Ferdowsi University of Mashhad

Document Type : Research Articles

Authors

AJA University of Medical Sciences

Abstract

Osteoarthritis (OA) is the single most prevalent disorder in older adults having a predicted value of 130 million patients in 2050. Several clinical chemotherapeutic approaches are being applied to treat early or late osteoarthritis. It has been recommended that autologous mesenchymal stem cells (MSCs) from OA patients could be the gold standard to treat OA as these cells have high proliferation and chondrocyte lineage differentiation potential. In this work, human MSCs, derived from adipose tissue (Ad-MSCs), loaded on Polyurethane/Hydroxyapatite (PUHA) and Demineralized Bone Matrix (DBM) and their proliferation, differentiation capabilities were determined by MTT assay and Alizarin Red S staining and the expression of mRNA into osteoblast lineage were determined using Real Time PCR . The result showed that MSCs were more viable on PUHA when compared with DBM and the expression of lineage specific markers showed that differentiation potential of PUHA and DBM was not much different. The osteoblast lineage cells were stained positively with Alizarin Red S in completely similar in both groups. Electron microscopy analysis indicated attachment of Ad-MSCs when cultured on the PUHA and DBM. It was concluded that PUHA can be used in clinics as Osteo-inductive scaffold to treat OA easily, however further investigations are required before moving to clinical studies.

