Open Journals System
Issue Information: Vol. 8, No. 1,(2016)

Article Title: Analysis of Chalcone Synthase and Chalcone Isomerase Gene Expression in Pigment Production Pathway at Different Flower Colors of Petunia Hybrida


pages: 8-14

DOI: 10.22067/jcmr.v8i1.50406

Abstract
Variegation in flower color is commonly observed in many plant species and also occurs on petunia (Petunia hybrida)
as an ornamental plant. Variegated plants are highly valuable in the floricultural market. To gain a global perspective
on genes differentially expressed in variegated petunia flowers, we investigated the expression of chalcone synthase
(chs) and chalcone isomerase (chi) as two essential genes in biosynthesis pathway of pigment production. Also, we
measured the concentration level of total flavonoids, naringenin chalcone and naringenin to evaluate the probably
relationship between the expression profile of chs and chi genes and the concentration of mentioned pigments. The
results indicated that chalcone synthase and chalcone isomerase expression had different profile in different petal
color of Petunia hybrida. Because red flower color in petunia is related to the synthesis of pelargonidin-based (orange
to red) pigments, our results suggest that the low chalcone synthase and chalcone isomerase expression levels in
white petals reduce dihydrokaempferol formation, thereby inhibiting pelargonidin production. In contrast, the high
expression levels of these genes observed in red petals ensure sufficient anthocyanin yields to make flowers red.

key words:   Real-time PCR; Chs, Chi; Petunia hybrida

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Reception Date: 06/10/2015 , Accept date: 15/07/2016 , Published Date: 01/07/2016

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