Hamid Reza Shojania; Madjid Momeni Moghaddam; Seyed Ebrahim Hossini
Abstract
Wound healing is a complex biological process in which many molecules, including microRNA molecules, play an essential role in its regulation. It is well-established that reducing miR-155 expression can accelerate wound healing. This study investigated the effect of using nanoparticles loaded with vitamin ...
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Wound healing is a complex biological process in which many molecules, including microRNA molecules, play an essential role in its regulation. It is well-established that reducing miR-155 expression can accelerate wound healing. This study investigated the effect of using nanoparticles loaded with vitamin C on miR-133, collagen I, and III expressions. In this study, first, nanoparticles of albumin protein were produced and then loaded with vitamin C. 3T3 mouse fibroblast cells were affected by these nanoparticles, and cell behavior was investigated to evaluate the toxicity and appropriate doses. In addition, the expression of collagen I and III genes was studied. The results showed that nanoparticles containing vitamin C in 20 µg/ml concentration had a positive effect on collagen I and III expressions compared to the control group. Moreover, we observed a decrease in the expression of miR-133 in comparison to the control group. Therefore, according to the results of this study, it can be argued that nanoparticles containing vitamin C can significantly decrease the expression of the miR-133 gene and lead to collagen I and III gene overexpression in fibroblasts cells, which is directly effective in wound healing.
Tahereh Sanjari; Toktam Hajjar; Madjid Momeni-Moghaddam
Abstract
Mesenchymal stem cells (MSCs) could differentiate into various types of tissues. These cells serve as a backup for the regeneration and repair of tissues or cells after injury. A skin wound is defined as an injury to the skin that needs to be restored. All types of cells in skin especially mesenchymal ...
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Mesenchymal stem cells (MSCs) could differentiate into various types of tissues. These cells serve as a backup for the regeneration and repair of tissues or cells after injury. A skin wound is defined as an injury to the skin that needs to be restored. All types of cells in skin especially mesenchymal stem cells play important role in wound healing process. In particular, paracrine signaling of MSC regulates the cellular responses at the wound site leading to reduction of inflammation, stimulation of angiogenesis and induction of cell migration and proliferation. Because of these abilities, MSCs are one of the most common stem cells for cell therapy in wound healing. This review focuses on the role of MSCs on wound healing process. In addition, major phases of wound repair and challenges of cell therapy are discussed.
Muhammad Irfan-Maqsood
Abstract
Wound care management is a continuous challenging task for researchers and tissue engineers. Skin substitutes (synthetic and natural) have been introduced as emergency replacements/grafts to the damaged skin and a number of problems such as infection, graft rejection, inadequate healing, short shelf ...
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Wound care management is a continuous challenging task for researchers and tissue engineers. Skin substitutes (synthetic and natural) have been introduced as emergency replacements/grafts to the damaged skin and a number of problems such as infection, graft rejection, inadequate healing, short shelf life etc. have reduced their clinical importance as being the ideal skin substitutes. A number of novel ideas have been presented in last decades which have focused on the applications of stem cells as ideal candidate in the development of ideal skin substitutes.
