Abbas Doosti; Zahra Khoshfetrat
Abstract
Gastric carcinoma (GC) is the third leading cause of malignancy-related deaths worldwide, and Helicobacter pylori is one of the identified causes of gastric cancer. The Cag pathogenicity island (cagA, cagC, virB2) is one of ...
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Gastric carcinoma (GC) is the third leading cause of malignancy-related deaths worldwide, and Helicobacter pylori is one of the identified causes of gastric cancer. The Cag pathogenicity island (cagA, cagC, virB2) is one of the major pathogens of H. pylori, which increased the risk of gastric cancer development. Some studies have shown that H. pylori significantly alters the expression of certain genes in gastric epithelial cells. This study aimed to investigate the expression of the GPR83, CA1, AWP1, and WTAP genes in AGS cells transfected with the recombinant pIRES2-EGFP-cagC vector. The pIRES2-EGFP-cagC and pIRES2-EGFP plasmids (as controls) were transfected into AGS cells by lipofectamine 2000 solution. Then, RNA extraction and cDNA synthesis were performed. The expression of GPR83, CA1, AWP1, and WTAP genes was evaluated using the real-time PCR method. Finally, the expression of each gene was evaluated using SPSS software and t-test independent statistical tests. Our findings indicated that the expression of the GPR83 gene in AGS cells treated with cagC statistically significantly increased compared to control cells (P=0.0327). On the contrary, the WTAP expression was significantly decreased (P=0.0132), whereas the AWP1 and CA1 mRNA expression levels were not statistically significant. This study shows that GPR83 and WTAP genes's expression in the host is significantly altered through cagC gene expression. Hence, it seems that the cagC gene's presence can explain some alterations in the expression of gastric epithelial cell genes and the cause of gastric cancer pathogenesis caused by H. pylori infection.
