Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus

Erami, Elham; Sadeq-zadeh, Fereydoun

doi:10.22067/jcmr.v10i2.73401

Document Type : Research Articles

Authors

Elham Erami ¹
Fereydoun Sadeq-zadeh ²

¹ Pasteur Institute of Iran, Tehran, Iran

² Green Environment and Sustainable Chemistry, Tehran, Iran

https://doi.org/10.22067/jcmr.v10i2.73401

Abstract

Aspergillus fumigatus is one of the pathogenic filamentous fungi that could cause opportunistic infection, allergy and poisoning. Since, genetic sequencing of some of these fungi has been completed, identifying the protein profile of these fungus cells is necessary. One of these genes is the Annexin family. Annexin C4 is a new member of fungal annexins. This study investigates the effect of ANXC4 mutant gene in proteome profile of Aspergillus Fumigatus. Moreover, in order to enhance the power of protein complex separation, we used an optimized prefractionation method. Using reverse phase-high performance liquid chromatography eight fractions were separated. Then to confirm the protein concentration, each fraction was tested by SDS-PAGE. Protein profile of these fractions was analyzed using 2-DE, and Image Master software. Among the proteins identified statistically, two emerging proteins were observed. The results show that the expression of ANXC4 could affect the expression of some proteins in A.Fumigatus. To accurately identify these proteins, further experiments are needed including Mass spectrometry analysis.

Keywords

20.1001.1.20089147.2019.10.2.5.3

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Journal of Cell and Molecular Research

Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus

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Volume 10, Issue 2 - Serial Number 20
March 2019
Pages 90-96

Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus

References

References

Send comment about this article

Volume 10, Issue 2 - Serial Number 20March 2019Pages 90-96

Volume 10, Issue 2 - Serial Number 20
March 2019
Pages 90-96