Ferdowsi University of Mashhad

Document Type : Research Articles

Authors

1 گروه علوم دامی دانشکده کشاورزی دانشگاه فردوسی

2 دانشگاه شهید باهنر کرمان

Abstract

The purpose of this study was to investigate the polymorphism of K-casein (K-CN), β-Lactoglobulin (B-LG) and leptin (LP) genes in Iranian Holstein cattle by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. DNA was extracted from blood samples of 139 cows using a modified phenol chloroform method. Association between K-CN, B-LG and LP genes’ polymorphism with milk production traits were investigated using mixed procedure of SAS software. The frequencies of AA, AB and BB genotypes for K-CN (0.72, 0.18 and 0.10), B-LG (0.43, 0.28 and 0.29) and LP (0.24, 0.63 and 0.13) were also calculated. Statistical results revealed a significant association between AA and BB genotypes of the K-CN gene with milk production and milk protein percentage, respectively. Also, BB genotype of the B-LG gene and AA genotype of the LP gene showed a significant association with protein percentage and milk production (P

Keywords

1. Bovenhuis H., Van Arendonk J. A. M. and Korver S. (1992) Associations between milk protein polymorphisms and milk production traits. Journal Dairy Science 75: 2549-2559.
2. Buchanan F. C., Fitzsimmons C. J., Vankessel A. G., Thue T. D. and Winkelman-sim D. C. (2002) Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genetic Selection Evolution 34: 105-116.
3. Buchanan F. C., Vankessel A. G., Waldner C., Christensen D. A. and Laarveld B. (2003) An association between a leptin single nucleotide polymorphism and milk and protein yield. Journal Dairy Science 86: 3164-3166.
4. Cardak A. D. (2005) Effects of genetic variants in milk protein on yield and composition of milk from Holstein-Friesian and Simmentaler cows South African Journal of Animal Science 35: 41- 48.
5. Erhardt G. (1996) Detection of a new kappa-casein variant in milk of Pinzgauer cattle. Animal Genetic 27: 105–107.
6. Godovac-zimmermann J., Krause I., Baranyi M., Fischer-frühholz S. and Juszczak J. (1996) Isolation and rapid sequence characterization of two novel bovine B-lactoglobulins. Journal of Protein Chemistry 15: 743-750.
7. Glantz M., Lindmark–Mansson H., Stalhammar H. and Paulsson M. (2011) Effect of polymorphisms in the leptin, leptin receptor, and acyl-coenzyme A: diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on cheese characteristics. Journal Dairy Science 94: 3295- 3304.
8. Guo S. and Thompson E. (1992) Performing the exact test of Hardy-Weinberg proportion for multiple alleles. Biometric 48: 361–372.
9. Grosclaude F., Mahe M. F., Mercier J. C. and Ribadeu- Dumas B. (1972) Characterization des variants genetique des caseines A and B bovine. European Journal of Biology 26: 328- 337.
10. Hamza A. E., Yang Z. P., Wang X. L, Chen R. J., Wu H. T. and Ibrahim A. I. (2011) The Impact of Kappa Casein Gene Polymorphism on Milk Components and Other Productive Performance Traits of Chinese Holstein Cattle. Pakistan Veterinary Journal 31(2): 153-156.
11. Hayes B. J., Bowman P. B., Chamberlain A. C., Verbyla K., and Goddard M. E. (2009) Accuracy of genomic breeding values in multi-breed dairy cattle Populations. Genetics Selection Evolution 24: 41- 51
12. Heidari M., Ahani Azari M., Hasani S., Khanahmadi A. and Zerehdaran S. (2009) Association of genetic variants of β-lactoglobulin gene with milk production in a herd and a superior family of Holstein cattle. Iranian Journal of Biotechnology 7: 1- 4.
13. Hill J. P. (1993) The Relationship Between B-Lactoglobulin Phenotypes and Milk Composition in New Zealand Dairy Cattle. Journal Dairy science 76: 281- 286.
14. Liefers S.C. pas m.f.w., veerkamp r .f., van der Lende t. 2002. Associations between leptin gene polymorphisms and pro-duction, live weight, energy balance, feed intake, and fertili-ty in holstein heifers. Journal of Dairy Science, 85: 227–238.
15. Lunden A., Nilsson M. and Janson L. (1997) Marked effect of B-lactoglobulin polymorphism on the ratio of casein to total protein in milk. Journal Dairy Science 80: 2996–3005.
16. Mitra A., Schlee P., Krause I., Blusch J., Werner T., Balakrishnan C. R. and Pirchner F. 1998 Kappa-casein polymor-phisms in Indian dairy cattle and Buffalo: A new genetic variant in Buffalo. Anim. Biotechnol.9: 81–87.
17. Moravčikova N.,Trakovicka N., Hazuchova E., Bujko J. and Kasarda R. (2012) Associations between polymorphisms in the leptin gene and milk production traits in pinzgau and slovak spotted cattle. The 21st Acta argiculturae Slovenica , Supplement 3: 259 –263.
18. Ng-Kwaihang K. F. (1998) Genetic polymorphism of milk proteins: relationships with production traits, milk composition and technological properties. Canadian Journal of Animal Science 78: 131-147.
19. Prinzenberg E. M., Hiendlede S., Ikonen T. and Erhardt G. (1996) Molecular genetic characterization of new bovine kappa-casein alleles CSN3F and CSN3G and genotyping by PCR-RFLP. Animal Genetic 27: 347-349.
20. Prinzenberg E. M., Jianlin H. and Erhardt G. (2008) Genetic variation in the kappa-casein gene (CSN3) of Chinese yak (Bos grunniens) and phylogenetic analysis of CSN3 sequences in the genus Bos. Journal Dairy Science 91: 198-203.
21. Rachagani S., Gupta I. D., Gupta N. and Gupta S. C. (2006) Genotyping of beta-lactoglobulin gene by PCR-RFLP in Sahiwal and Tharparkar cattle breeds. BMC Genetic 7: 31-34.
22. SAS User’s Guide: Statistics, Version 8.2 Edition (2002). SAS Inst., Inc., Cary, NC.
23. Sharifzadeh A. and Dosti A. (2012) Investigation of leptin gene polymorphism in Iranian native cattle. Bulgarian Journal of Veterinary Medicine 15: 86- 92.
24. Simpson K. J., Bird P., Shaw P. and Nicholas K. (1998) Molecular characterization and hormonedependent expression of the porcine whey acidic protein gene. Journal of Molecular Endocrinlogy 20: 27–35.
25. Soria L. A., Iglesias G. M., Hugurt M. J. and Mirande S. L. (2003) A PCR-RFLP test to detect allelic variants of the bovine kappa-casein gene. Animal Biotecnology 14: 1- 5.
26. Tsiaras A. M., Bargouli G. G., Banos G. and Boscos C. M. (2005) Effect of kappa casein and beta- lactoglobulin loci on milk production traits and reproduction performanced of Holstein cows. Journal Dairy Science 88: 327-334.
27. Vohra V., Bhattacharya T. K., Dayal S., Kumar P. and Sharma A. (2006) Genetic variants of beta-lactoglobulin gene and its association with milk composition traits in riverine buffalo. Journal Dairy Research 73: 499–503.
CAPTCHA Image