientific Reports

Authors

• Morteza Hashemi Attar
• Mohammad Reza Nassiri

Ferdowsi University of Mashhad

https://doi.org/10.22067/jcmr.v6i2.33386

Abstract

Preserving genetic diversity of Iranian native chickens is significant in order to perform breeding programs and improved production. Genetic diversity among close populations can be determined by investigating their phylogenetic relations. Different approaches have been applied to determine the phylogenic relations from which genomic sequencing has been considered the most functional approach. In this project, we aimed to evaluate the phylogeny and genetic nucleotide sequences of ND4, ND4L regions in mitochondrial genome of Khorasan native chickens. Blood samples were collected from randomly 6 Khorasan native chicken populations and DNA were extracted. ND4L, ND4 regions were amplified using specific primers of 913 and 802 bp length, respectively. Sequencing was don according to Sanger method based on automated system. In comparison with other breeds (data extracted from NCBI), phylogeny trees and matrices regarding their genetic diversity with Khorasan native chickens for ND4, ND4L were drawn. Results showed that there is no haplotype difference between the studied samples. Lowest genetic distance was observed between Khorasan native chicken and other Asian chickens i.e. Jiangbian, Lvenwv and Red jungle fowl for the ND4, ND4L genes indicating their close relationship.

Keywords

• khorasan native chicken
• ND4L, ND4
• Mitochondrial DNA
• phylogeny tree

20.1001.1.20089147.2014.6.2.3.9

References

Bruford M. W., Bradley D. G. and Luikart G. (2003) DNA markers re-veal the complexity of livestock domestication. Nature Reviews Genetics 4: 900–10.

Chinnery P. F., Schon E. A. (2003). Mitochondria. J Neurol Neurosurg Psychiatry 74: 1188–1199.

Crawford R. D. (1990) Origin and history of poultry species. In: Poultry Breedingand Genetics. Ed. By RD Crawford. Elsevier, Amsterdam

Hong Y., Arpit M., Gaofeng W., William W., Hauswirth, V. C., Sanford L., Boye J. G. (2013) Next-generation sequencing of mitochondrial targeted AAV transfer of human ND4 in mice. Molecular Vision 2013; 19:1482-1491.

Lolai P. (2001) Study of genetic diversityof Barbuscapito fish in Mazandaran and Gilan Province. M.Sc. Thesis. University of Tarbiat Modares, Tehran, Iran.

Mirhosseini S. Z. (1998) Study genetic diversity of Iranian Silkworm using protein and DNA Markers. Thesis. Ph.D. Tarbiat Modarres University

Mohammadipestebik F., Pirani N., Shoja J., Mohammadhashemi A. (2011) Determination the mtDNA D-loop Sequence in Marandi Native Chicken Population and Its Phylogenic Relationships with Other Breeds.ResearchJournal ofAnimal Sciences 21(2): 1-9.

Pirany N., Mohammadhashemi A., Alijani S., RezazadehGoli R., Ghanbari S. (2011) MolecularAnalysis of Mazandrani native chicken population based on HVR-I region of Mitochondrial DNA. Journal of Agricultural Biotechnology 1: 53-65

Silva P., Guan X., Ho-Shing O., Jones J., Xu J., Hui D., Notter D. and Smith E. (2008). Mitochondrial DNA-based analysis of genetic variation and relatedness among Sri Lankan indigenous chickens and the Ceylon jungle fowl (Gallus lafayetti). International Society for Animal Genetics, Animal Genetics 40: 1–9.

Tavakolian J. (1999) A look at livestock and chicken genetic pool of Iran. Animal Research Institute of Iran.

Zardoya R. and Meyer A., (1996) Phylogenetic Performance of Mitochondrial Protein-Coding Genes in Resolving Relationships among Vertebrates. Mol. Biol. Evol. 13(7): 933-942.

Zhang S., Zhang Y., Zheng X., Chen Y., Deng H., Wang D., Wei Q., Zhang Y., Nie L., Wu Q. (2000) Molecular phylogenetic systematics of twelve species of Aci-penseriformes based on mtDNA ND4L-ND4 gene sequence analysis.SCIENCE IN CHINA: Vol. 43 No. 2:129-139.