Marzieh Taghizadeh; AliAkbar Ehsanpour
Abstract
The effect of CoCl2 as an ethylene synthesis inhibitor on changes of protein pattern was investigated in potato (Solanum tuberosum L.) plants cultivar White Desiree. In vitro grown plants were subjected to MS medium containing 0, 5, 10, 15, 20, 30, 40 and 60 mg/l CoCl2 for 4 weeks. Different concentrations ...
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The effect of CoCl2 as an ethylene synthesis inhibitor on changes of protein pattern was investigated in potato (Solanum tuberosum L.) plants cultivar White Desiree. In vitro grown plants were subjected to MS medium containing 0, 5, 10, 15, 20, 30, 40 and 60 mg/l CoCl2 for 4 weeks. Different concentrations of CoCl2 showed significant effect on the total soluble proteins. Among different concentrations of CoCl2, using 20 mg/l CoCl2 was the best concentration to inhibit ethylene formation and induce potato plant growth. Application of CoCl2 in the culture medium changed the total protein as well as SDS-PAGE and Iso-electric Point Electrophoresis (PI) patterns. Protein pattern in potato tuber did not show any detectable changes.
aliakbar ehsanpour; Behrokh Shojaie; Mohammad Reza Abdi
Abstract
In this study, the effects of gamma rays on changes of proline, sodium and potassium in potato calli
(Solanum tuberosoum L.) cultivar "White Desiree" were investigated. After irradiation of calli by 60 and 80 Gy
of gamma radiation, irradiated and non irradiated calli were transferred to a callus production ...
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In this study, the effects of gamma rays on changes of proline, sodium and potassium in potato calli
(Solanum tuberosoum L.) cultivar "White Desiree" were investigated. After irradiation of calli by 60 and 80 Gy
of gamma radiation, irradiated and non irradiated calli were transferred to a callus production medium
containing 0, 30, 60, 90, 120 and 150 mM NaCl. After 26 days post treatment amounts of proline, Na+ and K+
were measured. The results showed that generally in each of gamma ray treatment, amounts of proline at 120
and 150 mM NaCl were significantly more than 0, 30, 60 and 90 mM salt. A significant difference observed
only at 150 mM NaCl between irradiated and non irradiated calli. This result could be related to the proline
role in osmotic adjustment at high concentration of salt. The amounts of sodium increased by increasing salt
the concentration but the amount of potassium decreased. These results showed that the sodium influx
inhibited the potassium uptake, and may be because of strong inhibitory effect of sodium on the potassium
uptake system or efflux potassium from the cells. The Na+/K+ ratio decreased at 150 mM by 60 and 80 Gy of
gamma radiation.
Fatemeh Rostami; Aliakbar Ehsanpour
Abstract
Potato (Solanum tuberosum L.) auxiliary buds c.v. White Desiree were cultured in MS medium containing 0,
50, 100, 150 and 200 μM concentrations of silver thiosulfate (STS) under in vitro condition. After eight weeks,
the effect of silver ions (Ag+) in the form of silver thiosulfate complex (STS), ...
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Potato (Solanum tuberosum L.) auxiliary buds c.v. White Desiree were cultured in MS medium containing 0,
50, 100, 150 and 200 μM concentrations of silver thiosulfate (STS) under in vitro condition. After eight weeks,
the effect of silver ions (Ag+) in the form of silver thiosulfate complex (STS), as an ethylene action inhibitor,
on chlorophyll contents of leaves, ascorbate peroxidase, guaiacol peroxidase and catalase activities of roots and
leaves were studied. Application of silver (STS) in culture medium increased chlorophyll content comparing to
the control plants significantly. After treatments of potato plants with STS, ascorbate peroxidase and guaiacol
peroxidase activities in roots were higher than shoots while catalase activity was higher in leaves than roots.
However, increasing of STS concentration in the culture medium resulted in higher activities of antioxidant
enzymes with some variations.
