Marzieh Taghizadeh; Sara Soltanian; Nahid Nasibi
Abstract
The present study was conducted to determine the volatile and non-volatile fractions and the antioxidant and anti-cancer activities of ethanolic extracts of Dracocephalum polychaetum and D. kotschyi. The volatile and non-volatile fractions were investigated by gas chromatography-mass spectrometry (GC-MS) ...
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The present study was conducted to determine the volatile and non-volatile fractions and the antioxidant and anti-cancer activities of ethanolic extracts of Dracocephalum polychaetum and D. kotschyi. The volatile and non-volatile fractions were investigated by gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). The cytotoxicity effect of two ethanol extracts and the major phenolic components has been evaluated on breast and colon cancer cells by the MMT assay. GC-MS of the essential oils identified about 50 compounds, and perillylaldehyde and D-limonene were the main constituents in the essential oils of the two species. Moreover, high-performance liquid chromatography- Diode array detector analysis demonstrated that the ethanolic extract of D. polychaetum and D. kotschyi were the source of phenolic compounds such as rosmarinic acid, protocatechuic acid, naringin, apigenin, syringic acid, epicatechin, chlorogenic acid, thymol, carvacrol, rutin, p-coumaric acid, gallic acid, benzoic acid, cinnamic acid, resorcinol, quercetin, salicylic acid, 4-hydroxybenzoic acid, and ferulic acid. Rosmarinic acid and thymol were the main predominant phenolic constituents in D. kotschyi and D. polychaetum ethanolic extracts. The cytotoxicity effect of D. kotschyi and D. polychaetum ethanol extracts and the major phenolic components including rosmarinic acid, thymol, apigenin, quercetin, and nariginin has been evaluated on breast and colon cancer cells by MMT assay and results indicated IC50 values in the range of 90 to 140 (µg.ml-1) after 48 hours of treatment with ethanol extracts. Among phenolic components, thymol caused the lowest cell viability and Narengin showed the lowest anti-proliferative activity. Both extracts also showed antioxidant activity using DPPH assay. The findings of this research suggest that the Dracocephalum have precious bioactive and natural compounds with significant antioxidant and in vitro anti-cancer activities.
Mohadese Abdoli; Parisa Fathi Rezaei; Kamran Mansouri
Abstract
This study aimed to investigate the cytotoxicity of a probiotic mixture on human breast cancer cell lines. To prepare the mixture, local probiotic bacteria were cultured, and the lyophilized supernatant was applied for downstream experiments. The antioxidant activity, total phenol content (TPC), ...
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This study aimed to investigate the cytotoxicity of a probiotic mixture on human breast cancer cell lines. To prepare the mixture, local probiotic bacteria were cultured, and the lyophilized supernatant was applied for downstream experiments. The antioxidant activity, total phenol content (TPC), and fatty acid composition of the bacterial supernatant (BS) were also measured. The possible cytotoxic/anti-proliferative effect of the probiotic mixture was accessed on both breast cancer cell lines at different concentrations using MTT assay. Furthermore, the apoptosis-inducing effects of the same mixture was studied by DAPI staining. The highest level of antioxidant activity and total phenol content (TPC) were detected for the BS at 3200 µg/ml. According to the GC–MS analysis, linoleic acid (37.40 %) and oleic acid (26.93 %) were identified as the major fatty acids of the BS. The MTT assay showed that the BS has anti-proliferative effects on MDA-MB-231 and MCF-7 cells in a time- and dose-dependent manner (IC50: 3200 μg/ml). The apoptosis-inducing effects of the mixture was confirmed in both cell lines through morphological analyses of the cells’ nucleoli, and the formation of apoptotic bodies. According to these experiments, cytotoxic effects and apoptosis-inducing potential were confirmed for the BS against two human breast cancer cell lines, including MDA-MB-231, and MCF-7. Hence, it could be considered as a suitable anti-cancer agent.