Keywords

Baharvand, H., Hashemi, S.M., Ashtiani, S.K., and Farrokhi, A. (2004). Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro. Int J Dev Biol 50: 645-52.
Bahrami, A.R., Ebrahimi, M., Matin, M.M., Neshati, Z., Almohaddesin, M.R., Aghdami, N., and Bidkhori, H.R. (2011). Comparative analysis of chemokine receptor's expression in mesenchymal stem cells derived from human bone marrow and adipose tissue. J Mol Neurosci 44: 178-85.
Brodie, J.C., and Humes, H.D. (2005). Stem cell approaches for the treatment of renal failure. Pharmacol Rev 57: 299-313.
Caplon, A. (2005). Mesenchymal stem cells: Cell based reconstructive therapy in orthopedics. Tissue Eng 11: 1198-211.
Chakkalakal, D.A., Strates, B.S., Garvin, K.L., Novak, J.R., Fritz, E.D., Mollner, T.J., and McGuire, M.H. (2001). Demineralized bone matrix as a biological scaffold for bone repair. Tissue Eng 7: 161-77.
Dexheimer, V., Mueller, S., Braatz, F., and Richter, W. (2011). Reduced reactivation from dormancy but maintained lineage choice of human mesenchymal stem cells with donor age. PloS one 6: e22980.
Friedenstein, A., Chailakhyan, R., and Gerasimov, U. (1987). Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet 20: 263-72.
Garcia-Álvarez, F., Alegre-Aguaron, E., Desportes, P., Royo-Cañas, M., Castiella, T., Larrad, L., and Martinez-Lorenzo, M.J. (2011). Chondrogenic differentiation in femoral bone marrow-derived mesenchymal cells (MSC) from elderly patients suffering osteoarthritis or femoral fracture. Arch Gerontol Geriatr 52: 239-42.
Ghannam, S., Bouffi, C., Djouad, F., Jorgensen, C., and Noël, D. (2010). Immunosuppression by mesenchymal stem cells: mechanisms and clinical applications. Stem Cell Res Ther 1:1.
Hare, J.M., Traverse, J.H., Henry, T.D., Dib, N., Strumpf, R.K., Schulman, S.P., Gerstenblith, G., DeMaria, A.N., Denktas, A.E., and Gammon, R.S. (2009). A randomized, double-blind, placebo-controlled, dose-escalation study of intravenous adult human mesenchymal stem cells (prochymal) after acute myocardial infarction. J Am Coll Cardiol 54: 2277-86.
Hofmann, A., Ritz, U., Verrier, S., Eglin, D., Alini, M., Fuchs, S., Kirkpatrick, C.J., and Rommens, P.M. (2008). The effect of human osteoblasts on proliferation and neo-vessel formation of human umbilical vein endothelial cells in a long-term 3D co-culture on polyurethane scaffolds. Biomaterials 29: 4217-26.
Hosea Blewett, H.J. (2008). Exploring the mechanisms behind S-adenosylmethionine (SAMe) in the treatment of osteoarthritis. Crit Rev Food Sci Nutr 48: 458-63.
Hsieh, W.-C., Liau, J.-J., and Li, Y.-J. (2015). Characterization and cell culture of a grafted chitosan scaffold for tissue engineering. International Journal of Polymer Science 2015.
Jiang, Y., Jahagirdar, B.N., Reinhardt, R.L., Schwartz, R.E., Keene, C.D., Ortiz-Gonzalez, X.R., Reyes, M., Lenvik, T., Lund, T., and Blackstad, M. (2002). Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418: 41-49.
Labusca, L., Zugun-Eloae, F., Shaw, G., Botez, P., Barry, F., and Mashayekhi, K. (2012). Isolation and phenotypic characterisation of stem cells from late stage osteoarthritic mesenchymal tissues. Curr Stem Cell Res Ther7: 319-28.
Lee, J., Cuddihy, M.J., and Kotov, N.A. (2008). Three-dimensional cell culture matrices: state of the art. Tissue Eng Part B Rev14: 61-86.
Levenberg, S., Huang, N.F., Lavik, E., Rogers, A.B., Itskovitz-Eldor, J., and Langer, R. (2003). Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds. PANS 100: 12741-6.
Maetzel, A., Li, L., Pencharz, J., Tomlinson, G., and Bombardier, C. (2004). The economic burden associated with osteoarthritis, rheumatoid arthritis, and hypertension: a comparative study. Ann Rheum Dis 63: 395-401.
Maniatopoulos, C., Sodek, J., and Melcher, A. (1988). Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res 254: 317-30.
Martin, G.J., Jr., Boden, S.D., Titus, L., and Scarborough, N.L. (1999). New formulations of demineralized bone matrix as a more effective graft alternative in experimental posterolateral lumbar spine arthrodesis. Spine 24: 637-45.
Mulliken, J.B., Glowacki, J., Kaban, L.B., Folkman, J., and Murray, J.E. (1981). Use of demineralized allogeneic bone implants for the correction of maxillocraniofacial deformities. Ann Surg 194: 366-72.
Murphy, J.M., Dixon, K., Beck, S., Fabian, D., Feldman, A., and Barry, F. (2002). Reduced chondrogenic and adipogenic activity of mesenchymal stem cells from patients with advanced osteoarthritis. Arthritis Rheum 46: 704-13.
Polo-Corrales, L., Latorre-Esteves, M., and Ramirez-Vick, J.E. (2014). Scaffold design for bone regeneration. J Nanosci Nanotechnol 14: 15-56.
Rosenthal, R.K., Folkman, J., and Glowacki, J. (1999). Demineralized bone implants for nonunion fractures, bone cysts, and fibrous lesions. Clin Orthop Relat Res 364: 61-9.
Supronowicz, P., Gill, E., Trujillo, A., Thula, T., Zhukauskas, R., Perry, R., and Cobb, R.R. (2013). Multipotent adult progenitor cell‐loaded demineralized bone matrix for bone tissue engineering. J Tissue Eng Regen Med 10: 275-83.
Thibault, R.A., Scott Baggett, L., Mikos, A.G., and Kasper, F.K. (2009). Osteogenic differentiation of mesenchymal stem cells on pregenerated extracellular matrix scaffolds in the absence of osteogenic cell culture supplements. Tissue Eng Part A 16: 431-40.
Tran, C., Ouk, S., Clegg, N.J., Chen, Y., Watson, P.A., Arora, V., Wongvipat, J., Smith-Jones, P.M., Yoo, D., and Kwon, A. (2009). Development of a second-generation antiandrogen for treatment of advanced prostate cancer. Science 324: 787-90.
Upton, J., Boyajian, M., Mulliken, J.B., and Glowacki, J. (1984). The use of demineralized xenogeneic bone implants to correct phalangeal defects: a case report. J Hand Surg Am 9: 388-91.
Vilalta, M., Degano, I.R., Bago, J., Gould, D., Santos, M., Garcia-Arranz, M., Ayats, R., Fuster, C., Chernajovsky, Y., and Garcia-Olmo, D. (2008). Biodistribution, long-term survival, and safety of human adipose tissue-derived mesenchymal stem cells transplanted in nude mice by high sensitivity non-invasive bioluminescence imaging. Stem Cells Dev 17: 993-1004.
Wang, L., Li, Y., Zuo, Y., Zhang, L., Zou, Q., Cheng, L., and Jiang, H. (2009). Porous bioactive scaffold of aliphatic polyurethane and hydroxyapatite for tissue regeneration. Biomed Mater 4: 025003.
Yang, W., Both, S.K., Zuo, Y., Birgani, Z.T., Habibovic, P., Li, Y., Jansen, J.A., and Yang, F. (2015). Biological evaluation of porous aliphatic polyurethane/hydroxyapatite composite scaffolds for bone tissue engineering. J Biomed Mater Res A 103: 2251-59.
Yang, Z., Wu, Y., Li, C., Zhang, T., Zou, Y., Hui, J.H., Ge, Z., and Lee, E.H. (2011). Improved mesenchymal stem cells attachment and in vitro cartilage tissue formation on chitosan-modified poly (L-lactide-co-epsilon-caprolactone) scaffold. Tissue Eng Part A 18: 242-51.
Yoon, E., Dhar, S., Chun, D.E., Gharibjanian, N.A., and Evans, G.R. (2007). In vivo osteogenic potential of human adipose-derived stem cells/poly lactide-co-glycolic acid constructs for bone regeneration in a rat critical-sized calvarial defect model. Tissue Eng 13: 619-27.
Zotarelli Filho, I.J., Frascino, L.F., Greco, O.T., de Araújo, J.D., Bilaqui, A., Kassis, E.N., Ardito, R.V., and Bonilla-Rodriguez, G.O. (2013). Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering. journal of Regenerative Medicine and Tissue Engineering 2: 12.
Zuk, P.A., Zhu, M., Ashjian, P., De Ugarte, D.A., Huang, J.I., Mizuno, H., Alfonso, Z.C., Fraser, J.K., Benhaim, P., and Hedrick, M.H. (2002). Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13: 4279-95.
Zuk, P.A., Zhu, M., Mizuno, H., Huang, J., Futrell, J.W., Katz, A.J., Benhaim, P., Lorenz, H.P., and Hedrick, M.H. (2001). Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7: 211-28.
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