Mahnaz Kiani; Homa Zarghami; Farshid Memariani; Ali Tehranifar
Abstract
Tissue culture methods provide tools to supplement traditional methods for collection, propagation and
preservation of endangered plant species. In this study, in vitro propagation of Diaphanoptera khorasanica
Rech.f., a rare and threatened plant species with limited distribution range and population ...
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Tissue culture methods provide tools to supplement traditional methods for collection, propagation and
preservation of endangered plant species. In this study, in vitro propagation of Diaphanoptera khorasanica
Rech.f., a rare and threatened plant species with limited distribution range and population was investigated.
This species has a potential as an ornamental plant. Single node explants were provided from both adult and
seedling sources. Several disinfection treatments were tried to permit selection of a suitable method. Different
growth regulators were used for establishment, proliferation and rooting stages. Explants showed the highest
establishment percentage after 5 min treatment with 1% sodium hypochlorite (NaOCl), cultured in MS medium
containing 2.2 μM 6-benzylaminopurine (BAP) and 2.4 μM indole-3-butyric acid (IBA). The highest
proliferation of explants from both adult and seedling source explants was obtained from media supplemented
by BA treatment in contrast to TDZ. No significant differences were found between different concentrations of
BAP and TDZ. Proliferated shoots in TDZ were longer and had more internode length and less vitrification, in
comparison with those in BAP. In vitro rooting of proliferated shoots just induced in liquid half-strength MS
medium and rooting was not observed in solid medium. The shoots that originated from adult plants gave rise
to the highest rooting rate with 4.8μM α-naphthalene acetic acid (NAA) and 2.4 μM, but NAA rooted plantlets
showed higher survival percentage in acclimatization step. This study was aimed towards developing an
efficient protocol for in vitro propagation of D. khorasanica and conservation of this vulnerable species.
Mahnaz Kiani; Homa Zarghami; Ali Tehranifar; Farshid Memariani
Abstract
In vitro methods provide a variety of tools to supplement traditional methods for collection, propagation and
preservation of endangered plant species. In this study, an efficient protocol was developed for in vitro
propagation of Colutea gifana, a rare and endangered plant species with limited reproductive ...
Read More
In vitro methods provide a variety of tools to supplement traditional methods for collection, propagation and
preservation of endangered plant species. In this study, an efficient protocol was developed for in vitro
propagation of Colutea gifana, a rare and endangered plant species with limited reproductive capacity that
grows in a narrow area of Iran. Single node explants were used for a series of experiments to select the
appropriate disinfection method and growth regulators for establishment, proliferation and rooting stages.
Explants showed the highest establishment percent after treatment with 2% Sodium hypochlorite (NaOCl) for
15 min, cultured in MS medium containing 2.2 μM 6-benzylaminopurine (BA) and 1 μM indole-3-butyric acid
(IBA). In proliferation stage, 8.8 μM of BA was more effective cytokinin than Kinetin (Kin) and Thidiazuron
(TDZ) for growth induction of axillary shoots. In vitro rooting of proliferated shoots was induced in halfstrength
MS medium in all concentrations of both tested auxins i.e. IBA and α-naphthalene acetic acid (NAA).
Eighty percent of the plantlets were successfully acclimatized to ex vitro conditions, showed normal
development. These plants were used to replenish declining populations in the collection sites and conserve C.
gifana from extinction.
